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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	mtfA
Gene Synonym(s)	yeeI, ECK1972, b1976, JW1958^[1]^[2], JW1958
Product Desc.	Mlc titration factor^[2]^[3] DgsA(Mlc) anti-repressor; binds DgsA(Mlc) and blocks its repression of ptsG; cytoplasmic^[4]
Product Synonyms(s)	conserved protein^[1], B1976^[2]^[1], YeeI^[2]^[1], MtfA^[2]^[1] , ECK1972, JW1958, yeeI, b1976
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yeeI^[2]
Regulation/Activity
Quick Links
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Notes

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An mtfA mutation has elevated ptsG activity; overproduction increases pstG and dgsA(mlc) expression. The alternate start codon used here is supported in that the 265 aa protein is sufficient for activity (Becker, 2006) and is likely to be used as the in vivo start codon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mtfA
Mnemonic	Mlc titration factor
Synonyms	yeeI, ECK1972, b1976, JW1958^[1]^[2], JW1958
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	44 minutes	MG1655: 2041675..2042472 <gbrowseImage> name=NC_000913:2041675..2042472 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1981650..1982447 <gbrowseImage> name=NC_012967:1981650..1982447 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1934158..1934955 <gbrowseImage> name=NC_012759:1934158..1934955 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2045788..2046585 <gbrowseImage> name=NC_007779:2045788..2046585 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔmtfA751::kan					PMID:16738554	CGSC:106252
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource

Resource Type

Source

Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

9G6

Kohara Phage

Genobase

PMID:3038334

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7063	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003649	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946489	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3159	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006558	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YeeI
Synonyms	conserved protein^[1], B1976^[2]^[1], YeeI^[2]^[1], MtfA^[2]^[1] , ECK1972, JW1958, yeeI, b1976
Product description	Mlc titration factor^[2]^[3] DgsA(Mlc) anti-repressor; binds DgsA(Mlc) and blocks its repression of ptsG; cytoplasmic^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01593	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0963	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0086	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	hemG	PMID:16606699	Experiment(s):EBI-1141533
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1141533
Protein	yqjI	PMID:16606699	Experiment(s):EBI-1141533
Protein	rplP	PMID:16606699	Experiment(s):EBI-1141533
Protein	nadE	PMID:16606699	Experiment(s):EBI-1141533
Protein	rpmA	PMID:16606699	Experiment(s):EBI-1141533
Protein	matC	PMID:16606699	Experiment(s):EBI-1141533
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1141533
Protein	rpsM	PMID:16606699	Experiment(s):EBI-1141533
Protein	rpsC	PMID:16606699	Experiment(s):EBI-1141533
Protein	ahpC	PMID:16606699	Experiment(s):EBI-1141533
Protein	yfeD	PMID:16606699	Experiment(s):EBI-1141533
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1141533
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MQFCSSDEFA SKTMIKWPWK VQESAHQTAL PWQEALSIPL LTCLTEQEQS KLVTLAERFL QQKRLVPLQG FELDSLRSCR IALLFCLPVL ELGLEWLDGF HEVLIYPAPF VVDDEWEDDI GLVHNQRIVQ SGQSWQQGPI VLNWLDIQDS FDASGFNLII HEVAHKLDTR NGDRASGVPF IPLREVAGWE HDLHAAMNNI QEEIELVGEN AASIDAYAAS DPAECFAVLS EYFFSAPELF APRFPSLWQR FCQFYQQDPL QRLHHANDTD SFSATNVH
Length	278
Mol. Wt	31.741 kDa
pI	4.4 (calculated)
Extinction coefficient	55,460 - 56,210 (calc based on 4 Y, 9 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..243	PF06167 Phosphoenolpyruvate:glucose-phosphotransferase regulator		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mtfA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111364	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946489	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006558	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P76346	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7063	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13377	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946489	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003649	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3159	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	151	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	230	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	41^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yeeI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2041655..2041695 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1976 (EcoliWiki Page)	NCBI GEO profiles for mtfA
microarray	GenExpDB:b1976 (EcoliWiki Page)	Summary of data for mtfA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2041486..2041711) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ08; Well:A9^[5]
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Notes

Accessions Related to mtfA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7063	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3159	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1976
EcoGene	EcoGene:EG13377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003649	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006558	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000018363 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S2148	From SHIGELLACYC
E. coli O157	Z3132	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:55486
Pfam (EcoliWiki Page)	PF06167 Phosphoenolpyruvate:glucose-phosphotransferase regulator
PFAM (EcoliWiki Page)	PFAM:PF06167
EcoCyc	EcoCyc:G7063	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13377	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003649	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3159	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006558	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

mtfA:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mtfA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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