metA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="L46aa04bbaa76c"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''metA''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''metA''
Methionine
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK4005, b4013, JW3973''<ref name='LIB:Riley_2006'/>, JW3973''


|-
|- class="tableEdit_footer"
!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18585.L46aa04bbaa76c&page=18585&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
|
''ECK4005, b4013, JW3973''<ref name='LIB:Riley_2006'/>
|-
|colspan='2'|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18585.L46aa04bbaa76c&page=18585&pagename={{FULLPAGENAMEE}} edit table]
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='148965'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="N46aa04bc3949b"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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90.79 minutes, 90.79 minutes&nbsp;
90.79 minutes, 90.79 minutes&nbsp;
|
|
MG1655: 4212303..4213232<br />{{GBrowseFigureByCoord|4211303|4214232|ecoli|MG1655}}
MG1655: 4212303..4213232<br /><gbrowseImage>
name=NC_000913:4212303..4213232
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10581 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10581 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|4211303|4214232|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|4211303|4214232|GBrowse(MG1655)}}
   
   
|
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{{GBrowseFastaLink|ecoli|NC_000913|4212303|4213232|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|4212303|4213232|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10581 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10581 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 4193725..4194654<br /><gbrowseImage>
name=NC_012967:4193725..4194654
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|4193725|4194654|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4193725|4194654|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 4101983..4102912<br /><gbrowseImage>
name=NC_012759:4101983..4102912
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|4101983|4102912|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4101983|4102912|FASTA Dump}}
|-  
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W3110
W3110
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&nbsp;
&nbsp;
|
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W3110: 4217870..4218799<br />{{GBrowseFigureByCoord|4216870|4219799|ecoli_W3110|W3110}}  
W3110: 4217870..4218799<br /><gbrowseImage>
name=NC_007779:4217870..4218799
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|4216870|4219799|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4217870|4218799|FASTA Dump}}  
|-
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[[DH10B]]
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|4216870|4219799|GBrowse(W3110)}}
 
|
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|4217870|4218799|FASTA Dump}}  
DH10B: 4311999..4312928<br /><gbrowseImage>
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name=NC_010473:4311999..4312928
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source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|4310141|4314786|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|4310141|4314786|FASTA Dump}}  
 
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18585.N46aa04bc3949b&page=18585&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table]</span> || || || ||
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="W46aa04bd4b7bd"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109137 CGSC10856]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109137 CGSC10856]<ref name='LIB:cgsc'/>  
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|
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[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
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[[cgscmutation:101824|CGSC:101824]]
[[cgscmutation:101824|CGSC:101824]]
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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18585.W46aa04bd4b7bd&page=18585&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || ||
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.18585.L4e04bf196b4d6-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="L4e04bf196b4d6"  class=" tableEdit Genetic_interaction_table dataTable" 
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===Notes===


==Genetic Resources==
==Genetic Resources==
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JW3973
JW3973
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
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[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCATCCAGCGTTGGATTCATGTG  
Primer 2:CCATCCAGCGTTGGATTCATGTG  
|-
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[[thiC:Gene|thiC39::Tn10]]
[[thiC:Gene|thiC39::Tn10]]
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CAG18500 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40361 CGSC7472]<ref name='LIB:cgsc'/>
CAG18500 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40361 CGSC7472]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 52% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 52% <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:thi-39::Tn10  
Synonyms:thi-39::Tn10  
|-
|-  
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[[malF:Gene|malF3089::Tn10]]
[[malF:Gene|malF3089::Tn10]]
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est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>  
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===Notes===
===Notes===
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="B46aa04beb40a7"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10581 EcoCyc:EG10581]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0576 EchoBASE:EB0576]
|
|-
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
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[http://chase.ou.edu/macro/searchlink.php?bnum=b4013 EcoliGenExpDB:b4013]
EcoCyc:EG10581
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C4970 ECOL199310CYC:C4970]
EcoGene:EG10581
|
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10581 EcoGene:EG10581]
RegulonDB:ECK120000574
|
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=G7E-0 ECOO157CYC:G7E-0]
[http://www.ncbi.nlm.nih.gov/gene?term=948513 GeneID:948513]
|
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Escherichia coli str. K-12 substr. MG1655
|-  
|-  
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/516 CGSC:516]
EchoBASE:EB0576
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0013121&formSubmitReturn=1 ASAP:ABE-0013121] 
ASAP:ABE-0013121
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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===Notes===
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<noinclude><protect>
<noinclude><protect>
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{{BacktoTop}}
{{BacktoTop}}
{{RefHelp}}
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</protect></noinclude>
===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:met genes]]
[[Category:met genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:50, 19 September 2013

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metA

Mnemonic

Methionine

Synonyms

ECK4005, b4013, JW3973[1], JW3973

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.79 minutes, 90.79 minutes 

MG1655: 4212303..4213232
<gbrowseImage> name=NC_000913:4212303..4213232 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4193725..4194654
<gbrowseImage> name=NC_012967:4193725..4194654 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4101983..4102912
<gbrowseImage> name=NC_012759:4101983..4102912 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4217870..4218799
<gbrowseImage> name=NC_007779:4217870..4218799 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4311999..4312928
<gbrowseImage> name=NC_010473:4311999..4312928 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmetA (Keio:JW3973)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10856[3]

metA28

CGSC:7080

metA115

CGSC:8439

metA160

CGSC:10344

ΔmetA780::kan

PMID:16738554[2]

CGSC:101824


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3973

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCGATTCGTGTGCCGGACGA

Primer 2:CCATCCAGCGTTGGATTCATGTG

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 52% [5]
Synonyms:thi-39::Tn10

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[3]

est. P1 cotransduction: 33% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10581

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10581

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000574

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948513

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0576

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013121

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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