PMID:21321206
| Citation |
Cho, H, McManus, HR, Dove, SL and Bernhardt, TG (2011) Nucleoid occlusion factor SlmA is a DNA-activated FtsZ polymerization antagonist. Proc. Natl. Acad. Sci. U.S.A. 108:3773-8 |
|---|---|
| Abstract |
The tubulin-like FtsZ protein initiates assembly of the bacterial cytokinetic machinery by polymerizing into a ring structure, the Z ring, at the prospective site of division. To block Z-ring formation over the nucleoid and help coordinate cell division with chromosome segregation, Escherichia coli employs the nucleoid-associated division inhibitor, SlmA. Here, we investigate the mechanism by which SlmA regulates FtsZ assembly. We show that SlmA disassembles FtsZ polymers in vitro. In addition, using chromatin immunoprecipitation (ChIP), we identified 24 SlmA-binding sequences (SBSs) on the chromosome. Remarkably, SlmA binding to SBSs dramatically enhanced its ability to interfere with FtsZ polymerization, and ChIP studies indicate that SlmA regulates FtsZ assembly at these sites in vivo. Because of the dynamic and highly organized nature of the chromosome, coupling SlmA activation to specific DNA binding provides a mechanism for the precise spatiotemporal control of its anti-FtsZ activity within the cell. |
| Links |
PubMed PMC3048121 Online version:10.1073/pnas.1018674108 |
| Keywords |
Bacterial Proteins/antagonists & inhibitors; Bacterial Proteins/metabolism; Bacterial Proteins/ultrastructure; Base Sequence; Binding Sites; Carrier Proteins/metabolism; Chromosomes, Bacterial/metabolism; Cytoskeletal Proteins/antagonists & inhibitors; Cytoskeletal Proteins/metabolism; Cytoskeletal Proteins/ultrastructure; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Models, Biological; Molecular Sequence Data; Polymerization; Protein Binding |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<protect><annotationlinks/></protect>
Data
<protect>
| Strain/Organism | Assay | Analyte | Variable Conditions | Notes | Links |
|---|---|---|---|---|---|
|
MG1655 |
ChIP-chip |
SlmA |
|||
| edit table |
</protect>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.
