rplM:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rplM

Mnemonic

Ribosomal protein, large

Synonyms

ECK3220, b3231, JW3200[1], JW3200

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

72.77 minutes 

MG1655: 3376673..3376245
<gbrowseImage> name=NC_000913:3376245..3376673 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3306549..3306121
<gbrowseImage> name=NC_012967:3306121..3306549 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3263393..3263821
<gbrowseImage> name=NC_012759:3263393..3263821 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3378506..3378078
<gbrowseImage> name=NC_007779:3378078..3378506 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3474418..3473990
<gbrowseImage> name=NC_010473:3473990..3474418 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3376245

Edman degradation

PMID:365580[2]
PMID:10739205[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3200

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAAAACTTTTACAGCTAAACC

Primer 2:CCGATGTCAAGAACTTGCGGTTG

7C8

Kohara Phage

Genobase

PMID:3038334[5]

1E3

Kohara Phage

Genobase

PMID:3038334[5]

zhc-6::Tn10

Linked marker

CAG12153 = CGSC7442[6]

est. P1 cotransduction: 65% [7]
Synonyms:zha-6::Tn10

acrF3083::Tn10

Linked marker

CAG12075 = CGSC7447[6]

est. P1 cotransduction: 20% [7]
Synonyms:zhd-3083::Tn10, zhe-3083::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10874

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10874

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000865

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947828

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0867

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010599

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Mende, L (1978) The primary structure of protein L13 from the large subunit of Escherichia coli ribosomes. FEBS Lett. 96 313-6 PubMed
  3. Hoving, S et al. (2000) A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal. Chem. 72 1006-14 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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