rlmE:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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*  
*  
****************************************************************************************** -->{{Gene_nomenclature_help}}
****************************************************************************************** -->{{Gene_nomenclature_help}}
{| id="L46a9ef83d8f68"  class=" tableEdit Gene_nomenclature_table"   
{|   id="L46a9ef83d8f68"  class=" tableEdit Gene_nomenclature_table"   


|-
|-
!align=left |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''rlmE''  
''rlmE''
|-
|-
!align=left |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
rRNA large-subunit methylation
|-
|-
!align=left |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK3168, b3179, JW3146, ftsJ, mrsF'', rrmJ<ref name='LIB:Riley_2006'/>
''ECK3168, b3179, JW3146, ftsJ, mrsF'', rrmJ<ref name='LIB:Riley_2006'/>, mrsF, rrmJ''


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*  
*  
****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|   id="B46a9ef84653b0"  class=" tableEdit Gene_location_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46a9ef84653b0"  class=" tableEdit Gene_location_table"   
|-
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-  
|-  
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71.67 minutes&nbsp;
71.67 minutes&nbsp;
|
|
MG1655: 3325686..3325057<br />{{GBrowseFigureByCoord|3325057|3325686|ecoli|MG1655}}
MG1655: 3325686..3325057<br /><gbrowseImage>
name=NC_000913:3325057..3325686
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11507 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG11507 EcoCyc]
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{{GBrowseFastaLink|ecoli|NC_000913|3325057|3325686|FASTA Dump}}
{{GBrowseFastaLink|ecoli|NC_000913|3325057|3325686|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG11507 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG11507 EcoCyc Sequence Dump]  
|-
|
[[BW2952]]
|
|
NC_012759: 3212205..3212834<br /><gbrowseImage>
name=NC_012759:3212205..3212834
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|012759|3212205|NC_3212834|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|012759|3212205|NC_3212834|FASTA Dump}}
|-  
|-  
|
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&nbsp;
&nbsp;
|
|
W3110: 3327519..3326890<br />{{GBrowseFigureByCoord|3326890|3327519|ecoli_W3110|W3110}}
W3110: 3327519..3326890<br /><gbrowseImage>
name=NC_007779:3326890..3327519
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|3325890|3328519|GBrowse(W3110)}}  
{{GBrowseLink|ecoli_W3110|NC_007779|3325890|3328519|GBrowse(W3110)}}  
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|3326890|3327519|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|NC_007779|3326890|3327519|FASTA Dump}}  
|-  
|-  
|
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|
|
DH10B: 3423431..3422802<br />{{GBrowseFigureByCoord|3422802|3423431|ecoli_DH10B|DH10B}}
DH10B: 3423431..3422802<br /><gbrowseImage>
name=NC_010473:3422802..3423431
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseLink|ecoli_DH10B|NC_010473|3421544|3424689|GBrowse(DH10B)}}
{{GBrowseLink|ecoli_DH10B|NC_010473|3421544|3424689|GBrowse(DH10B)}}
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****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|  id="B46a9ef8524068"  class=" tableEdit Gene_sequence_features_table"   
{|  id="B46a9ef8524068"  class=" tableEdit Gene_sequence_features_table"   
|-
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16911.B46a9ef8524068&page=16911&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table]</span> || || || || ||
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|-  
|-  
|
|
''&#916;rrmJ786::kan''
rlmED83A
|
 
|
D83A
|
 
|
5-fold decrease in activity. Increase in Km for 50S subunit and for S- adenosyl-L-methionine. Loss of S- adenosyl-L-methionine binding
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmES197A
|
 
|
S197A
|
 
|
No change in activity. Increase in Km for 50S subunit
|
|


|
|


|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmEE199A
|
|


|
E199A
|
|


|
|
PMID:16738554
16-fold decrease in activity. No change in S-adenosyl-L-methionine binding
|
 
|
|
[[cgscmutation:102804|CGSC:102804]]
 
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-  
|-  
|
|
Line 199: Line 266:
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]  
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]  
|-
|
rlmEY22A
|
|
Y22A
|
|
No change in activity
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmER32A
|
|
R32A
|
|
4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-34
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmER34A
|
|
R34A
|
|
4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-32
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmEK38A
|
|
K38A
|
|
Loss of activity. Almost no change in S-adenosyl-L-methionine binding
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmED124A
|
|
D124A
|
|
Loss of activity. Loss of S- adenosyl-L-methionine binding
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmED136N
|
|
D136N
|
|
2-fold decrease in activity
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmEK164A
|
|
K164A
|
|
Loss of activity. No change in S- adenosyl-L-methionine binding
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmEK189A
|
|
K189A
|
|
9-fold decrease in activity
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmER194A
|
|
R194A
|
|
No change in activity. Increase in Km for 50S subunit
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
rlmEY201A
|
|
Y201A
|
|
5-fold decrease in activity. Almost no change in S-adenosyl-L-methionine binding
|
|
|
seeded from [http://www.uniprot.org/uniprot/P0C0R7 UniProt:P0C0R7]
|-
|
''&#916;rrmJ786::kan''
|
|
|
|
|
PMID:16738554
|
[[cgscmutation:102804|CGSC:102804]]
|
|-
|
rlmE
|
deletion
|
|
Sensitivity to
|
increases sensitivity to bicyclomycin
|
PMID:21357484
|
|
fig 2


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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.16911.Y4e04c296313ed-->
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
*
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Y4e04c296313ed"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.16911.Y4e04c296313ed&page=16911&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
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<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.16911.Y4e04c296313ed--></protect>
<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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****************************************************************************************** -->{{Gene_resources_help}}
****************************************************************************************** -->{{Gene_resources_help}}
{|  id="J46a9ef862af36"  class=" tableEdit Gene_resource_table"   
{|  id="J46a9ef862af36"  class=" tableEdit Gene_resource_table"   
|-
|- {{table_heading_style}}
!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
|-  
|-  
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Primer 2:CCGGGTTTACGCCCGGTCGCTAC  
Primer 2:CCGGGTTTACGCCCGGTCGCTAC  
|-
|
[[:Category:Kohara_Phage:18H7|18H7]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=520 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:14F11|14F11]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=521 Genobase]
|
PMID:3038334
|-  
|-  
|
|
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*  
*  
****************************************************************************************** -->{{Gene_accessions_help}}
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{|   id="D46a9ef86c5ff0"  class=" tableEdit Gene_accessions_table"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="D46a9ef86c5ff0"  class=" tableEdit Gene_accessions_table"   
|-
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11507 EcoCyc:EG11507]
[http://chase.ou.edu/macro/searchlink.php?bnum=b3179 EcoliGenExpDB:b3179]
|
|
   
   
|-  
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])  
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1470 EchoBASE:EB1470]
[http://BioCyc.org/ECOL199310/new-image?object=C3936 ECOL199310CYC:C3936]
|
|
   
   
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b3179 EcoliGenExpDB:b3179]
[http://BioCyc.org/ECOO157/new-image?object=FTSJ ECOO157CYC:FTSJ]
|
|
   
   
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])  
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C3936 ECOL199310CYC:C3936]
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/33511 CGSC:33511]
|
|
   
   
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG11507 EcoGene:EG11507]
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11507 ECOCYC:EG11507]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=FTSJ ECOO157CYC:FTSJ]
[http://www.ncbi.nlm.nih.gov/gene?term=947689 GeneID:947689]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/33511 CGSC:33511]
RegulonDB:
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0010447&formSubmitReturn=1 ASAP:ABE-0010447]
|


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!|Name!!URL!!Comments
!|Name!!URL!!Comments


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[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:rrm genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:rrm genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:rlm genes]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:46, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rlmE

Mnemonic

rRNA large-subunit methylation

Synonyms

ECK3168, b3179, JW3146, ftsJ, mrsF, rrmJ[1], mrsF, rrmJ

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Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

71.67 minutes 

MG1655: 3325686..3325057
<gbrowseImage> name=NC_000913:3325057..3325686 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3212205..3212834
<gbrowseImage> name=NC_012759:3212205..3212834 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3327519..3326890
<gbrowseImage> name=NC_007779:3326890..3327519 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3423431..3422802
<gbrowseImage> name=NC_010473:3422802..3423431 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrrmJ (Keio:JW3146)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10390[3]

rlmED83A

D83A

5-fold decrease in activity. Increase in Km for 50S subunit and for S- adenosyl-L-methionine. Loss of S- adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

rlmES197A

S197A

No change in activity. Increase in Km for 50S subunit

seeded from UniProt:P0C0R7

rlmEE199A

E199A

16-fold decrease in activity. No change in S-adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

rlmED20A

D20A

2-fold decrease in activity. Increase in Km for 50S subunit

seeded from UniProt:P0C0R7

rlmEY22A

Y22A

No change in activity

seeded from UniProt:P0C0R7

rlmER32A

R32A

4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-34

seeded from UniProt:P0C0R7

rlmER34A

R34A

4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-32

seeded from UniProt:P0C0R7

rlmEK38A

K38A

Loss of activity. Almost no change in S-adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

rlmED124A

D124A

Loss of activity. Loss of S- adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

rlmED136N

D136N

2-fold decrease in activity

seeded from UniProt:P0C0R7

rlmEK164A

K164A

Loss of activity. No change in S- adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

rlmEK189A

K189A

9-fold decrease in activity

seeded from UniProt:P0C0R7

rlmER194A

R194A

No change in activity. Increase in Km for 50S subunit

seeded from UniProt:P0C0R7

rlmEY201A

Y201A

5-fold decrease in activity. Almost no change in S-adenosyl-L-methionine binding

seeded from UniProt:P0C0R7

ΔrrmJ786::kan

PMID:16738554[2]

CGSC:102804

rlmE

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484[4]

fig 2

edit table


Notes

Genetic Interactions

[back to top]



Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

[back to top]


See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3146

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCACAGGTAAGAAGCGTTCTGC

Primer 2:CCGGGTTTACGCCCGGTCGCTAC

18H7

Kohara Phage

Genobase

PMID:3038334[6]

14F11

Kohara Phage

Genobase

PMID:3038334[6]

aer-3075::Tn10

Linked marker

CAG12152 = CGSC7438[3]

est. P1 cotransduction: % [7]
Synonyms:zgh-3075::Tn10, zgj-3075::Tn10

sfsB203::Tn10

Linked marker

CAG12072 = CGSC7440[3]

est. P1 cotransduction: 81% [7]
Synonyms:zgj-203::Tn10, zha-203::Tn10

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Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3179

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3936

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:FTSJ

CGSC (EcoliWiki Page)

CGSC:33511

EcoCyc (EcoliWiki Page)

ECOCYC:EG11507

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947689

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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