nfsB:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

nfsB

Mnemonic

Nitrofurazone sensitivity

Synonyms

nfnB, ECK0570, b0578, JW0567, nfsI, ntr, dprA, G557[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.02 minutes 

MG1655: 604647..603994
<gbrowseImage> name=NC_000913:603994..604647 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 585661..586314
<gbrowseImage> name=NC_012967:585661..586314 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 506754..507407
<gbrowseImage> name=NC_012759:506754..507407 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 604647..603994
<gbrowseImage> name=NC_007779:603994..604647 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

603994

Edman degradation

PMID:1472094[4]
PMID:3060113[5]
PMID:9298646[6]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

nfnB::Tn5KAN-2 (FB20203)

Insertion at nt 461 in Plus orientation

PMID:15262929[7]

E. coli Genome Project:FB20203

does not contain pKD46

nfnB787(del)::kan

PMID:16738554[8]

CGSC:102050

nfsB-2

CGSC:10681

nfnB in strain SSJ-1

Resistant to

Resistant to Nitrofurantoin

PMID:6387400[9]

Strains: SSJ-1 through SSJ-5


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[10]

Status:

Primer 1:

Primer 2:

21A9S

Kohara Phage

Genobase

PMID:3038334[11]

2D12

Kohara Phage

Genobase

PMID:3038334[11]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG50005

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG20151

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002564

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945778

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4146

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0001981

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. Anlezark, GM et al. (1992) The bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB1954)--I. Purification and properties of a nitroreductase enzyme from Escherichia coli--a potential enzyme for antibody-directed enzyme prodrug therapy (ADEPT). Biochem. Pharmacol. 44 2289-95 PubMed
  5. Vasudevan, SG et al. (1988) Dihydropteridine reductase from Escherichia coli. Biochem. J. 255 581-8 PubMed
  6. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  7. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  8. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  9. Sastry, SS & Jayaraman, R (1984) Nitrofurantoin-resistant mutants of Escherichia coli: isolation and mapping. Mol. Gen. Genet. 196 379-80 PubMed
  10. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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