galE:Gene Product(s): Difference between revisions

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|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Localization |Localization]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Sequence|Sequence]]
|{{Sub}}[[#Domains/Motifs/Modification Sites|Domains]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Structure|Structure]]
|{{Sub}}[[#Gene Product Resources|Resources]]
|{{Sub}}[[#Gene Product Resources|Resources]]
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{|  id="E46aa4227b97b9"  class=" tableEdit Product_nomenclature_table" 
 
|-
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!{{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
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GalE product
GalE  
|-
|-
!{{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
|
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UDP-galactose-4-epimerase product<ref name='LIB:Riley_2006'/>, B0759 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalD product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalE product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
''UDP-galactose-4-epimerase<ref name='LIB:Riley_2006'/>, B0759<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalD<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, GalE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/> , ECK0748, galD, JW0742, b0759''
|-
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!{{table heading style}} |Product description
!align=left {{table heading style}} |Product description
|
||
UDP-glucose 4-epimerase monomer<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
UDP-glucose 4-epimerase<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  


Component of ; [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
Component of [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]]<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>


UDP-galactose 4-epimerase; hexose-1-phosphate uridylyltransferase<ref name='LIB:EcoGene'/>
|-
|-
!{{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
|
||
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.2 5.1.3.2]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.1.3.2 5.1.3.2]<ref name='LIB:Riley_2006'/>
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<protect><!--Nomenclature_notes--></protect>
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===Notes===
===Notes===
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{{BacktoTop}}
{{BacktoTop}}
<protect>
<protect>
<big>'''Gene Ontology'''</big>
<big>'''Gene Ontology'''</big><br /><!--box uid=421aa90e079fa326b6494f812ad13e79.21293.H46aa42291caff-->
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="H46aa42291caff"  class=" tableEdit GO_table_product dataTable"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-  
|-  
|
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|
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[[Category:GO:0003978_!_UDP-glucose_4-epimerase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003978_!_UDP-glucose_4-epimerase_activity GO:0003978]
GO:0003978
|
|
UDP-glucose_4-epimerase_activity
UDP-glucose 4-epimerase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002]<br />
GOA:interpro<br />GO_REF:0000002
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0003978_!_UDP-glucose_4-epimerase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003978_!_UDP-glucose_4-epimerase_activity GO:0003978]
GO:0003978
|
|
UDP-glucose_4-epimerase_activity
UDP-glucose 4-epimerase activity
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000003 GO_REF:0000003]<br />
GOA:spec<br />GO_REF:0000003
|
|
IEA: Inferred from Electronic Annotation
IEA: Inferred from Electronic Annotation
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F
F
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0005975_!_carbohydrate_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005975_!_carbohydrate_metabolic_process GO:0005975]
GO:0005829
|
|
carbohydrate_metabolism
cytosol
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004]<br />
PMID:16858726
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
|
SP_KW:KW-0119
 
|
|
P
C
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0006012_!_galactose_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006012_!_galactose_metabolic_process GO:0006012]
GO:0006012
|
|
galactose_metabolism
galactose metabolic process
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004]<br />
PMID:13914787
|
|
IEA: Inferred from Electronic Annotation
IMP: Inferred from Mutant Phenotype
|
|
SP_KW:KW-0299
 
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from [[EcoCyc]] (v14.0)
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0016853_!_isomerase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016853_!_isomerase_activity GO:0016853]
GO:0006012
|
|
isomerase_activity
galactose metabolic process
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000004 GO_REF:0000004]<br />
PMID:7025059
|
|
IEA: Inferred from Electronic Annotation
IMP: Inferred from Mutant Phenotype
|
|
SP_KW:KW-0413
 
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0044237_!_cellular_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0044237_!_cellular_metabolic_process GO:0044237]
GO:0005975
|
|
cellular_metabolism
carbohydrate metabolic process
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002]<br />
PMID:7025059
|
|
IEA: Inferred from Electronic Annotation
IMP: Inferred from Mutant Phenotype
|
|
InterPro:IPR001509
 
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete
|-  
|-  
|
|


|
|
[[Category:GO:0003824_!_catalytic_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003824_!_catalytic_activity GO:0003824]
GO:0016052
|
|
catalytic_activity
carbohydrate catabolic process
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002]<br />
PMID:7025059
|
|
IEA: Inferred from Electronic Annotation
IGI: Inferred from Genetic Interaction
|
|
InterPro:IPR001509
 
|
|
F
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
Missing: with/from
|-  
|-  
|
|
 
|
|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
GO:0016857
|
|
cytoplasm
racemase and epimerase activity, acting on carbohydrates and derivatives
|
|
PMID:1821806
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
C
F
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing 
complete
|-  
|-  
|
|


|
|
[[Category:GO:0006012_!_galactose_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006012_!_galactose_metabolic_process GO:0006012]
GO:0006012
|
|
galactose_metabolism
galactose metabolic process
|
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002]<br />
PMID:1821806
|
|
IEA: Inferred from Electronic Annotation
IDA: Inferred from Direct Assay
|
|
InterPro:IPR005886
 
|
|
P
P
|
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
 
|
|
complete
|-  
|-  
|
|
 
|
|
[[Category:GO:0009242_!_colanic_acid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009242_!_colanic_acid_biosynthetic_process GO:0009242]
GO:0005737
|
|
colanic acid biosynthetic process
cytoplasm
|
|
PMID:1821806
|
|
IDA: Inferred from Direct Assay
|
|
 
|
|
P
C
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
 
|
|
required fields missing  
complete
 
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<span id="Interactions"></span><big>'''Interactions'''</big>
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!|Partner Type!!Partner!!Notes!!References!!Evidence
|-  
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|
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Protein
|
|
[[Category:GO:0016052_!_carbohydrate_catabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016052_!_carbohydrate_catabolic_process GO:0016052]  
Subunits of [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]]  
|
|
carbohydrate catabolic process
could be indirect
|
|
 
|
|
   
   
|
|
P
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing 
|-  
|-  
|
|
Protein
|
|
[[Category:GO:0009435_!_NAD_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009435_!_NAD_biosynthetic_process GO:0009435]
rsuA<br />
|
|
NAD biosynthetic process
 
|
|
PMID:16606699
|
|
|
|
P
Experiment(s):EBI-1137880<br />  
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing 
|-  
|-  
|
|
Protein
|
|
[[Category:GO:0009269_!_response_to_desiccation]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009269_!_response_to_desiccation GO:0009269]
yfcH<br />
|
|
response to desiccation
 
|
|
|
|
|
P
PMID:16606699
|
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
Experiment(s):EBI-1137880<br />  
|
required fields missing 
|-  
|-  
|
|
Protein
|
[[Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0042280_!_cell_surface_antigen_activity,_host-interacting GO:0042280]
|
cell surface antigen activity, host-interacting
|
|
|
|
F
|
Seeded from Riley et  al 2006 <ref name='LIB:Riley_2006'/>.
|
required fields missing 
|-
|
|
[[Category:GO:0030113_!_capsule_(sensu_Bacteria)]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0030113_!_capsule_(sensu_Bacteria) GO:0030113]
|
|
capsule (sensu Bacteria)
nadE<br />
|
|
|
|
C
|
Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
|
required fields missing 
|-
|
|


|
|
[[Category:GO:0050662_!_coenzyme_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0050662_!_coenzyme_binding GO:0050662]
PMID:16606699
|
|
coenzyme_binding
Experiment(s):EBI-1137880<br />  
|
[http://www.geneontology.org/cgi-bin/references.cgi/#GO_REF:0000002 GO_REF:0000002]<br />
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR001509
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|


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<span id="Interactions"></span><big>'''Interactions'''</big>
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!Partner Type!!Partner!!Notes
|-
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Protein
|
Subunits of [[:Category:Complex:UDP-glucose 4-epimerase|UDP-glucose 4-epimerase]]
|
could be indirect 
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<!--Function_notes--></protect>
===Notes===
===Notes===
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!Compartment!!Description!!Evidence!!Notes
!|Compartment!!Description!!Evidence!!Reference/Source!!Notes
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{|{{Prettytable}}
{|   id="T46aa426d54e5c"  class=" tableEdit Product_phys_prop_table" 
 
|-
!align=left {{table heading style}} |Name
||
 
|-
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!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
|
||
[http://biocyc.org/ECOLI/sequence-aa?object=UDPGLUCEPIM-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=UDPGLUCEPIM-MONOMER at EcoCyc]  
<pre>MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR
<pre>MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR
Line 459: Line 391:
ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD</pre>  
ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD</pre>  
|-
|-
!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Length
|
||
37.27 kDa (calc) <ref name='LIB:EcoCyc10.6'/>
338
|-
|-
!{{table heading style}} |pI
!align=left {{table heading style}} |Mol. Wt
|
||
6.28<ref name='LIB:EcoCyc10.6'/>
37.264 kDa
|-
|-
!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |pI
|
||
48360 - 48735 (calc based on 14 Y, 5 W, and 3 C residues)  
6.3 (calculated)
|-
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!align=left {{table heading style}} |Extinction coefficient
||
48,360 - 48,735 (calc based on 14 Y, 5 W, and 3 C residues)
 
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<big>'''Domains/Motifs/Modification Sites'''</big>
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!Type!!Residues!!Description!!Notes
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<span id="Structure"></span><big>'''Structure'''</big>
<span id="Domains/Motifs/Modification Sites"><big>'''Domains/Motifs/Modification Sites'''</big></span><table>
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|- {{table heading style}}
|- {{table heading style}}
!Structure Type!!Organism!!Accession!!Residues covered!!Notes
!|Type!!Residues!!Description!!Notes!!References
|-
|-  
|
|
X-RAY DIFFRACTION
Domain
|
|
Escherichia coli
3..262
|
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2UDP 2UDP] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
[http://pfam.janelia.org/family/PF01370 PF01370  NAD dependent epimerase/dehydratase family]
|
|
 
|
|
 
PMID:19920124
|-
 
|
|- class="tableEdit_footer"
X-RAY DIFFRACTION
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.21293.D46aa426db0285&page=21293&pagename={{FULLPAGENAMEE}}&type=&template=Product_motif_table edit table]</span> || || || ||
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<motif_map/>
Escherichia coli
</td>
</tr>
|
</table>
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1XEL 1XEL] UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI
 
|
<table><tr valign='top'><td>
   
<span id='Structure'></span><big>'''Structure'''</big><br />
|
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|-
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!align=left {{table heading style}} |Structures
X-RAY DIFFRACTION
||
<beststructure>
|
gene=galE
Escherichia coli
taxon=562,83333
</beststructure>
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1UDC 1UDC] STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-MANNOSE
|
|
 
|-
|-
|
!align=left {{table heading style}} |Models
X-RAY DIFFRACTION
||
View models at:
|
* [http://www.proteinmodelportal.org/query/uniprot/P09147 Protein Model Portal]
Escherichia coli
* [http://bioinf.cs.ucl.ac.uk/genome3d/read?uniprot_id=P09147 PSI-PRED (P09147)]
* [http://spock.genes.nig.ac.jp/~genome/cgi-bin/gkeytext.pl.cgi?A&QUERY1=P09147 Genomes TO Protein (P09147)] (GTOP)
|
 
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1UDB 1UDB] STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-4-DEOXY-4-FLUORO-ALPHA-D-GLUCOSE
|- class="tableEdit_footer"
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.21293.X46aa426e07634&page=21293&pagename={{FULLPAGENAMEE}}&type=1&template=Product_structure_table edit table]</span> ||
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1UDA 1UDA] STRUCTURE OF UDP-GALACTOSE-4-EPIMERASE COMPLEXED WITH UDP-4-DEOXY-4-FLUORO-ALPHA-D-GALACTOSE
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NAI 1NAI] UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI, OXIDIZED
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1NAH 1NAH] UDP-GALACTOSE 4-EPIMERASE FROM ESCHERICHIA COLI, REDUCED
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LRL 1LRL] Crystal Structure of UDP-Galactose 4-Epimerase Mutant Y299C Complexed with UDP-Glucose
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LRK 1LRK] Crystal Structure of Escherichia coli UDP-Galactose 4-Epimerase Mutant Y299C Complexed with UDP-N-acetylglucosamine
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1LRJ 1LRJ] Crystal Structure of E. coli UDP-Galactose 4-Epimerase Complexed with UDP-N-Acetylglucosamine
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KVU 1KVU] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KVT 1KVT] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KVS 1KVS] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KVR 1KVR] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1KVQ 1KVQ] UDP-GALACTOSE 4-EPIMERASE COMPLEXED WITH UDP-PHENOL
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A9Z 1A9Z] UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GALACTOSE
|
|
 
|-
|
X-RAY DIFFRACTION
|
Escherichia coli
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A9Y 1A9Y] UDP-GALACTOSE 4-EPIMERASE MUTANT S124A/Y149F COMPLEXED WITH UDP-GLUCOSE
|
|
 
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==== Structure figures ====
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
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===Notes===
===Notes===


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===Notes===
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|- {{table_heading_style}}
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/49176045 GI:49176045]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[http://www.ncbi.nlm.nih.gov/protein NCBI (Protein)] ([[NCBI (Protein)|EcoliWiki Page]])
|
[http://www.ncbi.nlm.nih.gov/protein/NP_415280.3 GeneID:945354]
|
Escherichia coli str. K-12 substr. MG1655
|-
|
[[ASAP]]
|
ASAP:ABE-0002575
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://www.pir.uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=UDPGLUCEPIM-MONOMER EcoCyc:UDPGLUCEPIM-MONOMER]
[http://www.uniprot.org/uniprot/P09147 UniProtKB/Swiss-Prot:P09147]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])
[[EcoCyc]]
|
|
[[uniprot:P09147|UniProt:P09147]] 
EcoCyc:EG10362
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://pfam.sanger.ac.uk/family?acc=PF01370 PFAM:PF01370] 
EcoGene:EG10362
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI (Gene)] ([[NCBI (Gene)|EcoliWiki Page]])
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_415280 RefSeq:NP_415280]
[http://www.ncbi.nlm.nih.gov/gene?term=945354 GeneID:945354]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P09147 ModBase:P09147] 
RegulonDB:ECK120000355
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://www.rcsb.org/pdb PDB] ([[PDB|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=1A9Y PDB:1A9Y] 
EchoBASE:EB0357
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</noinclude>
===Categories===
===Categories===
{{BacktoTop}}
{{BacktoTop}}
[[Category:Proteins]]
[[Category:Proteins]]
[[Category:GO:0005737_!_cytoplasm]][[Category:GO:0006012_!_galactose metabolic process]][[Category:GO:0009242_!_colanic acid biosynthetic process]][[Category:GO:0016052_!_carbohydrate catabolic process]][[Category:GO:0009435_!_NAD biosynthetic process]][[Category:GO:0009269_!_response to desiccation]][[Category:GO:0042280_!_cell surface antigen activity, host-interacting]][[Category:GO:0030113_!_capsule (sensu Bacteria)]][[Category:Complex:UDP-glucose 4-epimerase]][[Category:Products with structures in PDB]]
[[Category:RefGenome_Annotated_Gene]]

Latest revision as of 20:29, 17 February 2015

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GalE

Synonyms

UDP-galactose-4-epimerase[1], B0759[2][1], GalD[2][1], GalE[2][1] , ECK0748, galD, JW0742, b0759

Product description

UDP-glucose 4-epimerase[2][3];

Component of UDP-glucose 4-epimerase[2][3]

UDP-galactose 4-epimerase; hexose-1-phosphate uridylyltransferase[4]

EC number (for enzymes)


Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003978

UDP-glucose 4-epimerase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005886

F

Seeded from EcoCyc (v14.0)

complete

GO:0003978

UDP-glucose 4-epimerase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:5.1.3.2

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006012

galactose metabolic process

PMID:13914787[6]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006012

galactose metabolic process

PMID:7025059[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005975

carbohydrate metabolic process

PMID:7025059[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016052

carbohydrate catabolic process

PMID:7025059[7]

IGI: Inferred from Genetic Interaction

P

Missing: with/from

GO:0016857

racemase and epimerase activity, acting on carbohydrates and derivatives

PMID:1821806[8]

IDA: Inferred from Direct Assay

F

complete

GO:0006012

galactose metabolic process

PMID:1821806[8]

IDA: Inferred from Direct Assay

P

complete

GO:0005737

cytoplasm

PMID:1821806[8]

IDA: Inferred from Direct Assay

C

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of UDP-glucose 4-epimerase

could be indirect

Protein

rsuA

PMID:16606699[9]

Experiment(s):EBI-1137880

Protein

yfcH

PMID:16606699[9]

Experiment(s):EBI-1137880

Protein

nadE

PMID:16606699[9]

Experiment(s):EBI-1137880


Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes


Notes

Structure and Physical Properties

[back to top]


Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR
NEALMTEILH DHAIDTVIHF AGLKAVGESV QKPLEYYDNN VNGTLRLISA MRAANVKNFI
FSSSATVYGD QPKIPYVESF PTGTPQSPYG KSKLMVEQIL TDLQKAQPDW SIALLRYFNP
VGAHPSGDMG EDPQGIPNNL MPYIAQVAVG RRDSLAIFGN DYPTEDGTGV RDYIHVMDLA
DGHVVAMEKL ANKPGVHIYN LGAGVGNSVL DVVNAFSKAC GKPVNYHFAP RREGDLPAYW
ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD
Length

338

Mol. Wt

37.264 kDa

pI

6.3 (calculated)

Extinction coefficient

48,360 - 48,735 (calc based on 14 Y, 5 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..262

PF01370 NAD dependent epimerase/dehydratase family

PMID:19920124[10]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=galE taxon=562,83333 </beststructure>

Models

View models at:

Structure figures

Notes

Gene Product Resources

[back to top]


See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference


Notes

Accessions in Other Databases

[back to top]


See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176045

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945354

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002575

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P09147

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10362

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10362

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945354

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000355

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0357

Escherichia coli str. K-12 substr. MG1655


Notes

Links

[back to top]




References

[back to top]


See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ SOFFER, RL (1961) Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J. Bacteriol. 82 471-8 PubMed
  7. ↑ 7.0 7.1 7.2 Fietta, A et al. (1981) R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE Mutants. Plasmid 6 78-85 PubMed
  8. ↑ 8.0 8.1 8.2 Vorgias, CE et al. () Overexpression and purification of the galactose operon enzymes from Escherichia coli. Protein Expr. Purif. 2 330-8 PubMed
  9. ↑ 9.0 9.1 9.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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