usg:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

usg

Mnemonic

Upstream gene

Synonyms

ECK2313, b2319, JW2316, usg-1[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

52.45 minutes 

MG1655: 2434671..2433658
<gbrowseImage> name=NC_000913:2433658..2434671 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2380241..2379228
<gbrowseImage> name=NC_012967:2379228..2380241 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2319463..2320476
<gbrowseImage> name=NC_012759:2319463..2320476 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2442095..2441082
<gbrowseImage> name=NC_007779:2441082..2442095 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2526436..2525423
<gbrowseImage> name=NC_010473:2525423..2526436 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2433658

Edman degradation

PMID:2681152[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δusg (Keio:JW2316)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9858[5]

Δusg-728::kan

PMID:16738554[4]

CGSC:103330


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2316

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCTGAAGGCTGGAACATTGC

Primer 2:CCGTACAGATACTCCTGCACCAG

12F12

Kohara Phage

Genobase

PMID:3038334[7]

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[5]

est. P1 cotransduction: 14% [8]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[5]

est. P1 cotransduction: 5% [8]
Synonyms:zfb-1::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11059

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11059

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001048

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946797

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1052

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0007660

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Schoenlein, PV et al. (1989) Divergent transcription of pdxB and homology between the pdxB and serA gene products in Escherichia coli K-12. J. Bacteriol. 171 6084-92 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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