secB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

secB

Mnemonic

secretion Secretory

Synonyms

ECK3599, b3609, JW3584[1], JW3584

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

81.51 minutes 

MG1655: 3782151..3781684
<gbrowseImage> name=NC_000913:3781684..3782151 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3724894..3724427
<gbrowseImage> name=NC_012967:3724427..3724894 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3670010..3670477
<gbrowseImage> name=NC_012759:3670010..3670477 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3856287..3856754
<gbrowseImage> name=NC_007779:3856287..3856754 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3879728..3879261
<gbrowseImage> name=NC_010473:3879261..3879728 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3781687

Edman degradation

PMID:2644258[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔsecB (Keio:JW3584)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10640[4]

secB8

PMID:6403503[5]

null

ΔsecB721::kan

PMID:16738554[3]

CGSC:102902


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3584

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCAGAACAAAACAACACTGA

Primer 2:CCGGCATCCTGATGTTCTTCAGT

5D2

Kohara Phage

Genobase

PMID:3038334[7]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: % [8]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: 13% [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10937

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10937

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000926

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948123

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0930

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011798

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kumamoto, CA et al. (1989) Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system. J. Biol. Chem. 264 2242-9 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kumamoto, CA & Beckwith, J (1983) Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J. Bacteriol. 154 253-60 PubMed
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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