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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

php

Mnemonic

Phosphotriesterase homology protein

Synonyms

ECK3366, b3379, JW3342, yhfV[1], yhfV

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

75.56 minutes 

MG1655: 3506612..3505734
<gbrowseImage> name=NC_000913:3505734..3506612 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3436394..3437272
<gbrowseImage> name=NC_012967:3436394..3437272 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3392891..3393769
<gbrowseImage> name=NC_012759:3392891..3393769 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4131826..4132704
<gbrowseImage> name=NC_007779:4131826..4132704 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3604357..3603479
<gbrowseImage> name=NC_010473:3603479..3604357 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3505734

Edman degradation

PMID:9692977[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δphp (Keio:JW3342)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10495[4]

Δphp-786::kan

PMID:16738554[3]

CGSC:102366


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3342

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAGTTTTGATCCGACGGGTTA

Primer 2:CCTTGGAAAAATTGAGAGGGATT

6F9

Kohara Phage

Genobase

PMID:3038334[6]

15C3

Kohara Phage

Genobase

PMID:3038334[6]

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[4]

est. P1 cotransduction: 91% [7]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[4]

est. P1 cotransduction: 5% [7]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G7731

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12917

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004274

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947891

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2753

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011044

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Buchbinder, JL et al. (1998) Biochemical Characterization and Crystallographic Structure of an Escherichia coli Protein from the Phosphotriesterase Gene Family. Biochemistry 37 10860 PubMed
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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