trmD:Gene: Difference between revisions
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|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
| Line 21: | Line 23: | ||
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | |{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]] | ||
|} | |} | ||
</noinclude><protect> | </noinclude><protect> | ||
==Nomenclature== | ==Nomenclature== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
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{| id="U46a9e20d4c3a0" class=" tableEdit Gene_nomenclature_table" | |||
|- | |- | ||
!{{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
| | || | ||
''trmD'' | ''trmD'' | ||
|- | |||
!align=left {{table_heading_style}} |Mnemonic | |||
|| | |||
tRNA methyltransferase | |||
|- | |- | ||
!{{table_heading_style}} | | !align=left {{table_heading_style}} |Synonyms | ||
| | || | ||
''ECK2604, b2607, JW2588''<ref name='LIB:Riley_2006'/>, JW2588'' | |||
|- | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15783.U46a9e20d4c3a0&page=15783&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> || | |||
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<!--Nomenclature_notes--></protect> | <!--Nomenclature_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
| Line 53: | Line 61: | ||
<protect> | <protect> | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="S46a9e20dcad31" class=" tableEdit Gene_location_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
|- | |- | ||
| | | | ||
MG1655 | MG1655 | ||
| Line 63: | Line 76: | ||
59.11 minutes, 59.11 minutes | 59.11 minutes, 59.11 minutes | ||
| | | | ||
MG1655: 2743361..2742594<br /> | MG1655: 2743361..2742594<br /><gbrowseImage> | ||
name=NC_000913:2742594..2743361 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11023 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11023 EcoCyc] | ||
{{GBrowseLink|MG1655|NC_000913|2741594|2744361|GBrowse(MG1655)}} | |||
| | | | ||
{{GBrowseFastaLink|MG1655|NC_000913|2742594|2743361|FASTA Dump}} | |||
*[http://biocyc.org/ECOLI/sequence?object=EG11023 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG11023 EcoCyc Sequence Dump] | ||
|- | |- | ||
| | |||
[[REL606]] | |||
| | |||
| | |||
NC_012967: 2666746..2665979<br /><gbrowseImage> | |||
name=NC_012967:2665979..2666746 | |||
source=REL606 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|REL606|NC_012967|2665979|2666746|GBrowse (REL606)}} | |||
| | |||
{{GBrowseFastaLink|REL606|NC_012967|2665979|2666746|FASTA Dump}} | |||
|- | |||
| | |||
[[BW2952]] | |||
| | |||
| | |||
NC_012759: 2628406..2629173<br /><gbrowseImage> | |||
name=NC_012759:2628406..2629173 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|2628406|2629173|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|2628406|2629173|FASTA Dump}} | |||
|- | |||
| | | | ||
W3110 | W3110 | ||
| Line 77: | Line 127: | ||
| | ||
| | | | ||
W3110: 2743995..2743228<br />{{ | W3110: 2743995..2743228<br /><gbrowseImage> | ||
name=NC_007779:2743228..2743995 | |||
source=W3110 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|W3110|NC_007779|2742228|2744995|GBrowse(W3110)}} | |||
| | | | ||
{{GBrowseFastaLink|W3110|NC_007779|2743228|2743995|FASTA Dump}} | |||
|- | |||
| | | | ||
[[DH10B]] | |||
|-class= | | | ||
|[{{SERVER}} | |||
| | |||
DH10B: 2835126..2834359<br /><gbrowseImage> | |||
name=NC_010473:2834359..2835126 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|DH10B|NC_010473|2832825|2836660|GBrowse(DH10B)}} | |||
| | |||
{{GBrowseFastaLink|DH10B|NC_010473|2832825|2836660|FASTA Dump}} | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15783.S46a9e20dcad31&page=15783&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table]</span> || || || || | |||
|} | |} | ||
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<!--Location_notes--></protect> | <!--Location_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="N46a9e20e91358" class=" tableEdit Gene_sequence_features_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|-class= | |- | ||
|[{{SERVER}} | | | ||
Coding Start (SO:0000323) | |||
| | |||
[[MG1655]] | |||
| | |||
2742594 | |||
| | |||
Edman degradation | |||
| | |||
PMID:6337136<br /> | |||
| | |||
|- class="tableEdit_footer" | |||
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|} | |} | ||
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<protect><!--Sequence_notes--></protect> | <protect><!--Sequence_notes--></protect> | ||
====Notes==== | ====Notes==== | ||
| Line 110: | Line 201: | ||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="R46a9e20ed80c6" class=" tableEdit Gene_allele_table dataTable" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
|- | |- | ||
| | |||
trmDD169A | |||
| | |||
| | |||
D169A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDL197A | |||
| | |||
| | |||
L197A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDF171A | |||
| | |||
| | |||
F171A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDP184A | |||
| | |||
| | |||
P184A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDG59A | |||
| | |||
| | |||
G59A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDG91A | |||
| | |||
| | |||
G91A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDR114A | |||
| | |||
| | |||
R114A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDY115A | |||
| | |||
| | |||
Y115A | |||
| | |||
| | |||
Increases Km for S-adenosyl-L- methionine 24-fold | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDG117A | |||
| | |||
| | |||
G117A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDD119A | |||
| | |||
| | |||
D119A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDR121A | |||
| | |||
| | |||
R121A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDL196A | |||
| | |||
| | |||
L196A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDV192A | |||
| | |||
| | |||
V192A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDG141A | |||
| | |||
| | |||
G141A | |||
| | |||
| | |||
Increases Km for S-adenosyl-L- methionine 82-fold | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDR154A | |||
| | |||
| | |||
R154A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDD135A | |||
| | |||
| | |||
D135A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDY136A | |||
| | |||
| | |||
Y136A | |||
| | |||
| | |||
Increases Km for S-adenosyl-L- methionine 68-fold | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDP193A | |||
| | |||
| | |||
P193A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDD128A | |||
| | |||
| | |||
D128A | |||
| | |||
| | |||
Loss of activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDW207A | |||
| | |||
| | |||
W207A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDW207F,H | |||
| | |||
| | |||
W207F,H | |||
| | |||
| | |||
Small decrease in activity | |||
| | |||
| | |||
| | |||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | |||
trmDR208A | |||
| | |||
| | |||
R208A | |||
| | |||
| | |||
Loss of activity; no effect on tRNA binding | |||
| | |||
| | | | ||
| | | | ||
seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | |||
|- | |||
| | | | ||
trmDI204A | |||
| | | | ||
| | |||
I204A | |||
| | | | ||
| | | | ||
Loss of activity; no effect on tRNA binding | |||
| | | | ||
| | | | ||
|-class= | | | ||
|[{{SERVER}} | seeded from [http://www.uniprot.org/uniprot/P0A873 UniProt:P0A873] | ||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15783.R46a9e20ed80c6&page=15783&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || || | |||
|} | |} | ||
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<protect><!--Alleles_notes--></protect> | <protect><!--Alleles_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="V4e04c4e60f840" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.15783.V4e04c4e60f840&page=15783&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
{{BacktoTop}} | {{BacktoTop}} | ||
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{| | ****************************************************************************************** | ||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9e20f923ad" class=" tableEdit Gene_resource_table" | |||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
|- | |- | ||
| | | | ||
JW2588 | JW2588 | ||
| Line 155: | Line 653: | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | | | ||
PMID:16769691 | |||
Status:Clone OK | Status:Clone OK | ||
| Line 162: | Line 660: | ||
Primer 2:CCgGCCATCCCATCATGTTTATG | Primer 2:CCgGCCATCCCATCATGTTTATG | ||
|- | |- | ||
| | |||
[[:Category:Kohara_Phage:E8F2|E8F2]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=438 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | |||
[[:Category:Kohara_Phage:22D7|22D7]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=439 Genobase] | |||
| | |||
PMID:3038334 | |||
|- | |||
| | | | ||
[[pheA:Gene|pheA18::Tn10]] | [[pheA:Gene|pheA18::Tn10]] | ||
| Line 171: | Line 687: | ||
| | | | ||
est. P1 cotransduction: 84% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 84% <ref name='LIB:Nichols_linked_Tn'/> | ||
|- | |- | ||
| | | | ||
[[srlD:Gene|srlD3131::Tn10]] | [[srlD:Gene|srlD3131::Tn10]] | ||
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CAG18642 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40097 CGSC7423]<ref name='LIB:cgsc'/> | CAG18642 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40097 CGSC7423]<ref name='LIB:cgsc'/> | ||
| | | | ||
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br /> | ||
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10 | |||
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===Notes=== | ===Notes=== | ||
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[[EcoCyc]] | |||
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EcoCyc:EG11023 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EcoGene]] | |||
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EcoGene:EG11023 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
|- | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120001012 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947099 GeneID:947099] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB1016 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[ASAP]] | |||
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ASAP:ABE-0008576 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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===Notes=== | ===Notes=== | ||
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<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | |||
[[Category:Gene List:MG1655]] | |||
[[Category:Gene List:W3110]] | |||
[[Category:Genes not in the Keio knockout collection]] | |||
[[Category:Genes in the ASKA clone collection]] | |||
[[Category:trm genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]] | |||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:43, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
trmD |
|---|---|
| Mnemonic |
tRNA methyltransferase |
| Synonyms |
ECK2604, b2607, JW2588[1], JW2588 |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
59.11 minutes, 59.11 minutes |
MG1655: 2743361..2742594 |
||
|
NC_012967: 2666746..2665979 |
||||
|
NC_012759: 2628406..2629173 |
||||
|
W3110 |
|
W3110: 2743995..2743228 |
||
|
DH10B: 2835126..2834359 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2742594 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
trmDD169A |
D169A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDL197A |
L197A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDF171A |
F171A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDP184A |
P184A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDG59A |
G59A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDG91A |
G91A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDR114A |
R114A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDY115A |
Y115A |
Increases Km for S-adenosyl-L- methionine 24-fold |
seeded from UniProt:P0A873 | ||||
|
trmDG117A |
G117A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDD119A |
D119A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDR121A |
R121A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDL196A |
L196A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDV192A |
V192A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDG141A |
G141A |
Increases Km for S-adenosyl-L- methionine 82-fold |
seeded from UniProt:P0A873 | ||||
|
trmDR154A |
R154A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDD135A |
D135A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDY136A |
Y136A |
Increases Km for S-adenosyl-L- methionine 68-fold |
seeded from UniProt:P0A873 | ||||
|
trmDP193A |
P193A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDD128A |
D128A |
Loss of activity |
seeded from UniProt:P0A873 | ||||
|
trmDW207A |
W207A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDW207F,H |
W207F,H |
Small decrease in activity |
seeded from UniProt:P0A873 | ||||
|
trmDR208A |
R208A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
|
trmDI204A |
I204A |
Loss of activity; no effect on tRNA binding |
seeded from UniProt:P0A873 | ||||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2588 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTGGATTGGCATAATTAGCCT Primer 2:CCgGCCATCCCATCATGTTTATG | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 84% [6] | ||
|
Linked marker |
est. P1 cotransduction: % [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Hjalmarsson, KJ et al. (1983) Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli. J. Biol. Chem. 258 1343-51 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).