rpsR:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpsR

Mnemonic

Ribosomal protein, small

Synonyms

ECK4198, b4202, JW4160[1], JW4160

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

95.35 minutes 

MG1655: 4423862..4424089
<gbrowseImage> name=NC_000913:4423862..4424089 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4403994..4404221
<gbrowseImage> name=NC_012967:4403994..4404221 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4362597..4362824
<gbrowseImage> name=NC_012759:4362597..4362824 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4430519..4430746
<gbrowseImage> name=NC_007779:4430519..4430746 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4524224..4524451
<gbrowseImage> name=NC_010473:4524224..4524451 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4423865

Edman degradation

PMID:776663[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Ksg r rpsR MV9

Resistant to

Resistant to Kasugamycin

PMID:363701[3]

The resistance was dependent on the temperature it was grown at; 42C. See Figure 2

Ksgd rpsR MV9

Antibiotic Depedence

Kasugamycin Dependence

PMID:363701[3]

The antibiotic dependence was dependent on the temperature it was grown at; 30 C. See Figure 2b for gel electropherogram. MV 2, 29, 34 and 35 had were also Ksgd, see table 1.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4160

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCACGTTATTTCCGTCGTCG

Primer 2:CCtTGATGGCGATCAGTGTACGG

1G10

Kohara Phage

Genobase

PMID:3038334[5]

7E9

Kohara Phage

Genobase

PMID:3038334[5]

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[6]

est. P1 cotransduction: 2% [7]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[6]

est. P1 cotransduction: 92% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10917

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10917

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000908

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948721

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0910

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013744

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Yaguchi, M (1975) Primary structure of protein S18 from the small Escherichia coli ribosomal subunit. FEBS Lett. 59 217-20 PubMed
  3. 3.0 3.1 Dabbs, ER (1978) Kasugamycin-dependent mutants of Escherichia coli. J. Bacteriol. 136 994-1001 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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