secB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

secB

Mnemonic

secretion Secretory

Synonyms

ECK3599, b3609, JW3584[1], JW3584


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

81.51 minutes 

MG1655: 3782151..3781684
<gbrowseImage> name=NC_000913:3781684..3782151 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3724894..3724427
<gbrowseImage> name=NC_012967:3724427..3724894 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3670010..3670477
<gbrowseImage> name=NC_012759:3670010..3670477 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3856287..3856754
<gbrowseImage> name=NC_007779:3856287..3856754 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3879728..3879261
<gbrowseImage> name=NC_010473:3879261..3879728 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3781687

Edman degradation

PMID:2644258[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔsecB (Keio:JW3584)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10640[4]

secB8

PMID:6403503[5]

null

ΔsecB721::kan

PMID:16738554[3]

CGSC:102902



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3584

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCAGAACAAAACAACACTGA

Primer 2:CCGGCATCCTGATGTTCTTCAGT

5D2

Kohara Phage

Genobase

PMID:3038334[7]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: % [8]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: 13% [8]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10937

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10937

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000926

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948123

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0930

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011798

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kumamoto, CA et al. (1989) Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system. J. Biol. Chem. 264 2242-9 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kumamoto, CA & Beckwith, J (1983) Mutations in a new gene, secB, cause defective protein localization in Escherichia coli. J. Bacteriol. 154 253-60 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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