wbbL 1:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

wbbL_1

Mnemonic
Synonyms

wbbL, ECK2025, b2031, JW2016, yefJ, b4540, JW2012, b4571, JW2016+JW2012, wbbL_2[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

45.28 minutes 

MG1655: 2101413..2100940
<gbrowseImage> name=NC_000913:2100940..2101413 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2105526..2105053
<gbrowseImage> name=NC_007779:2105053..2105526 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

45.25 minutes 

MG1655: 2099701..2099420
<gbrowseImage> name=NC_000913:2099420..2099701 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2103814..2103533
<gbrowseImage> name=NC_007779:2103533..2103814 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

 

MG1655: 2101413..2099420
<gbrowseImage> name=NC_000913:2099420..2101413 source=ecoli preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2105526..2103533
<gbrowseImage> name=NC_007779:2103533..2105526 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yefJ::Tn5KAN-I-SceI (FB20584)

Insertion at nt 116 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20584

contains pKD46

yefJ::Tn5KAN-I-SceI (FB20585)

Insertion at nt 116 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20585

does not contain pKD46

ΔwbbL728::kan

PMID:16738554[5]

CGSC:103366


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[6]

Status:

Primer 1:

Primer 2:

21H10

Kohara Phage

Genobase

PMID:3038334[7]

1G1

Kohara Phage

Genobase

PMID:3038334[7]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[8]

est. P1 cotransduction: 53% [9]
Synonyms:zee-3129::Tn10

zbd-3104::Tn10

Linked marker

CAG12021 = CGSC7344[8]

est. P1 cotransduction: 1% [9]
Synonyms:zbc-3105::Tn10, zbd-3105::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:4056030

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001911

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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