yfiD:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

yfiD

Mnemonic

Systematic nomenclature

Synonyms

ECK2577, b2579, JW2563[1], grcA, JW2563

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

58.5 minutes, 58.5 minutes 

MG1655: 2714471..2714088
<gbrowseImage> name=NC_000913:2714088..2714471 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2637942..2637559
<gbrowseImage> name=NC_012967:2637559..2637942 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2599893..2600276
<gbrowseImage> name=NC_012759:2599893..2600276 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2715105..2714722
<gbrowseImage> name=NC_007779:2714722..2715105 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2806236..2805853
<gbrowseImage> name=NC_010473:2805853..2806236 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2714088

Edman degradation

PMID:9179852[2]
PMID:9600841[3]
PMID:9740056[4]
PMID:9868784[5]
PMID:10094700[6]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔyfiD (Keio:JW2563)

deletion

deletion

PMID:16738554[7]

Shigen
CGSC10038[8]

ΔyfiD747::kan

PMID:16738554[7]

CGSC:104798


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2563

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCATTACAGGTATCCAGATTAC

Primer 2:CCaAGGCTTTCAGTAAAGGTACG

4A12

Kohara Phage

Genobase

PMID:3038334[10]

3F10

Kohara Phage

Genobase

PMID:3038334[10]

nadB51::Tn10

Linked marker

CAG18480 = CGSC7419[8]

est. P1 cotransduction: 86% [11]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[8]

est. P1 cotransduction: 42% [11]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (EcoliWiki Page)

GeneID:947068

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Green, J & Baldwin, ML (1997) HlyX, the FNR homologue of Actinobacillus pleuropneumoniae, is a [4Fe-4S]-containing oxygen-responsive transcription regulator that anaerobically activates FNR-dependent class I promoters via an enhanced AR1 contact. Mol. Microbiol. 24 593-605 PubMed
  3. Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  4. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  5. Wasinger, VC & Humphery-Smith, I (1998) Small genes/gene-products in Escherichia coli K-12. FEMS Microbiol. Lett. 169 375-82 PubMed
  6. Blankenhorn, D et al. (1999) Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis. J. Bacteriol. 181 2209-16 PubMed
  7. 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  8. 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
  9. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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