moaC:Gene: Difference between revisions

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<noinclude>{{TabsTop}}  
<noinclude>{{TabsTop}}  
{{Tab|Quickview|moaC}}{{Tab On|Gene|moaC}}{{Tab|Gene Product(s)|moaC}}{{Tab|Expression|moaC}}{{Tab|Evolution|moaC}}{{Tab|On One Page|moaC}}{{TabsBottom}}
{{Tab|Quickview|moaC}}{{Tab On|Gene|moaC}}{{Tab|Gene Product(s)|moaC}}{{Tab|Expression|moaC}}{{Tab|Evolution|moaC}}{{Tab|On One Page|moaC}}{{TabsBottom}}
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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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==Nomenclature==
==Nomenclature==
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|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
|
||
''moaC''  
''moaC''
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
|
||
 
Molybdenate
|-
|-
!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK0772, b0783, JW0766, chlA3''<ref name='LIB:Riley_2006'/>
''ECK0772, b0783, JW0766, chlA3''<ref name='LIB:Riley_2006'/>
|-
 
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===Notes===
===Notes===
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==Location(s) and DNA Sequence==
==Location(s) and DNA Sequence==
{{BacktoTop}}
<protect>
<protect>
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J46a9b89bd611c"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
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17.63 minutes&nbsp;
17.63 minutes&nbsp;
|
|
MG1655: 817793..818278<br />{{GBrowseFigureByCoord|816793|819278|ecoli|MG1655}}
MG1655: 817793..818278<br /><gbrowseImage>
name=NC_000913:817793..818278
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11666 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11666 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:816793..819278 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|816793|819278|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:817793..818278&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|817793|818278|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11666 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11666 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 811196..811681<br /><gbrowseImage>
name=NC_012967:811196..811681
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|811196|811681|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|811196|811681|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 720761..721246<br /><gbrowseImage>
name=NC_012759:720761..721246
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|720761|721246|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|720761|721246|FASTA Dump}}
|-  
|
|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 818992..819477<br />{{GBrowseFigureByCoord|817992|820477|ecoli_W3110|W3110}}  
W3110: 818992..819477<br /><gbrowseImage>
name=NC_007779:818992..819477
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|817992|820477|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|818992|819477|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
DH10B: 871721..872206<br /><gbrowseImage>
name=NC_010473:871721..872206
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:817992..820477 GBrowse(W3110)]
{{GBrowseLink|DH10B|NC_010473|870751|873176|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:818992..819477&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|870751|873176|FASTA Dump}}
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===Notes===
===Notes===
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==Sequence Features==
==Sequence Features==
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
817796
|
Edman degradation
|
PMID:8361352<br />
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====Notes====
====Notes====
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==Alleles and Phenotypes==
==Alleles and Phenotypes==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="O46a9b89cbebc6"  class=" tableEdit Gene_allele_table dataTable" 
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!CGSC Mutation ID #
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-class='sortbottom'
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&Delta;moaC (Keio:JW0766)
|
deletion
|
deletion
|
 
|
 
|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=107104 CGSC8823]<ref name='LIB:cgsc'/>
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0002673 moaC::Tn5KAN-2] (FB20237)
|
Insertion at nt 387 in Plus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20237]
|
does not contain [[pKD46]]
|-
|
moaCG52A
|
 
|
G52A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCK67A
|
 
|
K67A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCC76A
|
 
|
C76A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCH77A
|
 
|
H77A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCK131A
|
 
|
K131A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCE112A
|
 
|
E112A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCT117P
|
 
|
T117P
|
 
|
Strongly reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCD128A
|
 
|
D128A
|
 
|
Loss of activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCG110A
|
 
|
G110A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
moaCK51A
|
 
|
K51A
|
 
|
Reduced activity
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A738 UniProt:P0A738]
|-
|
''&#916;moaC755::kan''
|
 
|
 
|
 
|
 
|
PMID:16738554
|
[[cgscmutation:101902|CGSC:101902]]
|
 
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Y4e04bf7107710"  class=" tableEdit Genetic_interaction_table dataTable" 
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW0766
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691
 
Status:Clone OK
 
Primer 1:GCCTCGCAACTGACCCATATCAA
 
Primer 2:CCATCATCCGCTTCCACCTTAAA
|-
|
[[:Category:Kohara_Phage:3D4|3D4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=202 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:24F9|24F9]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=203 Genobase]
|
PMID:3038334
|-
|
[[nadA:Gene|nadA57::Tn10]]
|
Linked marker
|
CAG12147 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38017 CGSC7351]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 20% <ref name='LIB:Nichols_linked_Tn'/>
|-
|
zbh-29::Tn10
|
Linked marker
|
CAG18493 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39400 CGSC7353]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 89% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zbi-29::Tn10
 
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===Notes===
===Notes===
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==Accessions in Other Databases==
==Accessions in Other Databases==
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11666 EcoCyc:EG11666] 
EcoCyc:EG11666
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1617 EchoBASE:EB1617] 
EcoGene:EG11666
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b0783 EcoliGenExpDB:b0783] 
RegulonDB:ECK120001609
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C0865 ECOL199310CYC:C0865]
[http://www.ncbi.nlm.nih.gov/gene?term=945397 GeneID:945397]
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Escherichia coli str. K-12 substr. MG1655
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG11666 EcoGene:EG11666]
[[EchoBASE]]
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EchoBASE:EB1617
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://BioCyc.org/ECOO157/new-image?object=MOAC ECOO157CYC:MOAC]
[[ASAP]]
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ASAP:ABE-0002673
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/31225 CGSC:31225] 
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:moa genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:33, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

moaC

Mnemonic

Molybdenate

Synonyms

ECK0772, b0783, JW0766, chlA3[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.63 minutes 

MG1655: 817793..818278
<gbrowseImage> name=NC_000913:817793..818278 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 811196..811681
<gbrowseImage> name=NC_012967:811196..811681 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 720761..721246
<gbrowseImage> name=NC_012759:720761..721246 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 818992..819477
<gbrowseImage> name=NC_007779:818992..819477 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 871721..872206
<gbrowseImage> name=NC_010473:871721..872206 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

817796

Edman degradation

PMID:8361352[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmoaC (Keio:JW0766)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8823[4]

moaC::Tn5KAN-2 (FB20237)

Insertion at nt 387 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20237

does not contain pKD46

moaCG52A

G52A

Reduced activity

seeded from UniProt:P0A738

moaCK67A

K67A

Reduced activity

seeded from UniProt:P0A738

moaCC76A

C76A

Reduced activity

seeded from UniProt:P0A738

moaCH77A

H77A

Reduced activity

seeded from UniProt:P0A738

moaCK131A

K131A

Reduced activity

seeded from UniProt:P0A738

moaCE112A

E112A

Reduced activity

seeded from UniProt:P0A738

moaCT117P

T117P

Strongly reduced activity

seeded from UniProt:P0A738

moaCD128A

D128A

Loss of activity

seeded from UniProt:P0A738

moaCG110A

G110A

Reduced activity

seeded from UniProt:P0A738

moaCK51A

K51A

Reduced activity

seeded from UniProt:P0A738

ΔmoaC755::kan

PMID:16738554[3]

CGSC:101902


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0766

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCGCAACTGACCCATATCAA

Primer 2:CCATCATCCGCTTCCACCTTAAA

3D4

Kohara Phage

Genobase

PMID:3038334[7]

24F9

Kohara Phage

Genobase

PMID:3038334[7]

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 20% [8]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 89% [8]
Synonyms:zbi-29::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11666

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11666

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001609

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945397

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1617

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002673

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Rivers, SL et al. (1993) Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol. Microbiol. 8 1071-81 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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