rseP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

rseP

Gene Synonym(s)

yaeL, ECK0175, b0176, JW0171, ecf, ecfE[1][2][3], ecfE

Product Desc.

zinc metallopeptidase[3]; RseP zinc protease[2]

Inner membrane zinc RIP metalloprotease; activates RpoE by degrading RseA; multicopy rpoE suppresses rseP mutation[4]

Product Synonyms(s)

zinc metallopeptidase[1], B0176[3][1], EcfE[3][1], YaeL[3][1], RseP[3][1] , ecfE, ECK0175, JW0171, yaeL, b0176

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): yaeL[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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RpoE regulon. RseP activates RpoE and releases membrane-bound RseA-RpoE complexes. Cleavage occurs around RseA membrane domain generating the N-terminal and the soluable product. rseP is an essential gene, but deletion of ompCF or ompCA (or an sRNA rseX clone, which suppresses ompCA translation) allows growth. RseP or some other protease degrades RseA in the released complexes. The RseA periplasmic domain must be first clipped or degraded by DegS before it is a substrate for RseP. RseP is related to the E. faecalis Eep protein involved in bacterial regulated intramembrane proteolysis (RIP). RseP has been topology mapped using protein fusions. RseP is reported to degrade RpoE and RpoH in vitro and to be an negative, not positive, effector of RpoE action in vivo, but the physiological relevance and/or accuracy of this has been strongly challenged.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rseP

Mnemonic

Regulator of sigma E, protease

Synonyms

yaeL, ECK0175, b0176, JW0171, ecf, ecfE[1][2][3], ecfE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.24 minutes 

MG1655: 196546..197898
<gbrowseImage> name=NC_000913:196546..197898 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 199388..200740
<gbrowseImage> name=NC_012967:199388..200740 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 196545..197897
<gbrowseImage> name=NC_012759:196545..197897 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 196546..197898
<gbrowseImage> name=NC_007779:196546..197898 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 170650..172002
<gbrowseImage> name=NC_010473:170650..172002 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rsePH22A,F

H22A,F

Loss of activity

seeded from UniProt:P0AEH1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

12D5

Kohara Phage

Genobase

PMID:3038334

E2B8

Kohara Phage

Genobase

PMID:3038334

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12436

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12436

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944871

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002290

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2331

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000603

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RseP

Synonyms

zinc metallopeptidase[1], B0176[3][1], EcfE[3][1], YaeL[3][1], RseP[3][1] , ecfE, ECK0175, JW0171, yaeL, b0176

Product description

zinc metallopeptidase[3]; RseP zinc protease[2]

Inner membrane zinc RIP metalloprotease; activates RpoE by degrading RseA; multicopy rpoE suppresses rseP mutation[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0004222

metalloendopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004387

F

Seeded from EcoCyc (v14.0)

complete

GO:0004222

metalloendopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008915

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001478

F

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004387

P

Seeded from EcoCyc (v14.0)

complete

GO:0006508

proteolysis

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008915

P

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0645

F

Seeded from EcoCyc (v14.0)

complete

GO:0008237

metallopeptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0482

F

Seeded from EcoCyc (v14.0)

complete

GO:0005887

integral to plasma membrane

PMID:11867724

IDA: Inferred from Direct Assay

C

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MLSFLWDLAS FIVALGVLIT VHEFGHFWVA RRCGVRVERF SIGFGKALWR RTDKLGTEYV
IALIPLGGYV KMLDERAEPV VPELRHHAFN NKSVGQRAAI IAAGPVANFI FAIFAYWLVF
IIGVPGVRPV VGEIAANSIA AEAQIAPGTE LKAVDGIETP DWDAVRLQLV DKIGDESTTI
TVAPFGSDQR RDVKLDLRHW AFEPDKEDPV SSLGIRPRGP QIEPVLENVQ PNSAASKAGL
QAGDRIVKVD GQPLTQWVTF VMLVRDNPGK SLALEIERQG SPLSLTLIPE SKPGNGKAIG
FVGIEPKVIP LPDEYKVVRQ YGPFNAIVEA TDKTWQLMKL TVSMLGKLIT GDVKLNNLSG
PISIAKGAGM TAELGVVYYL PFLALISVNL GIINLFPLPV LDGGHLLFLA IEKIKGGPVS
ERVQDFCYRI GSILLVLLMG LALFNDFSRL
Length

450

Mol. Wt

49.072 kDa

pI

7.2 (calculated)

Extinction coefficient

55,920 - 56,170 (calc based on 8 Y, 8 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

10..446

PF02163 Peptidase family M50

PMID:19920124

Domain

203..277

PF00595 PDZ domain (Also known as DHR or GLGF)

PMID:19920124

<motif_map/>

tmhmm.php?gene=rseP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rseP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128169

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944871

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000603

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AEH1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12436

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12436

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944871

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002290

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2331

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

392

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

135

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

233

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yaeL

File:.jpg

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:196526..196566 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0176 (EcoliWiki Page)

NCBI GEO profiles for rseP

microarray

GenExpDB:b0176 (EcoliWiki Page)

Summary of data for rseP from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (196435..196577) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ11; Well:F1[5]

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Notes

Accessions Related to rseP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12436

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2331

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0176

EcoGene

EcoGene:EG12436

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002290

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000603

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000029412 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

YAEL

From SHIGELLACYC

E. coli O157

YAEL

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF02163 Peptidase family M50

Superfamily (EcoliWiki Page)

SUPERFAMILY:50156

Superfamily (EcoliWiki Page)

SUPERFAMILY:50156

Panther (EcoliWiki Page)

PTHR22939:SF68

Pfam (EcoliWiki Page)

PF00595 PDZ domain (Also known as DHR or GLGF)

EcoCyc

EcoCyc:EG12436

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12436

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002290

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2331

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000603

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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