galE:Gene Product(s): Difference between revisions
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|{{Sub}}[[#Localization |Localization]] | |{{Sub}}[[#Localization |Localization]] | ||
|{{Sub}}[[#Sequence|Sequence]] | |{{Sub}}[[#Sequence|Sequence]] | ||
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]] | |||
|{{Sub}}[[#Structure|Structure]] | |{{Sub}}[[#Structure|Structure]] | ||
|{{Sub}}[[#Gene Product Resources|Resources]] | |{{Sub}}[[#Gene Product Resources|Resources]] | ||
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!{{table heading style}} |Standard name | !align=left {{table heading style}} |Standard name | ||
| | || | ||
GalE | GalE | ||
|- | |- | ||
!{{table heading style}} |Synonyms | !align=left {{table heading style}} |Synonyms | ||
| | || | ||
UDP-galactose-4-epimerase | ''UDP-galactose-4-epimerase<ref name='LIB:Riley_2006'/>, B0759<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalD<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK0748, galD, JW0742, b0759'' | ||
|- | |- | ||
!{{table heading style}} |Product description | !align=left {{table heading style}} |Product description | ||
| | || | ||
UDP-glucose 4-epimerase | UDP-glucose 4-epimerase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>; | ||
Component of | Component of [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/> | ||
UDP-galactose 4-epimerase; hexose-1-phosphate uridylyltransferase<ref name='LIB:EcoGene'/> | |||
|- | |- | ||
!{{table heading style}} |EC number (for enzymes) | !align=left {{table heading style}} |EC number (for enzymes) | ||
| | || | ||
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.2 5.1.3.2]<ref name='LIB:Riley_2006'/> | |||
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<protect><!--Nomenclature_notes--></protect> | <protect><!--Nomenclature_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
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{{BacktoTop}} | {{BacktoTop}} | ||
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<big>'''Gene Ontology'''</big> | <big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.21293.H46aa42291caff--> | ||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="H46aa42291caff" class=" tableEdit GO_table_product dataTable" | ||
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!|Qualifier!!GO ID!!GO term name!!Reference | !|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status | ||
|- | |- | ||
| | | | ||
| | | | ||
GO:0003978 | |||
| | | | ||
UDP- | UDP-glucose 4-epimerase activity | ||
| | | | ||
GOA:interpro<br />GO_REF:0000002 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
| Line 93: | Line 96: | ||
F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0003978 | |||
| | | | ||
UDP- | UDP-glucose 4-epimerase activity | ||
| | | | ||
GOA:spec<br />GO_REF:0000003 | |||
| | | | ||
IEA: Inferred from Electronic Annotation | IEA: Inferred from Electronic Annotation | ||
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F | F | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0005829 | |||
| | | | ||
cytosol | |||
| | | | ||
PMID:16858726 | |||
| | | | ||
IDA: Inferred from Direct Assay | |||
| | | | ||
| | | | ||
C | |||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0006012 | |||
| | | | ||
galactose metabolic process | |||
| | | | ||
PMID:13914787 | |||
| | | | ||
IMP: Inferred from Mutant Phenotype | |||
| | | | ||
| | | | ||
P | P | ||
| | | | ||
Seeded from EcoCyc | Seeded from [[EcoCyc]] (v14.0) | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0006012 | |||
| | | | ||
galactose metabolic process | |||
| | | | ||
PMID:7025059 | |||
| | | | ||
IMP: Inferred from Mutant Phenotype | |||
| | | | ||
| | | | ||
P | |||
| | | | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0005975 | |||
| | | | ||
carbohydrate metabolic process | |||
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PMID:7025059 | |||
| | | | ||
IMP: Inferred from Mutant Phenotype | |||
| | | | ||
| | | | ||
P | P | ||
| | | | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0016052 | |||
| | | | ||
carbohydrate catabolic process | |||
| | | | ||
PMID:7025059 | |||
| | | | ||
IGI: Inferred from Genetic Interaction | |||
| | | | ||
| | | | ||
P | |||
| | | | ||
| | | | ||
Missing: with/from | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0016857 | |||
| | | | ||
racemase and epimerase activity, acting on carbohydrates and derivatives | |||
| | | | ||
PMID:1821806 | |||
| | | | ||
IDA: Inferred from Direct Assay | |||
| | | | ||
| | | | ||
F | |||
| | | | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0006012 | |||
| | | | ||
galactose metabolic process | |||
| | | | ||
PMID:1821806 | |||
| | | | ||
IDA: Inferred from Direct Assay | |||
| | | | ||
| | | | ||
P | P | ||
| | | | ||
| | | | ||
complete | |||
|- | |- | ||
| | | | ||
| | | | ||
GO:0005737 | |||
| | | | ||
cytoplasm | |||
| | | | ||
PMID:1821806 | |||
| | | | ||
IDA: Inferred from Direct Assay | |||
| | | | ||
| | | | ||
C | |||
| | | | ||
| | | | ||
complete | |||
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<span id="Interactions"></span><big>'''Interactions'''</big> | |||
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!|Partner Type!!Partner!!Notes!!References!!Evidence | |||
|- | |- | ||
| | | | ||
Protein | |||
| | | | ||
[[Category: | Subunits of [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]] | ||
| | | | ||
could be indirect | |||
| | | | ||
| | | | ||
|- | |- | ||
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Protein | |||
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rsuA<br /> | |||
| | | | ||
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PMID:16606699 | |||
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Experiment(s):EBI-1137880<br /> | |||
|- | |- | ||
| | | | ||
Protein | |||
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yfcH<br /> | |||
| | | | ||
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PMID:16606699 | |||
| | | | ||
Experiment(s):EBI-1137880<br /> | |||
|- | |- | ||
| | | | ||
Protein | |||
| | | | ||
nadE<br /> | |||
| | | | ||
| | | | ||
PMID:16606699 | |||
| | | | ||
Experiment(s):EBI-1137880<br /> | |||
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===Notes=== | ===Notes=== | ||
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!Compartment!!Description!!Evidence!!Notes | !|Compartment!!Description!!Evidence!!Reference/Source!!Notes | ||
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{|{{ | {| id="T46aa426d54e5c" class=" tableEdit Product_phys_prop_table" | ||
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!align=left {{table heading style}} |Name | |||
|| | |||
|- | |- | ||
!{{table heading style}} |Sequence | !align=left {{table heading style}} |Sequence | ||
| | || | ||
[http://biocyc.org/ECOLI/sequence-aa?object=UDPGLUCEPIM-MONOMER at EcoCyc] | [http://biocyc.org/ECOLI/sequence-aa?object=UDPGLUCEPIM-MONOMER at EcoCyc] | ||
<pre>MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR | <pre>MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR | ||
| Line 459: | Line 391: | ||
ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD</pre> | ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD</pre> | ||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Length | ||
| | || | ||
338 | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |Mol. Wt | ||
| | || | ||
37.264 kDa | |||
|- | |- | ||
!{{table heading style}} | | !align=left {{table heading style}} |pI | ||
| | || | ||
6.3 (calculated) | |||
|- | |- | ||
| | !align=left {{table heading style}} |Extinction coefficient | ||
|| | |||
48,360 - 48,735 (calc based on 14 Y, 5 W, and 3 C residues) | |||
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<span id=" | <span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table> | ||
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! | !|Type!!Residues!!Description!!Notes!!References | ||
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| | | | ||
Domain | |||
| | | | ||
3..262 | |||
| | | | ||
[http:// | [http://pfam.janelia.org/family/PF01370 PF01370 NAD dependent epimerase/dehydratase family] | ||
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PMID:19920124 | |||
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<span id='Structure'></span><big>'''Structure'''</big><br /> | |||
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| | !align=left {{table heading style}} |Structures | ||
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<beststructure> | |||
| | gene=galE | ||
taxon=562,83333 | |||
</beststructure> | |||
|- | |- | ||
!align=left {{table heading style}} |Models | |||
|| | |||
View models at: | |||
* [http://www.proteinmodelportal.org/query/uniprot/P09147 Protein Model Portal] | |||
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P09147 PSI-PRED (P09147)] | |||
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P09147 Genomes TO Protein (P09147)] (GTOP) | |||
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==== Structure figures ==== | |||
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===Notes=== | ===Notes=== | ||
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===Notes=== | ===Notes=== | ||
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!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
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| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/49176045 GI:49176045] | |||
| | |||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]]) | |||
| | |||
[http://www.ncbi.nlm.nih.gov/protein/NP_415280.3 GeneID:945354] | |||
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Escherichia coli str. K-12 substr. MG1655 | |||
|- | |||
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[[ASAP]] | |||
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ASAP:ABE-0002575 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
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[http:// | [http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]]) | ||
| | | | ||
[http:// | [http://www.uniprot.org/uniprot/P09147 UniProtKB/Swiss-Prot:P09147] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EcoCyc]] | |||
| | | | ||
EcoCyc:EG10362 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[EcoGene]] | |||
| | | | ||
EcoGene:EG10362 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http://www.ncbi.nlm.nih.gov/ | [http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]]) | ||
| | | | ||
[http://www.ncbi.nlm.nih.gov/ | [http://www.ncbi.nlm.nih.gov/gene?term=945354 GeneID:945354] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000355 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[EchoBASE]] | |||
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EchoBASE:EB0357 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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[[Category:Proteins]] | [[Category:Proteins]] | ||
[[Category: | [[Category:RefGenome_Annotated_Gene]] | ||
Latest revision as of 20:29, 17 February 2015
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
GalE |
|---|---|
| Synonyms |
UDP-galactose-4-epimerase[1], B0759[2][1], GalD[2][1], GalE[2][1] , ECK0748, galD, JW0742, b0759 |
| Product description |
UDP-glucose 4-epimerase[2][3]; Component of UDP-glucose 4-epimerase[2][3] UDP-galactose 4-epimerase; hexose-1-phosphate uridylyltransferase[4] |
| EC number (for enzymes) | |
| edit table |
Notes
Function
Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003978 |
UDP-glucose 4-epimerase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003978 |
UDP-glucose 4-epimerase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006012 |
galactose metabolic process |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006012 |
galactose metabolic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0005975 |
carbohydrate metabolic process |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
| GO:0016052 |
carbohydrate catabolic process |
IGI: Inferred from Genetic Interaction |
P |
Missing: with/from | ||||
| GO:0016857 |
racemase and epimerase activity, acting on carbohydrates and derivatives |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006012 |
galactose metabolic process |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of UDP-glucose 4-epimerase |
could be indirect |
||
|
Protein |
rsuA |
Experiment(s):EBI-1137880 | ||
|
Protein |
yfcH |
Experiment(s):EBI-1137880 | ||
|
Protein |
nadE |
Experiment(s):EBI-1137880 | ||
| edit table |
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
Notes
Structure and Physical Properties
Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR NEALMTEILH DHAIDTVIHF AGLKAVGESV QKPLEYYDNN VNGTLRLISA MRAANVKNFI FSSSATVYGD QPKIPYVESF PTGTPQSPYG KSKLMVEQIL TDLQKAQPDW SIALLRYFNP VGAHPSGDMG EDPQGIPNNL MPYIAQVAVG RRDSLAIFGN DYPTEDGTGV RDYIHVMDLA DGHVVAMEKL ANKPGVHIYN LGAGVGNSVL DVVNAFSKAC GKPVNYHFAP RREGDLPAYW ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD |
| Length |
338 |
| Mol. Wt |
37.264 kDa |
| pI |
6.3 (calculated) |
| Extinction coefficient |
48,360 - 48,735 (calc based on 14 Y, 5 W, and 3 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
|
Structure figures
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ SOFFER, RL (1961) Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J. Bacteriol. 82 471-8 PubMed
- ↑ 7.0 7.1 7.2 Fietta, A et al. (1981) R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE Mutants. Plasmid 6 78-85 PubMed
- ↑ 8.0 8.1 8.2 Vorgias, CE et al. () Overexpression and purification of the galactose operon enzymes from Escherichia coli. Protein Expr. Purif. 2 330-8 PubMed
- ↑ 9.0 9.1 9.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003978 ! UDP-glucose 4-epimerase activity
- GO:0005829 ! cytosol
- GO:0006012 ! galactose metabolic process
- GO:0005975 ! carbohydrate metabolic process
- GO:0016052 ! carbohydrate catabolic process
- GO:0016857 ! racemase and epimerase activity, acting on carbohydrates and derivatives
- GO:0005737 ! cytoplasm
- Proteins
- RefGenome Annotated Gene