PMID:11237867
| Citation |
Brown, J, Brown, T and Fox, KR (2001) Affinity of mismatch-binding protein MutS for heteroduplexes containing different mismatches. Biochem. J. 354:627-33 |
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| Abstract |
We have used bandshift analysis to measure the interaction between the Escherichia coli mismatch-binding protein MutS and synthetic DNA fragments containing all possible DNA mismatches as well as an unpaired T (DeltaT). The order of affinity is found to be DeltaT>GT>GG>AA approximately TT approximately TC>CA>GA>CC>GC. We find that the affinity for GT mismatches is affected by the flanking sequence and decreases in the order G(n)C(n)>(GC)(n)>A(n)T(n)>(AT)(n). Studies with base analogues show good binding to phiT (where phi represents 1',2'-dideoxyribose), but much weaker binding to Gphi. |
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| Keywords |
Adenosine Triphosphatases; Bacterial Proteins/metabolism; Base Pair Mismatch; DNA/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli Proteins; Guanine/analogs & derivatives; Guanine/metabolism; MutS DNA Mismatch-Binding Protein; Nucleic Acid Heteroduplexes/metabolism; Oligonucleotides/metabolism; Thymine/analogs & derivatives; Thymine/metabolism |
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