nfuA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
nfuA |
|---|---|
| Mnemonic |
NifU C-terminal domain-like |
| Synonyms |
ECK3401, b3414, JW3377, yhgI, gntY[1], gntY, yhgI |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
76.38 minutes |
MG1655: 3543646..3544221 |
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NC_012967: 3474289..3474864 |
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W3110 |
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W3110: 4094792..4094217 |
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NC_012759: 3430803..3431378 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔgntY (Keio:JW3377) |
deletion |
deletion |
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|
yhgI::Tn5KAN-I-SceI (FB20203) |
Insertion at nt 171 in Minus orientation |
contains pKD46 | |||||
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yhgI::Tn5KAN-I-SceI (FB20203) |
Insertion at nt 171 in Minus orientation |
does not contain pKD46 | |||||
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nfuAC39S |
C39S |
No effect on activity in vivo. Still able to bind a Fe/S cluster in vitro |
seeded from UniProt:P63020 | ||||
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nfuAC149S |
C149S |
Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro |
seeded from UniProt:P63020 | ||||
|
nfuAC44S |
C44S |
No effect on activity in vivo |
seeded from UniProt:P63020 | ||||
|
nfuAC152S |
C152S |
Loss of activity in vivo. Still able to bind a Fe/S cluster in vitro |
seeded from UniProt:P63020 | ||||
|
ΔgntY748::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3377 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATCCGTATTTCCGATGCTGC Primer 2:CCGTAGTAGGAGTGTTCGCCGCG | |
|
Linked marker |
est. P1 cotransduction: 18% [6] | ||
|
Linked marker |
est. P1 cotransduction: 49% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
