argA:Gene: Difference between revisions
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|{{Sub}}[[#Sequence Features|Sequence Features]] | |{{Sub}}[[#Sequence Features|Sequence Features]] | ||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
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!align=left {{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
|| | || | ||
''ECK2814, b2818, JW2786, Arg1, Arg2''<ref name='LIB:Riley_2006'/> | ''ECK2814, b2818, JW2786, Arg1, Arg2''<ref name='LIB:Riley_2006'/>, Arg2, argB'' | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
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* | * | ||
****************************************************************************************** -->{{Gene_location_help}} | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9e6ff48a2a" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
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MG1655: 2947264..2948595<br /><gbrowseImage> | MG1655: 2947264..2948595<br /><gbrowseImage> | ||
name=NC_000913:2947264..2948595 | name=NC_000913:2947264..2948595 | ||
source= | source=MG1655 | ||
preset=GeneLocation | preset=GeneLocation | ||
</gbrowseImage> | </gbrowseImage> | ||
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*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10063 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10063 EcoCyc] | ||
{{GBrowseLink| | {{GBrowseLink|MG1655|NC_000913|2946264|2949595|GBrowse(MG1655)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|MG1655|NC_000913|2947264|2948595|FASTA Dump}} | ||
*[http://biocyc.org/ECOLI/sequence?object=EG10063 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=EG10063 EcoCyc Sequence Dump] | ||
|- | |- | ||
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{{GBrowseLink|BW2952| | {{GBrowseLink|BW2952|NC_012759|2834412|2835743|GBrowse (BW2952)}} | ||
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{{GBrowseFastaLink|BW2952| | {{GBrowseFastaLink|BW2952|NC_012759|2834412|2835743|FASTA Dump}} | ||
|- | |- | ||
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W3110: 2947898..2949229<br /><gbrowseImage> | W3110: 2947898..2949229<br /><gbrowseImage> | ||
name= | name=NC_007779:2947898..2949229 | ||
source= | source=W3110 | ||
preset=GeneLocation | preset=GeneLocation | ||
</gbrowseImage> | </gbrowseImage> | ||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|W3110|NC_007779|2946898|2950229|GBrowse(W3110)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|W3110|NC_007779|2947898|2949229|FASTA Dump}} | ||
|- | |- | ||
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DH10B: 3041134..3042465<br /><gbrowseImage> | DH10B: 3041134..3042465<br /><gbrowseImage> | ||
name=NC_010473:3041134..3042465 | name=NC_010473:3041134..3042465 | ||
source= | source=DH10B | ||
preset=GeneLocation | preset=GeneLocation | ||
</gbrowseImage> | </gbrowseImage> | ||
| | | | ||
{{GBrowseLink| | {{GBrowseLink|DH10B|NC_010473|3038472|3045127|GBrowse(DH10B)}} | ||
| | | | ||
{{GBrowseFastaLink| | {{GBrowseFastaLink|DH10B|NC_010473|3038472|3045127|FASTA Dump}} | ||
|- class="tableEdit_footer" | |- class="tableEdit_footer" | ||
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* | * | ||
****************************************************************************************** -->{{Gene_sequence_features_help}} | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="X46a9e70004884" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
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* | * | ||
****************************************************************************************** -->{{Gene_alleles_help}} | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9e7004b9db" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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R58H | R58H | ||
| | | | ||
Auxotrophies | |||
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In EE11 | In EE11 | ||
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H15Y | H15Y | ||
| | | | ||
Auxotrophies | |||
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In EE17 | In EE17 | ||
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Y19C | Y19C | ||
| | | | ||
Auxotrophies | |||
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In EE51 | In EE51 | ||
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G287S | G287S | ||
| | | | ||
Auxotrophies | |||
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In PT2M216 | In PT2M216 | ||
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Q432R | Q432R | ||
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Auxotrophies | |||
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In PT2M217 | In PT2M217 | ||
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S54N | S54N | ||
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Auxotrophies | |||
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In PT2M217 | In PT2M217 | ||
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|- | |- | ||
| | | | ||
ΔargA (Keio:JW2786) | |||
| | | | ||
deletion | deletion | ||
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deletion | deletion | ||
| | | | ||
Auxotrophies | |||
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PMID:16738554 | PMID:16738554 | ||
| | | | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen] | ||
[http://cgsc2.biology.yale.edu/Strain.php?ID=108462 CGSC10181]<ref name='LIB:cgsc'/> | |||
| | | | ||
|- | |- | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN-I-SceI] (FB20943) | ||
| | | | ||
Insertion at nt 933 in Plus orientation | |||
| | | | ||
| | | | ||
Auxotrophies | |||
| | | | ||
| | | | ||
PMID: | PMID:15262929 | ||
| | | | ||
[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20943] | ||
| | | | ||
contains [[pKD46]] | |||
|- | |- | ||
| | | | ||
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0009245 argA::Tn5KAN-I-SceI] (FB20944) | ||
| | | | ||
Insertion at nt 933 in Plus orientation | |||
| | | | ||
| | | | ||
Auxotrophies | |||
| | | | ||
| | | | ||
PMID: | PMID:15262929 | ||
| | | | ||
[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20944] | ||
| | | | ||
does not contain [[pKD46]] | |||
|- | |- | ||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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Auxotrophies | |||
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|- | |- | ||
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'' | ''ΔargA743::kan'' | ||
| | | | ||
deletion | |||
| | | | ||
deletion | |||
| | | | ||
Auxotrophies | |||
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===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.16203.A4e04b77dd16f0--> | |||
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* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
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****************************************************************************************** -->{{Genetic_interaction_help}} | |||
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="A4e04b77dd16f0" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.16203.A4e04b77dd16f0&page=16203&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
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<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.16203.A4e04b77dd16f0--></protect> | |||
<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
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* | * | ||
****************************************************************************************** -->{{Gene_resources_help}} | ****************************************************************************************** -->{{Gene_resources_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="A46a9e70107243" class=" tableEdit Gene_resource_table" | ||
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!|Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
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Primer 2:CCgCCTAAATCCGCCATCAACAC | Primer 2:CCgCCTAAATCCGCCATCAACAC | ||
|- | |||
| | |||
[[:Category:Kohara_Phage:10B6|10B6]] | |||
| | |||
Kohara Phage | |||
| | |||
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=459 Genobase] | |||
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PMID:3038334 | |||
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****************************************************************************************** -->{{Gene_accessions_help}} | ****************************************************************************************** -->{{Gene_accessions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M46a9e7019f8e9" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
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[ | [[EcoCyc]] | ||
[ | |||
| | | | ||
EcoCyc:EG10063 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[EcoGene]] | |||
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EcoGene:EG10063 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[RegulonDB]] | |||
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RegulonDB:ECK120000059 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
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[http:// | [http://www.ncbi.nlm.nih.gov/gene?term=947289 GeneID:947289] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
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[[EchoBASE]] | |||
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EchoBASE:EB0061 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
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[[ASAP]] | |||
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ASAP:ABE-0009245 | |||
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
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{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M46a7034466271" class=" tableEdit Links_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!|Name!!URL!!Comments | !|Name!!URL!!Comments | ||
Latest revision as of 20:44, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
argA |
|---|---|
| Mnemonic |
Arginine |
| Synonyms |
ECK2814, b2818, JW2786, Arg1, Arg2[1], Arg2, argB |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
63.52 minutes |
MG1655: 2947264..2948595 |
||
|
NC_012967: 2843861..2845192 |
||||
|
NC_012759: 2834412..2835743 |
||||
|
W3110 |
|
W3110: 2947898..2949229 |
||
|
DH10B: 3041134..3042465 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
2947267 |
Edman degradation |
| ||
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
argAR58H |
R58H |
Auxotrophies |
In EE11 |
seeded from UniProt:P0A6C5 | |||
|
argAH15Y |
H15Y |
Auxotrophies |
In EE17 |
seeded from UniProt:P0A6C5 | |||
|
argAY19C |
Y19C |
Auxotrophies |
In EE51 |
seeded from UniProt:P0A6C5 | |||
|
argAG287S |
G287S |
Auxotrophies |
In PT2M216 |
seeded from UniProt:P0A6C5 | |||
|
argAQ432R |
Q432R |
Auxotrophies |
In PT2M217 |
seeded from UniProt:P0A6C5 | |||
|
argAS54N |
S54N |
Auxotrophies |
In PT2M217 |
seeded from UniProt:P0A6C5 | |||
|
ΔargA (Keio:JW2786) |
deletion |
deletion |
Auxotrophies |
||||
|
argA::Tn5KAN-I-SceI (FB20943) |
Insertion at nt 933 in Plus orientation |
Auxotrophies |
contains pKD46 | ||||
|
argA::Tn5KAN-I-SceI (FB20944) |
Insertion at nt 933 in Plus orientation |
Auxotrophies |
does not contain pKD46 | ||||
|
argA21 |
Auxotrophies |
||||||
|
argA42 |
Auxotrophies |
||||||
|
argA52 |
Auxotrophies |
||||||
|
argA76(Fs) |
Auxotrophies |
frameshift mutation | |||||
|
argA103 |
Auxotrophies |
||||||
|
argA22 |
Auxotrophies |
||||||
|
argA81::Tn10 |
Auxotrophies |
||||||
|
argA9999 |
Auxotrophies |
||||||
|
ΔargA743::kan |
deletion |
deletion |
Auxotrophies |
| |||
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2786 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGTAAAGGAACGTAAAACCGA Primer 2:CCgCCTAAATCCGCCATCAACAC | |
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 59% [8] | ||
|
Linked marker |
est. P1 cotransduction: 89% [8] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Brown, K et al. (1987) Complete nucleotide sequence of the Escherichia coli argA gene. Nucleic Acids Res. 15 10586 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ 5.0 5.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).