priA:Gene Product(s)
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Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
Standard name |
PriA |
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Synonyms |
Primosome factor n' (replication factor Y)[1], B3935[2][1], SrgA[2][1], replication factor Y[2][1], PriA[2][1] , ECK3927, JW3906, srgA, b3935 |
Product description |
Primosome factor Y, also called protein n'; ATP-dependent DNA helicase activity required for recA-dependent stable DNA replication mode; also involved in double-strand break repair[4] |
EC number (for enzymes) |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003677 |
DNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0003677 |
DNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0004386 |
helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0004386 |
helicase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0005658 |
alpha DNA polymerase:primase complex |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006260 |
DNA replication |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006260 |
DNA replication |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006269 |
DNA replication, synthesis of RNA primer |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0008026 |
ATP-dependent helicase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0008270 |
zinc ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006261 |
DNA-dependent DNA replication |
IDA: Inferred from Direct Assay |
P |
required for D-loop dependent DNA replication |
complete | |||
GO:0016787 |
hydrolase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0046872 |
metal ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
GO:0006310 |
DNA recombination |
IMP: Inferred from Mutant Phenotype |
P |
defective in transductional and conjugative recombination |
complete | |||
GO:0010332 |
response to gamma radiation |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
GO:0006302 |
double-strand break repair |
IMP: Inferred from Mutant Phenotype |
P |
complete | ||||
GO:0046677 |
response to antibiotic |
IMP: Inferred from Mutant Phenotype |
P |
hypersensitive to mitomycin C |
complete | |||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
Partner Type | Partner | Notes | References | Evidence |
---|---|---|---|---|
Protein |
Subunits of primosome |
could be indirect |
||
Protein |
rplB |
Experiment(s):EBI-1147108 | ||
Protein |
rpsC |
Experiment(s):EBI-1147108 | ||
Protein |
galF |
Experiment(s):EBI-1147108 | ||
Protein |
rhsA |
Experiment(s):EBI-1147108 | ||
Protein |
rplQ |
Experiment(s):EBI-1147108 | ||
Protein |
tufA |
Experiment(s):EBI-884556 | ||
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
Compartment | Description | Evidence | Reference/Source | Notes |
---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
Name | |
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Sequence |
MPVAHVALPV PLPRTFDYLL PEGMTVKAGC RVRVPFGKQQ ERIGIVVSVS DASELPLNEL KAVVEVLDSE PVFTHSVWRL LLWAADYYHH PIGDVLFHAL PILLRQGRPA ANAPMWYWFA TEQGQAVDLN SLKRSPKQQQ ALAALRQGKI WRDQVATLEF NDAALQALRK KGLCDLASET PEFSDWRTNY AVSGERLRLN TEQATAVGAI HSAADTFSAW LLAGVTGSGK TEVYLSVLEN VLAQGKQALV MVPEIGLTPQ TIARFRERFN APVEVLHSGL NDSERLSAWL KAKNGEAAIV IGTRSALFTP FKNLGVIVID EEHDSSYKQQ EGWRYHARDL AVYRAHSEQI PIILGSATPA LETLCNVQQK KYRLLRLTRR AGNARPAIQH VLDLKGQKVQ AGLAPALITR MRQHLQADNQ VILFLNRRGF APALLCHDCG WIAECPRCDH YYTLHQAQHH LRCHHCDSQR PVPRQCPSCG STHLVPVGLG TEQLEQTLAP LFPGVPISRI DRDTTSRKGA LEQQLAEVHR GGARILIGTQ MLAKGHHFPD VTLVALLDVD GALFSADFRS AERFAQLYTQ VAGRAGRAGK QGEVVLQTHH PEHPLLQTLL YKGYDAFAEQ ALAERRMMQL PPWTSHVIVR AEDHNNQHAP LFLQQLRNLI LSSPLADEKL WVLGPVPALA PKRGGRWRWQ ILLQHPSRVR LQHIINGTLA LINTIPDSRK VKWVLDVDPI EG |
Length |
732 |
Mol. Wt |
81.654 kDa |
pI |
9.1 (calculated) |
Extinction coefficient |
104,850 - 106,225 (calc based on 15 Y, 15 W, and 11 C residues) |
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Domains/Motifs/Modification Sites
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
Resource type | Source | Notes/Reference |
---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
---|---|---|
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ Liu, J et al. (1999) Replication fork assembly at recombination intermediates is required for bacterial growth. Proc. Natl. Acad. Sci. U.S.A. 96 3552-5 PubMed
- ↑ 6.0 6.1 6.2 6.3 Kogoma, T et al. (1996) The DNA replication priming protein, PriA, is required for homologous recombination and double-strand break repair. J. Bacteriol. 178 1258-64 PubMed
- ↑ 7.0 7.1 7.2 7.3 7.4 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 9.0 9.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000166 ! nucleotide binding
- GO:0003676 ! nucleic acid binding
- GO:0003677 ! DNA binding
- GO:0004386 ! helicase activity
- GO:0005524 ! ATP binding
- GO:0005658 ! alpha DNA polymerase:primase complex
- GO:0006260 ! DNA replication
- GO:0006269 ! DNA replication, synthesis of RNA primer
- GO:0008026 ! ATP-dependent helicase activity
- GO:0008270 ! zinc ion binding
- GO:0006261 ! DNA-dependent DNA replication
- GO:0016787 ! hydrolase activity
- GO:0046872 ! metal ion binding
- GO:0006310 ! DNA recombination
- GO:0010332 ! response to gamma radiation
- GO:0006302 ! double-strand break repair
- GO:0046677 ! response to antibiotic
- Proteins