fklB:Expression: Difference between revisions

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*Medium:  median data from rich and minimal media
*Medium:  median data from rich and minimal media
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):   
*Doubling time (min): Growth Rate(min):   
*other:  
*Growth phase: other:
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emPAI
emPAI
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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PMID:20671182  
PMID:20671182  
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mRNA
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Ecoli K-12
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0.263709677
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Molecules/cell
|
*Medium: 
*Temperature (°C): 
*Doubling time (min): Growth Rate(min):  *
*Growth phase: other:  *
|
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by RNA_Seq
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PMID:20671182
|-
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Protein
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''E. coli'' K-12 [http://ecoliwiki.net/colipedia/index.php/Category:Strain:EMG2 EMG2]
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340
|
molecules/cell
|
*Medium: MOPS glucose minimal
*Temperature (°C): 37
*Doubling time (min):
*Growth phase: Exponential
*OD: OD600 = 0.2 to 0.25 
*other: aerated
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Quantitative protein sequencing
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Spot ID: M138
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PMID: 9298646
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Protein
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''E. coli'' K-12 MG1655
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10247
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molecules/cell/generation
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*Medium: MOPS Complete 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
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Ribosome Profiling
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PMID: 24766808
|-
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Protein
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''E. coli'' K-12 MG1655
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2940
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molecules/cell/generation
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*Medium: MOPS Minimal 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808
|-
|
Protein
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''E. coli'' K-12 MG1655
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5110
|
molecules/cell/generation
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*Medium: MOPS Complete without Methionine 
* Temperature (°C):  37
*Doubling time (min):
* *Growth phase: 
*OD:
*other:
|
Ribosome Profiling
|
|
PMID: 24766808


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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G46ab8bbccd813"  class=" tableEdit Expression_operons_table"   
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!|Transcription unit(s)
!|Transcription unit(s)
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<!--box uid=421aa90e079fa326b6494f812ad13e79.43426.G46ab8bbccd813-->||{{RegulonDBfig|ECK120004360.jpg|FKLB}}
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<protect><gbrowseImage>
<protect><gbrowseImage>
name=NC_000913:4426938..4426978
name=NC_000913:4426938..4426978
source=ecoli
source=MG1655
type=Gene+DNA_+Protein
type=Gene+DNA_+Protein
preset=Nterminus
preset=Nterminus
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!|Type!!Reference!!Notes
!|Type!!Reference!!Notes
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microarray  
microarray
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[http://genexpdb.ou.edu/index.php?query=b4207 GenExpDB:b4207] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://genexpdb.ou.edu/index.php?query=b4207 GenExpDB:b4207] ([[EcoliGenExpDB|EcoliWiki Page]])  
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G7865 EcoCyc:G7865]
EcoCyc:G7865
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EchoBASE]]
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB2396 EchoBASE:EB2396]
EchoBASE:EB2396
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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EcoGene:EG12503
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[http://ecogene.org/geneinfo.php?eg_id=EG12503 EcoGene:EG12503]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[RegulonDB]]
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RegulonDB:ECK120004360
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0013759
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Latest revision as of 19:40, 21 July 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.96E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323[1]

Protein

Ecoli K-12

121.387+/-0.88

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182[2]

mRNA

Ecoli K-12

0.89619+/-0.03804

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182[2]

mRNA

Ecoli K-12

0.263709677

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182[2]

Protein

E. coli K-12 EMG2

340

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: M138

PMID:9298646[3]

Protein

E. coli K-12 MG1655

10247

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[4]

Protein

E. coli K-12 MG1655

2940

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[4]

Protein

E. coli K-12 MG1655

5110

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID:24766808[4]

edit table

Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

fklB

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Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:4426938..4426978 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

[back to top]


Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4207 (EcoliWiki Page)

NCBI GEO profiles for fklB

microarray

GenExpDB:b4207 (EcoliWiki Page)

Summary of data for fklB from multiple microarray studies

edit table


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4426246..4426432) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ08; Well:H9[5]

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<protect>

Notes

Accessions Related to fklB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7865

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2396

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4207

EcoGene

EcoGene:EG12503

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004360

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013759

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed
  2. ↑ 2.0 2.1 2.2 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 4.2 Li, GW et al. (2014) Quantifying absolute protein synthesis rates reveals principles underlying allocation of cellular resources. Cell 157 624-35 PubMed
  5. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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