rplT:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplT

Mnemonic

Ribosomal protein, large

Synonyms

ECK1714, b1716, JW1706, pdzA[1], pdzA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

38.74 minutes 

MG1655: 1797773..1797417
<gbrowseImage> name=NC_000913:1797417..1797773 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1777016..1776660
<gbrowseImage> name=NC_012967:1776660..1777016 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1689476..1689832
<gbrowseImage> name=NC_012759:1689476..1689832 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1801463..1801107
<gbrowseImage> name=NC_007779:1801107..1801463 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1888344..1887988
<gbrowseImage> name=NC_010473:1887988..1888344 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1797420

Edman degradation

PMID:381019[2]
PMID:10739205[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1706

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCTCGCGTAAAACGTGGTGT

Primer 2:CCTGCCAGAGCTGCTTTCGCTTT

20F5

Kohara Phage

Genobase

PMID:3038334[5]

20H8

Kohara Phage

Genobase

PMID:3038334[5]

zdi-925::Tn10

Linked marker

CAG12151 = CGSC7385[6]

est. P1 cotransduction: 47% [7]
Synonyms:zdh-925::Tn10

zdj-276::Tn10

Linked marker

CAG18464 = CGSC7387[6]

est. P1 cotransduction: 24% [7]
Synonyms:zdi-276::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10881

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10881

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000872

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945152

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0874

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005729

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Wittmann-Liebold, B & Seib, C (1979) The primary structure of protein L20 from the large subunit of the Escherichia coli ribosome. FEBS Lett. 103 61-5 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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