rlmE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
rlmE |
|---|---|
| Mnemonic |
rRNA large-subunit methylation |
| Synonyms |
ECK3168, b3179, JW3146, ftsJ, mrsF, rrmJ[1], mrsF, rrmJ |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
71.67 minutes |
MG1655: 3325686..3325057 |
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NC_012759: 3212205..3212834 |
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W3110 |
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W3110: 3327519..3326890 |
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DH10B: 3423431..3422802 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔrrmJ (Keio:JW3146) |
deletion |
deletion |
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|
rlmED83A |
D83A |
5-fold decrease in activity. Increase in Km for 50S subunit and for S- adenosyl-L-methionine. Loss of S- adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
rlmES197A |
S197A |
No change in activity. Increase in Km for 50S subunit |
seeded from UniProt:P0C0R7 | ||||
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rlmEE199A |
E199A |
16-fold decrease in activity. No change in S-adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
rlmED20A |
D20A |
2-fold decrease in activity. Increase in Km for 50S subunit |
seeded from UniProt:P0C0R7 | ||||
|
rlmEY22A |
Y22A |
No change in activity |
seeded from UniProt:P0C0R7 | ||||
|
rlmER32A |
R32A |
4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-34 |
seeded from UniProt:P0C0R7 | ||||
|
rlmER34A |
R34A |
4-fold decrease in activity and increase in Km for 50S subunit; when associated with A-32 |
seeded from UniProt:P0C0R7 | ||||
|
rlmEK38A |
K38A |
Loss of activity. Almost no change in S-adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
rlmED124A |
D124A |
Loss of activity. Loss of S- adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
rlmED136N |
D136N |
2-fold decrease in activity |
seeded from UniProt:P0C0R7 | ||||
|
rlmEK164A |
K164A |
Loss of activity. No change in S- adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
rlmEK189A |
K189A |
9-fold decrease in activity |
seeded from UniProt:P0C0R7 | ||||
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rlmER194A |
R194A |
No change in activity. Increase in Km for 50S subunit |
seeded from UniProt:P0C0R7 | ||||
|
rlmEY201A |
Y201A |
5-fold decrease in activity. Almost no change in S-adenosyl-L-methionine binding |
seeded from UniProt:P0C0R7 | ||||
|
ΔrrmJ786::kan |
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|
rlmE |
deletion |
Sensitivity to |
increases sensitivity to bicyclomycin |
fig 2 | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3146 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACAGGTAAGAAGCGTTCTGC Primer 2:CCGGGTTTACGCCCGGTCGCTAC | |
|
Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: % [7] | ||
|
Linked marker |
est. P1 cotransduction: 81% [7] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Tran, L et al. (2011) Single-gene deletion mutants of Escherichia coli with altered sensitivity to bicyclomycin, an inhibitor of transcription termination factor Rho. J. Bacteriol. 193 2229-35 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
