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On One Page

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Gene |

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Expression|

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Standard Name	murQ
Gene Synonym(s)	yfeU, ECK2423, b2428, JW2421^[1]^[2], JW2421
Product Desc.	N-acetylmuramic acid 6-phosphate etherase^[2]^[3] N-acetylmuramic acid 6-phosphate (MurNAc 6-P) etherase; GlcNAc 6-phosphate and D-lactate are primary products^[4]
Product Synonyms(s)	predicted PTS component^[1], B2428^[2]^[1], YfeU^[2]^[1], MurQ^[2]^[1] , ECK2423, JW2421, yfeU, b2428
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yfeU^[2]
Regulation/Activity
Quick Links
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Notes

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A lyase-type cleavage mechanism is proposed for MurQ. Allows growth on MurNAc and anhydro-MurNAc as a carbon source. MurQ has SIS phosphosugar binding domain. First gene in putative murQP-yfeW operon. AFMB Structural Genomics target No. 45 (http://afmb.cnrs-mrs.fr/article171.html).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	murQ
Mnemonic	MurNAc
Synonyms	yfeU, ECK2423, b2428, JW2421^[1]^[2], JW2421
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	54.83 minutes	MG1655: 2543795..2544691 <gbrowseImage> name=NC_000913:2543795..2544691 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2474515..2475411 <gbrowseImage> name=NC_012967:2474515..2475411 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2429600..2430496 <gbrowseImage> name=NC_012759:2429600..2430496 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2551219..2552115 <gbrowseImage> name=NC_007779:2551219..2552115 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Description	Reference	Availability	Comments
murQE83A	E83A	10000-fold reduction in catalytic activity			seeded from UniProt:P76535
murQE83Q	E83Q	Loss of catalytic activity			seeded from UniProt:P76535
murQE114Q	E114Q	2000-fold reduction in catalytic activity and 4-fold increase in substrate affinity			seeded from UniProt:P76535
murQD115N	D115N	7-fold reduction in catalytic activity and 1.5-fold increase in substrate affinity			seeded from UniProt:P76535
murQ Glu83Ala	Glu83Ala	Purified MurQ Glu83Ala is catalytically inactive (Table 1).	PMID:18837509
ΔmurQ757::kan			PMID:16738554	CGSC:106256
murQ Glu114Gln	Glu114Gln	Purified MurQ Glu114Gln is dramaticially reduced for catalytic activity (Table 1).	PMID:18837509
murQ Asp115Asn	Asp115Asn	Purified MurQ Asp115Asn has reduced catalytic activity (Table 1).	PMID:18837509
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
5F7	Kohara Phage	Genobase	PMID:3038334
E8E3	Kohara Phage	Genobase	PMID:3038334
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7263	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14162	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003837	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946893	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3914	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008007	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MurQ
Synonyms	predicted PTS component^[1], B2428^[2]^[1], YfeU^[2]^[1], MurQ^[2]^[1] , ECK2423, JW2421, yfeU, b2428
Product description	N-acetylmuramic acid 6-phosphate etherase^[2]^[3] N-acetylmuramic acid 6-phosphate (MurNAc 6-P) etherase; GlcNAc 6-phosphate and D-lactate are primary products^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005529	sugar binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001347	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016835	carbon-oxygen lyase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00068	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016835	carbon-oxygen lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005488	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016836	hydro-lyase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.2.1	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046348	amino sugar catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005488	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:18837509	IPI: Inferred from Physical Interaction	EcoliWiki:murQ	F	Homodimeric.	complete
	GO:0016803	ether hydrolase activity	PMID:18837509	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	mdoD	PMID:16606699	Experiment(s):EBI-1142794
Protein	tdh	PMID:16606699	Experiment(s):EBI-1142794
Protein	skp	PMID:16606699	Experiment(s):EBI-1142794
Protein	rlpB	PMID:16606699	Experiment(s):EBI-1142794
Protein	rpsE	PMID:16606699	Experiment(s):EBI-1142794
Protein	slyD	PMID:16606699	Experiment(s):EBI-1142794
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQFEKMITEG SNTASAEIDR VSTLEMCRII NDEDKTVPLA VERVLPDIAA AIDVIHAQVS GGGRLIYLGA GTSGRLGILD ASECPPTYGV KPGLVVGLIA GGEYAIQHAV EGAEDSREGG VNDLKNINLT AQDVVVGIAA SGRTPYVIAG LEYARQLGCR TVGISCNPGS AVSTTAEFAI TPIVGAEVVT GSSRMKAGTA QKLVLNMLST GLMIKSGKVF GNLMVDVVAT NEKLHVRQVN IVKNATGCSA EQAEAALIAC ERNCKTAIVM VLKNLDAAEA KKRLDQHGGF IRQVLDKE
Length	298
Mol. Wt	31.219 kDa
pI	5.8 (calculated)
Extinction coefficient	7,450 - 8,325 (calc based on 5 Y, 0 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	114..211	PF01380 SIS domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=murQ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130353	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946893	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008007	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76535	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7263	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14162	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946893	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003837	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3914	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	Ecoli K-12	4.785+/-0.1	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.004464286	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	286	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	76	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	222	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yfeU
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2543775..2543815 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2428 (EcoliWiki Page)	NCBI GEO profiles for murQ
microarray	GenExpDB:b2428 (EcoliWiki Page)	Summary of data for murQ from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to murQ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7263	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3914	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2428
EcoGene	EcoGene:EG14162	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003837	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008007	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Canis familiaris	ENSCAFP00000007696 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0190037 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000264717 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000006765 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000019482 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1096345 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000156375 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00026674001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YFEU	From SHIGELLACYC
E. coli O157	YFEU	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01380 SIS domain
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

murQ:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to murQ Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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