trpA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TrpA

Synonyms

tryptophan synthase, alpha subunit[1], B1260[2][1], Try[2][1], TrpA[2][1], α subunit[2][1], TSase α[2][1] , ECK1254, JW1252, try, tryp, b1260

Product description

tryptophan synthase, α subunit[2][3];

Component of tryptophan synthase[2][3]

Tryptophan synthase, alpha subunit[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000162

tryptophan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00131

P

Seeded from EcoCyc (v14.0)

complete

GO:0000162

tryptophan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0822

P

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011060

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013785

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00131

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002028

F

Seeded from EcoCyc (v14.0)

complete

GO:0004834

tryptophan synthase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.2.1.20

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:378665[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0016829

lyase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0456

F

Seeded from EcoCyc (v14.0)

complete

GO:0043199

sulfate binding

PMID:15451433[6]

IPI: Inferred from Physical Interaction

F

Missing: with/from

GO:0005515

protein binding

PMID:378665[5]

IDA: Inferred from Direct Assay

F

Tryptophan synthase is a heterotetramer (2 alpha subunits & 2 beta subunits) EcoliWiki:trpA|EcoliWiki:trpB


complete

Contributes to

GO:0004834

tryptophan synthase activity

PMID:8463331[7]

IDA: Inferred from Direct Assay

F

complete

GO:0009073

aromatic amino acid family biosynthetic process

PMID:8463331[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000162

tryptophan biosynthetic process

PMID:8463331[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005737

cytoplasm

PMID:4887511[8]

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of tryptophan synthase

could be indirect

Protein

yciE

PMID:16606699[9]

Experiment(s):EBI-1139344

Protein

yhhY

PMID:16606699[9]

Experiment(s):EBI-1139344

Protein

nadE

PMID:16606699[9]

Experiment(s):EBI-1139344
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MERYESLFAQ LKERKEGAFV PFVTLGDPGI EQSLKIIDTL IEAGADALEL GIPFSDPLAD
GPTIQNATLR AFAAGVTPAQ CFEMLALIRQ KHPTIPIGLL MYANLVFNKG IDEFYAQCEK
VGVDSVLVAD VPVEESAPFR QAALRHNVAP IFICPPNADD DLLRQIASYG RGYTYLLSRA
GVTGAENRAA LPLNHLVAKL KEYNAAPPLQ GFGISAPDQV KAAIDAGAAG AISGSAIVKI
IEQHINEPEK MLAALKVFVQ PMKAATRS
Length

268

Mol. Wt

28.724 kDa

pI

5.3 (calculated)

Extinction coefficient

10,430 - 10,805 (calc based on 7 Y, 0 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

8..267

PF00290 Tryptophan synthase alpha chain

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=trpA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129221

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946204

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004232

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A877

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11024

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11024

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946204

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001013

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1017

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Gschwind, HP et al. (1979) Affinity chromatography of tryptophan synthase from Escherichia coli. Systematic studies with immobilized tryptophanol phosphate. Eur. J. Biochem. 96 403-16 PubMed
  6. ↑ Jeong, MS et al. (2004) Structures of wild-type and P28L/Y173F tryptophan synthase alpha-subunits from Escherichia coli. Biochem. Biophys. Res. Commun. 323 1257-64 PubMed
  7. ↑ 7.0 7.1 7.2 Yanofsky, C et al. (1993) Partial revertants of tryptophan synthetase alpha chain active site mutant Asp60-->Asn. J. Biol. Chem. 268 8213-20 PubMed
  8. ↑ Murphy, TM & Mills, SE (1969) Immunochemical and enzymatic comparisons of the tryptophan synthase alpha subunits from five species of Enterobacteriaceae. J. Bacteriol. 97 1310-20 PubMed
  9. ↑ 9.0 9.1 9.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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