pnp:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
Pnp |
|---|---|
| Synonyms |
polynucleotide phosphorylase/polyadenylase[1], B3164[2][1], Bfl[2][1], Pnp[2][1], polynucleotide phosphorylase[2][1] , bfl, ECK3152, JW5851, b3164 |
| Product description |
Component of polynucleotide phosphorylase[3]; degradosome[3] Polynucleotide phosphorylase; exoribonuclease; PNPase component of RNA degradosome; cold shock protein required for growth at low temperatures[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000175 |
3'-5'-exoribonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000175 |
3'-5'-exoribonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000175 |
3'-5'-exoribonuclease activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0003723 |
RNA binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004654 |
polyribonucleotide nucleotidyltransferase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004654 |
polyribonucleotide nucleotidyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004654 |
polyribonucleotide nucleotidyltransferase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005739 |
mitochondrion |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006396 |
RNA processing |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006396 |
RNA processing |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006396 |
RNA processing |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006402 |
mRNA catabolic process |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006402 |
mRNA catabolic process |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006950 |
response to stress |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016779 |
nucleotidyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of polynucleotide phosphorylase |
could be indirect |
||
|
Protein |
metK |
Experiment(s):EBI-883913 | ||
|
Protein |
recA |
Experiment(s):EBI-883913 | ||
|
Protein |
rplB |
Experiment(s):EBI-883913 | ||
|
Protein |
rplD |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
rplF |
Experiment(s):EBI-883913 | ||
|
Protein |
rplI |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
rplM |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
rpoA |
Experiment(s):EBI-883913 | ||
|
Protein |
rpsC |
Experiment(s):EBI-883913 | ||
|
Protein |
rpsD |
Experiment(s):EBI-883913 | ||
|
Protein |
rpsE |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
rpsG |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
rpsM |
Experiment(s):EBI-883913, EBI-891089 | ||
|
Protein |
srmB |
Experiment(s):EBI-883913 | ||
|
Protein |
rplE |
Experiment(s):EBI-883913 | ||
|
Protein |
pgk |
Experiment(s):EBI-891089, EBI-890008 | ||
|
Protein |
rplA |
Experiment(s):EBI-891089 | ||
|
Protein |
rplO |
Experiment(s):EBI-891089 | ||
|
Protein |
rplQ |
Experiment(s):EBI-891089 | ||
|
Protein |
rplU |
Experiment(s):EBI-891089 | ||
|
Protein |
rplV |
Experiment(s):EBI-891089 | ||
|
Protein |
rplX |
Experiment(s):EBI-891089 | ||
|
Protein |
rpsO |
Experiment(s):EBI-891089 | ||
|
Protein |
rpsR |
Experiment(s):EBI-891089 | ||
|
Protein |
rpsS |
Experiment(s):EBI-891089 | ||
|
Protein |
rpsT |
Experiment(s):EBI-891089 | ||
|
Protein |
hupA |
Experiment(s):EBI-891089 | ||
|
Protein |
rpsS |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplE |
MALDI(Z-score):28.336587 | ||
|
Protein |
rpsR |
LCMS(ID Probability):99.6 | ||
|
Protein |
eno |
LCMS(ID Probability):99.6 MALDI(Z-score):39.324472 | ||
|
Protein |
rplO |
LCMS(ID Probability):99.6 MALDI(Z-score):3.209278 | ||
|
Protein |
rpsO |
LCMS(ID Probability):99.6 | ||
|
Protein |
hupA |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsM |
LCMS(ID Probability):99.6 MALDI(Z-score):20.758343 | ||
|
Protein |
rpsT |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplQ |
LCMS(ID Probability):99.4 | ||
|
Protein |
rplI |
LCMS(ID Probability):99.6 MALDI(Z-score):18.361833 | ||
|
Protein |
rplU |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplX |
LCMS(ID Probability):99.6 | ||
|
Protein |
rpsC |
MALDI(Z-score):35.080787 | ||
|
Protein |
srmB |
MALDI(Z-score):26.241773 | ||
|
Protein |
rplM |
LCMS(ID Probability):99.6 MALDI(Z-score):8.655067 | ||
|
Protein |
rpsD |
LCMS(ID Probability):95.3 MALDI(Z-score):19.991630 | ||
|
Protein |
rplB |
LCMS(ID Probability):94.5 MALDI(Z-score):35.593951 | ||
|
Protein |
rplA |
LCMS(ID Probability):99.6 MALDI(Z-score):16.114612 | ||
|
Protein |
rplD |
LCMS(ID Probability):99.6 MALDI(Z-score):16.007735 | ||
|
Protein |
rhlB |
LCMS(ID Probability):99.6 MALDI(Z-score):37.285496 | ||
|
Protein |
deaD |
MALDI(Z-score):18.761801 | ||
|
Protein |
rluB |
MALDI(Z-score):33.707783 | ||
|
Protein |
Subunits of degradosome |
could be indirect |
| |
| edit table |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MRRRSGINTS AVRYCLRKRK DITLLNPIVR KFQYGQHTVT LETGMMARQA TAAVMVSMDD TAVFVTVVGQ KKAKPGQDFF PLTVNYQERT YAAGRIPGSF FRREGRPSEG ETLIARLIDR PIRPLFPEGF VNEVQVIATV VSVNPQVNPD IVAMIGASAA LSLSGIPFNG PIGAARVGYI NDQYVLNPTQ DELKESKLDL VVAGTEAAVL MVESEAQLLS EDQMLGAVVF GHEQQQVVIQ NINELVKEAG KPRWDWQPEP VNEALNARVA ALAEARLSDA YRITDKQERY AQVDVIKSET IATLLAEDET LDENELGEIL HAIEKNVVRS RVLAGEPRID GREKDMIRGL DVRTGVLPRT HGSALFTRGE TQALVTATLG TARDAQVLDE LMGERTDTFL FHYNFPPYSV GETGMVGSPK RREIGHGRLA KRGVLAVMPD MDKFPYTVRV VSEITESNGS SSMASVCGAS LALMDAGVPI KAAVAGIAMG LVKEGDNYVV LSDILGDEDH LGDMDFKVAG SRDGISALQM DIKIEGITKE IMQVALNQAK GARLHILGVM EQAINAPRGD ISEFAPRIHT IKINPDKIKD VIGKGGSVIR ALTEETGTTI EIEDDGTVKI AATDGEKAKH AIRRIEEITA EIEVGRVYTG KVTRIVDFGA FVAIGGGKEG LVHISQIADK RVEKVTDYLQ MGQEVPVKVL EVDRQGRIRL SIKEATEQSQ PAAAPEAPAA EQGE |
| Length |
734 |
| Mol. Wt |
79.845 kDa |
| pI |
5.3 (calculated) |
| Extinction coefficient |
31,860 - 32,110 (calc based on 14 Y, 2 W, and 2 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 6.13 6.14 6.15 6.16 6.17 6.18 6.19 6.20 6.21 6.22 6.23 6.24 6.25 6.26 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 7.16 7.17 7.18 7.19 7.20 7.21 7.22 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000175 ! 3'-5'-exoribonuclease activity
- GO:0003723 ! RNA binding
- GO:0004654 ! polyribonucleotide nucleotidyltransferase activity
- GO:0005739 ! mitochondrion
- GO:0005829 ! cytosol
- GO:0006396 ! RNA processing
- GO:0006402 ! mRNA catabolic process
- GO:0006950 ! response to stress
- GO:0016020 ! membrane
- GO:0016740 ! transferase activity
- GO:0016779 ! nucleotidyltransferase activity
- Proteins
