yffL:Gene: Difference between revisions
keio clone record corrected by bot |
touch |
||
| (31 intermediate revisions by 2 users not shown) | |||
| Line 14: | Line 14: | ||
|{{Sub}}[[#Nomenclature |Nomenclature]] | |{{Sub}}[[#Nomenclature |Nomenclature]] | ||
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | |{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]] | ||
|{{Sub}}[[#Sequence Features|Sequence Features]] | |||
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | |{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]] | ||
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]] | |||
|{{Sub}}[[#Genetic Resources |Genetic Resources]] | |{{Sub}}[[#Genetic Resources |Genetic Resources]] | ||
|{{Sub}}[[#Accessions in Other Databases |Accessions]] | |{{Sub}}[[#Accessions in Other Databases |Accessions]] | ||
| Line 27: | Line 29: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_nomenclature_help}} | ||
{| id="A46a9de27a3c13" class=" tableEdit Gene_nomenclature_table" | |||
|- | |- | ||
!{{table_heading_style}} |Standard name | !align=left {{table_heading_style}} |Standard name | ||
| | || | ||
''yffL'' | ''yffL'' | ||
|- | |- | ||
!{{table_heading_style}} |Mnemonic | !align=left {{table_heading_style}} |Mnemonic | ||
| | || | ||
Systematic nomenclature | |||
|- | |- | ||
!{{table_heading_style}} |Synonyms | !align=left {{table_heading_style}} |Synonyms | ||
| | || | ||
''ECK2438, b2443, unique to MG1655''<ref name='LIB:Riley_2006'/> | ''ECK2438, b2443, unique to MG1655''<ref name='LIB:Riley_2006'/> | ||
|- | |||
| | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.A46a9de27a3c13&page=15451&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.A46a9de27a3c13--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.A46a9de27a3c13--> | ||
<!--Nomenclature_notes--></protect> | <!--Nomenclature_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
| Line 59: | Line 63: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_location_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="A46a9de282d692" class=" tableEdit Gene_location_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | !|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links | ||
|- | |- | ||
| | | | ||
MG1655 | MG1655 | ||
| Line 72: | Line 76: | ||
55.14 minutes | 55.14 minutes | ||
| | | | ||
MG1655: 2558279..2558920<br /> | MG1655: 2558279..2558920<br /><gbrowseImage> | ||
name=NC_000913:2558279..2558920 | |||
source=MG1655 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | | | ||
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G7273 EcoCyc] | *[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=G7273 EcoCyc] | ||
{{GBrowseLink|MG1655|NC_000913|2557279|2559920|GBrowse(MG1655)}} | |||
| | | | ||
{{GBrowseFastaLink|MG1655|NC_000913|2558279|2558920|FASTA Dump}} | |||
*[http://biocyc.org/ECOLI/sequence?object=G7273 EcoCyc Sequence Dump] | *[http://biocyc.org/ECOLI/sequence?object=G7273 EcoCyc Sequence Dump] | ||
|-class= | |- | ||
|[{{SERVER}} | | | ||
[[BW2952]] | |||
| | |||
| | |||
NC_012759: 2444084..2444725<br /><gbrowseImage> | |||
name=NC_012759:2444084..2444725 | |||
source=BW2952 | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|BW2952|NC_012759|2444084|2444725|GBrowse (BW2952)}} | |||
| | |||
{{GBrowseFastaLink|BW2952|NC_012759|2444084|2444725|FASTA Dump}} | |||
|- | |||
| | |||
[[DH10B]] | |||
| | |||
| | |||
DH10B: 2650044..2650685<br /><gbrowseImage> | |||
name=NC_010473:2650044..2650685 | |||
source=DH10B | |||
preset=GeneLocation | |||
</gbrowseImage> | |||
| | |||
{{GBrowseLink|DH10B|NC_010473|2648762|2651967|GBrowse(DH10B)}} | |||
| | |||
{{GBrowseFastaLink|DH10B|NC_010473|2648762|2651967|FASTA Dump}} | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.A46a9de282d692&page=15451&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_location_table edit table]</span> || || || || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.A46a9de282d692--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.A46a9de282d692--> | ||
<!--Location_notes--></protect> | <!--Location_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
| Line 94: | Line 136: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_sequence_features_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="F46a9de287d3b9" class=" tableEdit Gene_sequence_features_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Feature | !|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes | ||
|-class= | |||
|[{{SERVER}} | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.F46a9de287d3b9&page=15451&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_sequence_features_table edit table]</span> || || || || || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.F46a9de287d3b9--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.F46a9de287d3b9--> | ||
<protect><!--Sequence_notes--></protect> | <protect><!--Sequence_notes--></protect> | ||
====Notes==== | ====Notes==== | ||
| Line 115: | Line 158: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_alleles_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="K46a9de28c2e09" class=" tableEdit Gene_allele_table dataTable" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
| | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.K46a9de28c2e09&page=15451&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || || | |||
| | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.K46a9de28c2e09--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.K46a9de28c2e09--> | ||
<protect><!--Alleles_notes--></protect> | <protect><!--Alleles_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
==Genetic Interactions== | |||
{{BacktoTop}} | |||
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.15451.M4e04c928244b5--> | |||
<!-- | |||
****************************************************************************************** | |||
* | |||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | |||
* | |||
****************************************************************************************** -->{{Genetic_interaction_help}} | |||
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M4e04c928244b5" class=" tableEdit Genetic_interaction_table dataTable" | |||
|- {{table_heading_style}} | |||
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes | |||
|- class="tableEdit_footer" | |||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.15451.M4e04c928244b5&page=15451&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || || | |||
|} | |||
<!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.15451.M4e04c928244b5--></protect> | |||
<!-- Genetic Interacdtions Notes --> | |||
===Notes=== | |||
==Genetic Resources== | ==Genetic Resources== | ||
| Line 153: | Line 202: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_resources_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Z46a9de297b6d7" class=" tableEdit Gene_resource_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Resource!!Resource Type!!Source!!Notes/Reference | !|Resource!!Resource Type!!Source!!Notes/Reference | ||
|- | |- | ||
| | | | ||
| Line 168: | Line 217: | ||
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | [http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen] | ||
| | | | ||
PMID:16769691 | |||
Status: | Status: | ||
| Line 175: | Line 224: | ||
Primer 2: | Primer 2: | ||
|- | |- | ||
| | | | ||
[[nupC:Gene|nupC510::Tn10]] | [[nupC:Gene|nupC510::Tn10]] | ||
| Line 184: | Line 233: | ||
| | | | ||
est. P1 cotransduction: 11% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 11% <ref name='LIB:Nichols_linked_Tn'/> | ||
|- | |- | ||
| | | | ||
[[purC:Gene|purC80::Tn10]] | [[purC:Gene|purC80::Tn10]] | ||
| Line 193: | Line 242: | ||
| | | | ||
est. P1 cotransduction: 24% <ref name='LIB:Nichols_linked_Tn'/> | est. P1 cotransduction: 24% <ref name='LIB:Nichols_linked_Tn'/> | ||
|-class= | |||
|[{{SERVER}} | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.Z46a9de297b6d7&page=15451&pagename={{FULLPAGENAMEE}}&type=&template=Gene_resource_table edit table]</span> || || || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.Z46a9de297b6d7--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.Z46a9de297b6d7--> | ||
<protect><!--Gene_resources_notes--></protect> | <protect><!--Gene_resources_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
| Line 207: | Line 257: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** -->{{Gene_accessions_help}} | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="I46a9de2a1c972" class=" tableEdit Gene_accessions_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Database!!Accession!!Notes | !|Database!!Accession!!Notes | ||
|- | |- | ||
| | |||
[[EcoCyc]] | |||
| | |||
EcoCyc:G7273 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | |||
[[EcoGene]] | |||
| | |||
EcoGene:EG14173 | |||
| | |||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |||
| | | | ||
[[RegulonDB]] | |||
| | | | ||
RegulonDB:ECK120003847 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[http://www. | [http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]]) | ||
| | | | ||
[http://www. | [http://www.ncbi.nlm.nih.gov/gene?term=946922 GeneID:946922] | ||
| | | | ||
Escherichia coli str. K-12 substr. MG1655 | |||
|- | |- | ||
| | | | ||
[[EchoBASE]] | |||
| | | | ||
EchoBASE:EB3925 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|- | |- | ||
| | | | ||
[[ASAP]] | |||
| | | | ||
ASAP:ABE-0008055 | |||
| | | | ||
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]] | |||
|-class= | |||
|[{{SERVER}} | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.I46a9de2a1c972&page=15451&pagename={{FULLPAGENAMEE}}&type=0&template=Gene_accessions_table edit table]</span> || || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.I46a9de2a1c972--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.I46a9de2a1c972--> | ||
<protect><!--Gene_accessions_notes--></protect> | <protect><!--Gene_accessions_notes--></protect> | ||
===Notes=== | ===Notes=== | ||
| Line 256: | Line 321: | ||
<!-- | <!-- | ||
****************************************************************************************** | ****************************************************************************************** | ||
* | * | ||
* ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | * ** PLEASE DON'T EDIT THIS TABLE DIRECTLY. Use the edit table link under the table. ** | ||
* | * | ||
****************************************************************************************** --> | ****************************************************************************************** --> | ||
{| | {| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="M46a6fb17d53c7" class=" tableEdit Links_table" | ||
|- {{table_heading_style}} | |- {{table_heading_style}} | ||
!Name!!URL!!Comments | !|Name!!URL!!Comments | ||
|-class= | |||
|[{{SERVER}} | |- class="tableEdit_footer" | ||
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.15451.M46a6fb17d53c7&page=15451&pagename={{FULLPAGENAMEE}}&type=0&template=Links_table edit table]</span> || || | |||
|} | |} | ||
<!--box uid=421aa90e079fa326b6494f812ad13e79.15451.M46a6fb17d53c7--> | <!--box uid=421aa90e079fa326b6494f812ad13e79.15451.M46a6fb17d53c7--> | ||
<noinclude><protect> | <noinclude><protect> | ||
| Line 273: | Line 339: | ||
{{BacktoTop}} | {{BacktoTop}} | ||
{{RefHelp}} | {{RefHelp}} | ||
<references/> | <references /> | ||
__NOTOC__ | __NOTOC__ | ||
===Categories=== | ===Categories=== | ||
[[Category:Gene List:MG1655]] | [[Category:Gene List:MG1655]] | ||
[[Category:Genes not in the Keio knockout collection]] | [[Category:Genes not in the Keio knockout collection]] | ||
[[Category:Genes in the ASKA clone collection]] | [[Category:Genes in the ASKA clone collection]] | ||
[[Category:Gene List:Eut/CPZ-55 prophage]] | |||
[[Category:yff genes]][[Category:Gene_List:DH10B]] | |||
[[Category:y genes]] | |||
[[Category:y genes]] | |||
[[Category:Gene_List:BW2952]] | |||
Latest revision as of 20:42, 19 September 2013
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
yffL |
|---|---|
| Mnemonic |
Systematic nomenclature |
| Synonyms |
ECK2438, b2443, unique to MG1655[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
55.14 minutes |
MG1655: 2558279..2558920 |
||
|
NC_012759: 2444084..2444725 |
||||
|
DH10B: 2650044..2650685 |
||||
| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Interactions
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
Plasmid clone |
Status: Primer 1: Primer 2: | ||
|
Linked marker |
est. P1 cotransduction: 11% [4] | ||
|
Linked marker |
est. P1 cotransduction: 24% [4] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).