rpmI:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rpmI

Mnemonic

Ribosomal protein, large

Synonyms

ECK1715, b1717, JW1707[1], JW1707

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.75 minutes 

MG1655: 1798023..1797826
<gbrowseImage> name=NC_000913:1797826..1798023 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1777266..1777069
<gbrowseImage> name=NC_012967:1777069..1777266 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1689885..1690082
<gbrowseImage> name=NC_012759:1689885..1690082 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1801713..1801516
<gbrowseImage> name=NC_007779:1801516..1801713 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1888594..1888397
<gbrowseImage> name=NC_010473:1888397..1888594 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1797829

Edman degradation

PMID:3542048[2]
PMID:10739205[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrpmI (Keio:JW1707)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC9443[5]

ΔrpmI788::kan

PMID:16738554[4]

CGSC:102786


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1707

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCCCAAAAATTAAGACCGTACG

Primer 2:CCTGCGTACGGCAGGCACGCGAT

20F5

Kohara Phage

Genobase

PMID:3038334[7]

20H8

Kohara Phage

Genobase

PMID:3038334[7]

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[5]

est. P1 cotransduction: 47% [8]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[5]

est. P1 cotransduction: 24% [8]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11231

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11231

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001209

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946349

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1213

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005732

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kashiwagi, K & Igarashi, K (1987) Nonspecific inhibition of Escherichia coli ornithine decarboxylase by various ribosomal proteins: detection of a new ribosomal protein possessing strong antizyme activity. Biochim. Biophys. Acta 911 180-90 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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