rplS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplS

Mnemonic

Ribosomal protein, large

Synonyms

ECK2603, b2606, JW2587[1], JW2587

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

59.1 minutes 

MG1655: 2742552..2742205
<gbrowseImage> name=NC_000913:2742205..2742552 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2665937..2665590
<gbrowseImage> name=NC_012967:2665590..2665937 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2628017..2628364
<gbrowseImage> name=NC_012759:2628017..2628364 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2743186..2742839
<gbrowseImage> name=NC_007779:2742839..2743186 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2834317..2833970
<gbrowseImage> name=NC_010473:2833970..2834317 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

2742208

Edman degradation

PMID:339951[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

rplS4

CGSC:73974


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2587

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAGCAACATTATTAAGCAACT

Primer 2:CCGTTAAGACGCTCTTTGATACG

E8F2

Kohara Phage

Genobase

PMID:3038334[4]

22D7

Kohara Phage

Genobase

PMID:3038334[4]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[5]

est. P1 cotransduction: 85% [6]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: % [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10880

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10880

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000871

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947096

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0873

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008574

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Brosius, J & Arfsten, U (1978) Primary structure of protein L19 from the large subunit of Escherichia coli ribosomes. Biochemistry 17 508-16 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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