argA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|{{Sub}}[[Talk:{{PAGENAMEE}}| Suggestions]]
|}
|}
[[Category:Gene List:MG1655]][[Category:Gene List:W3110]][[Category:Genes in the Keio knockout collection]][[Category:Genes in the ASKA clone collection]]
</noinclude><protect>
</noinclude><protect>
==Nomenclature==
==Nomenclature==
{{BacktoTop}}
{{BacktoTop}}
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{|  id="M46a9e6febe054"  class=" tableEdit Gene_nomenclature_table" 
 
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
|
||
''argA''  
''argA''
|-
!align=left {{table_heading_style}} |Mnemonic
||
Arginine
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK2814, b2818, JW2786, Arg1, Arg2''<ref name='LIB:Riley_2006'/>, Arg2, argB''


|-
|- class="tableEdit_footer"
!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.16203.M46a9e6febe054&page=16203&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK2814, b2818, JW2786, Arg1, Arg2''<ref name='LIB:Riley_2006'/>
|-
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===Notes===
===Notes===
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<protect>
<protect>
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46a9e6ff48a2a"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
Line 63: Line 76:
63.52 minutes&nbsp;
63.52 minutes&nbsp;
|
|
MG1655: 2947264..2948595<br />{{GBrowseFigureByCoord|2946264|2949595|ecoli|MG1655}}
MG1655: 2947264..2948595<br /><gbrowseImage>
name=NC_000913:2947264..2948595
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10063 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10063 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:2946264..2949595 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|2946264|2949595|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:2947264..2948595&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|2947264|2948595|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10063 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10063 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 2843861..2845192<br /><gbrowseImage>
name=NC_012967:2843861..2845192
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|2843861|2845192|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|2843861|2845192|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 2834412..2835743<br /><gbrowseImage>
name=NC_012759:2834412..2835743
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|2834412|2835743|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|2834412|2835743|FASTA Dump}}
|-  
|
|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 2947898..2949229<br />{{GBrowseFigureByCoord|2946898|2950229|ecoli_W3110|W3110}}  
W3110: 2947898..2949229<br /><gbrowseImage>
name=NC_007779:2947898..2949229
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|2946898|2950229|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|2947898|2949229|FASTA Dump}}  
|-
|
[[DH10B]]
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:2946898..2950229 GBrowse(W3110)]
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:2947898..2949229&plugin_action=Go FASTA Dump]
DH10B: 3041134..3042465<br /><gbrowseImage>
|-class='sortbottom'
name=NC_010473:3041134..3042465
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source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|DH10B|NC_010473|3038472|3045127|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|3038472|3045127|FASTA Dump}}
 
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===Notes===
===Notes===
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|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-class='sortbottom'
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Coding Start (SO:0000323)
|
[[MG1655]]
|
2947267
|
Edman degradation
|
PMID:3320971<br />
|
 
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<protect><!--Sequence_notes--></protect>
<protect><!--Sequence_notes--></protect>
====Notes====
====Notes====
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="I46a9e7004b9db"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-
|-
|
argAR58H
|
 
|
R58H
|
Auxotrophies
|
In EE11
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-
|
argAH15Y
|
 
|
H15Y
|
Auxotrophies
|
In EE17
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-
|
argAY19C
|
 
|
Y19C
|
Auxotrophies
|
In EE51
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-
|
argAG287S
|
 
|
G287S
|
Auxotrophies
|
In PT2M216
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-
|
argAQ432R
|
 
|
Q432R
|
Auxotrophies
|
In PT2M217
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-
|
argAS54N
|
 
|
S54N
|
Auxotrophies
|
In PT2M217
|
 
|
 
|
seeded from [http://www.uniprot.org/uniprot/P0A6C5 UniProt:P0A6C5]
|-  
|
|
&Delta;argA (Keio:JW2786)
ΔargA (Keio:JW2786)
|
|
deletion
deletion
|
|
deletion
deletion
|
Auxotrophies
|
|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
[http://cgsc2.biology.yale.edu/Strain.php?ID=108462 CGSC10181]<ref name='LIB:cgsc'/>
|
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN-I-SceI] (FB20943)
|
Insertion at nt 933 in Plus orientation
|
|
Auxotrophies
|
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20943]
|
contains [[pKD46]]
|-
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN-I-SceI] (FB20944)
|
Insertion at nt 933 in Plus orientation
|
|
Auxotrophies
|
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20944]
|
does not contain [[pKD46]]
|-
|
''argA21''
|
|
|
Auxotrophies
|
|
|
[[cgscmutation:5137|CGSC:5137]]
|
|-
|
''argA42''
|
|
|
Auxotrophies
|
|
|
[[cgscmutation:5157|CGSC:5157]]
|
|-
|
''argA52''
|
|
|
Auxotrophies
|
|
|
[[cgscmutation:5167|CGSC:5167]]
|
|-
|
''argA76''(Fs)
|
|
|
Auxotrophies
|
|
|
[[cgscmutation:5192|CGSC:5192]]
|
frameshift mutation
|-
|
''argA103''
|
|


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|
|
[[PMID:1673855]]<ref name=PMID:1673855/>
Auxotrophies
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]  
 
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|
[[cgscmutation:5207|CGSC:5207]]
|
|-
|
''argA22''
|
 
|
 
|
Auxotrophies
|
 
|
 
|
[[cgscmutation:6000|CGSC:6000]]
|
|-
|
''argA81::Tn10''
|
 
|
 
|
Auxotrophies
|
 
|
 
|
[[cgscmutation:7098|CGSC:7098]]
|
|-
|
''argA9999''
|
 
|
 
|
Auxotrophies
|
 
|
 
|
[[cgscmutation:9194|CGSC:9194]]
|
|-
|
''ΔargA743::kan''
|
deletion
|
deletion
|
Auxotrophies
|
 
|
PMID:16738554
|
[[cgscmutation:100036|CGSC:100036]]
|
 
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="A4e04b77dd16f0"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
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===Notes===


==Genetic Resources==
==Genetic Resources==
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|- {{table_heading_style}}
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
|-
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JW2786
JW2786
Line 153: Line 570:
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
Line 160: Line 577:


Primer 2:CCgCCTAAATCCGCCATCAACAC  
Primer 2:CCgCCTAAATCCGCCATCAACAC  
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[[:Category:Kohara_Phage:10B6|10B6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=459 Genobase]
|
PMID:3038334
|-
|
[[fucP:Gene|fucP3072::Tn10]]
|
Linked marker
|
CAG12079 = [http://cgsc2.biology.yale.edu/Strain.php?ID=38034 CGSC7427]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 59% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:fuc-3072::Tn10
|-
|
[[recD:Gene|recD1901::Tn10]]
|
Linked marker
|
CAG12135 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40106 CGSC7429]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 89% <ref name='LIB:Nichols_linked_Tn'/>
 
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===Notes===
===Notes===
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10063 EcoCyc:EG10063] 
|
|
[[EcoCyc]]
|-
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
EcoCyc:EG10063
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0061 EchoBASE:EB0061]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[EcoGene]]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
EcoGene:EG10063
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2818 EcoliGenExpDB:b2818]
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
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[[RegulonDB]]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C3412 ECOL199310CYC:C3412] 
RegulonDB:ECK120000059
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10063 EcoGene:EG10063]
[http://www.ncbi.nlm.nih.gov/gene?term=947289 GeneID:947289]
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Escherichia coli str. K-12 substr. MG1655
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
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[http://BioCyc.org/ECOO157/new-image?object=ARGA ECOO157CYC:ARGA] 
EchoBASE:EB0061
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/1021 CGSC:1021] 
ASAP:ABE-0009245
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:arg genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:44, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

argA

Mnemonic

Arginine

Synonyms

ECK2814, b2818, JW2786, Arg1, Arg2[1], Arg2, argB


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.52 minutes 

MG1655: 2947264..2948595
<gbrowseImage> name=NC_000913:2947264..2948595 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2843861..2845192
<gbrowseImage> name=NC_012967:2843861..2845192 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2834412..2835743
<gbrowseImage> name=NC_012759:2834412..2835743 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2947898..2949229
<gbrowseImage> name=NC_007779:2947898..2949229 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3041134..3042465
<gbrowseImage> name=NC_010473:3041134..3042465 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2947267

Edman degradation

PMID:3320971[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

argAR58H

R58H

Auxotrophies

In EE11

seeded from UniProt:P0A6C5

argAH15Y

H15Y

Auxotrophies

In EE17

seeded from UniProt:P0A6C5

argAY19C

Y19C

Auxotrophies

In EE51

seeded from UniProt:P0A6C5

argAG287S

G287S

Auxotrophies

In PT2M216

seeded from UniProt:P0A6C5

argAQ432R

Q432R

Auxotrophies

In PT2M217

seeded from UniProt:P0A6C5

argAS54N

S54N

Auxotrophies

In PT2M217

seeded from UniProt:P0A6C5

ΔargA (Keio:JW2786)

deletion

deletion

Auxotrophies

PMID:16738554[3]

Shigen

CGSC10181[4]

argA::Tn5KAN-I-SceI (FB20943)

Insertion at nt 933 in Plus orientation

Auxotrophies

PMID:15262929[5]

E. coli Genome Project:FB20943

contains pKD46

argA::Tn5KAN-I-SceI (FB20944)

Insertion at nt 933 in Plus orientation

Auxotrophies

PMID:15262929[5]

E. coli Genome Project:FB20944

does not contain pKD46

argA21

Auxotrophies

CGSC:5137

argA42

Auxotrophies

CGSC:5157

argA52

Auxotrophies

CGSC:5167

argA76(Fs)

Auxotrophies

CGSC:5192

frameshift mutation

argA103

Auxotrophies

CGSC:5207

argA22

Auxotrophies

CGSC:6000

argA81::Tn10

Auxotrophies

CGSC:7098

argA9999

Auxotrophies

CGSC:9194

ΔargA743::kan

deletion

deletion

Auxotrophies

PMID:16738554[3]

CGSC:100036



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2786

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGTAAAGGAACGTAAAACCGA

Primer 2:CCgCCTAAATCCGCCATCAACAC

10B6

Kohara Phage

Genobase

PMID:3038334[7]

fucP3072::Tn10

Linked marker

CAG12079 = CGSC7427[4]

est. P1 cotransduction: 59% [8]
Synonyms:fuc-3072::Tn10

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[4]

est. P1 cotransduction: 89% [8]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10063

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10063

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000059

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947289

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0061

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009245

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Brown, K et al. (1987) Complete nucleotide sequence of the Escherichia coli argA gene. Nucleic Acids Res. 15 10586 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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