mrcA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MrcA

Synonyms

fused penicillin-binding protein 1a: murein transglycosylase[1], murein transpeptidase[1], B3396[2][1], PonA[2][1], MrcA[2][1], PBP1A[2][1] , ECK3383, JW3359, ponA, b3396

Product description

peptidoglycan synthetase; penicillin-binding protein 1A[2][3]

Murein polymerase, PBP1A; bifunctional murein transglycosylase and transpeptidase; penicillin-binding protein 1A; dimeric[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005887

integral to plasma membrane

PMID:319999[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

complete

GO:0008955

peptidoglycan glycosyltransferase activity

PMID:7006606[6]

IDA: Inferred from Direct Assay

F

complete

GO:0008658

penicillin binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001460

F

Seeded from EcoCyc (v14.0)

complete

GO:0009002

serine-type D-Ala-D-Ala carboxypeptidase activity

PMID:7006606[6]

IDA: Inferred from Direct Assay

F

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001264

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011816

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0573

P

Seeded from EcoCyc (v14.0)

complete

GO:0009274

peptidoglycan-based cell wall

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001264

C

Seeded from EcoCyc (v14.0)

complete

GO:0016763

transferase activity, transferring pentosyl groups

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011816

F

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011816

P

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

hflB

PMID:16606699[7]

Experiment(s):EBI-1145791

Protein

alaS

PMID:16606699[7]

Experiment(s):EBI-1145791

Protein

rpsP

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsP

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplP

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplK

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplS

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsM

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsS

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsD

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplO

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

groS

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplR

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsK

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

eno

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):14.416243

Protein

rplI

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):5.817502

Protein

rplB

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpmC

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsN

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

ugpB

PMID:19402753[8]

LCMS(ID Probability):99.3 MALDI(Z-score):8.104456

Protein

ahpC

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

tufB

PMID:19402753[8]

MALDI(Z-score):20.804233

Protein

gadB

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):11.890041

Protein

rplJ

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rpsJ

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

gapA

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):26.091416

Protein

rplE

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):2.650387

Protein

fusA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

hns

PMID:19402753[8]

LCMS(ID Probability):99.5

Protein

yfiD

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

dps

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

tig

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

pflB

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):23.505319

Protein

hdeB

PMID:19402753[8]

LCMS(ID Probability):99.4

Protein

gnd

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

glmS

PMID:19402753[8]

LCMS(ID Probability):98.9

Protein

fur

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

yccJ

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

rplY

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

adk

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

pstS

PMID:19402753[8]

MALDI(Z-score):19.273541

Protein

elaB

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

katG

PMID:19402753[8]

LCMS(ID Probability):99.2

Protein

ribE

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

glyA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

clpB

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

pgm

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

dhaM

PMID:19402753[8]

LCMS(ID Probability):99.2

Protein

talB

PMID:19402753[8]

LCMS(ID Probability):99.5

Protein

aspS

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

ihfA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

luxS

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

fkpA

PMID:19402753[8]

LCMS(ID Probability):98.6

Protein

manA

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

cspC

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

mdh

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

uspD

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

pgk

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):2.192678

Protein

tsf

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

wrbA

PMID:19402753[8]

MALDI(Z-score):21.134951

Protein

yqjD

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

ybaB

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

efp

PMID:19402753[8]

LCMS(ID Probability):99.5

Protein

greA

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

fabG

PMID:19402753[8]

LCMS(ID Probability):99.5

Protein

yfcZ

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

lysU

PMID:19402753[8]

LCMS(ID Probability):98.7 MALDI(Z-score):2.580915

Protein

ansB

PMID:19402753[8]

LCMS(ID Probability):99.5

Protein

rfbB

PMID:19402753[8]

LCMS(ID Probability):99.6

Protein

deoB

PMID:19402753[8]

LCMS(ID Probability):99.0

Protein

osmC

PMID:19402753[8]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Inner Membrane

PMID:3882429[9], PMID:3510188[10]

EchoLocation:mrcA

plasma membrane


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKLNGKFPVK FVKYFLILAV CCILLGAGSI YGLYRYIEPQ LPDVATLKDV RLQIPMQIYS
ADGELIAQYG EKRRIPVTLD QIPPEMVKAF IATEDSRFYE HHGVDPVGIF RAASVALFSG
HASQGASTIT QQLARNFFLS PERTLMRKIK EVFLAIRIEQ LLTKDEILEL YLNKIYLGYR
AYGVGAAAQV YFGKTVDQLT LNEMAVIAGL PKAPSTFNPL YSMDRAVARR NVVLSRMLDE
GYITQQQFDQ TRTEAINANY HAPEIAFSAP YLSEMVRQEM YNRYGESAYE DGYRIYTTIT
RKVQQAAQQA VRNNVLDYDM RHGYRGPANV LWKVGESAWD NNKITDTLKA LPTYGPLLPA
AVTSANPQQA TAMLADGSTV ALSMEGVRWA RPYRSDTQQG PTPRKVTDVL QTGQQIWVRQ
VGDAWWLAQV PEVNSALVSI NPQNGAVMAL VGGFDFNQSK FNRATQALRQ VGSNIKPFLY
TAAMDKGLTL ASMLNDVPIS RWDASAGSDW QPKNSPPQYA GPIRLRQGLG QSKNVVMVRA
MRAMGVDYAA EYLQRFGFPA QNIVHTESLA LGSASFTPMQ VARGYAVMAN GGFLVDPWFI
SKIENDQGGV IFEAKPKVAC PECDIPVIYG DTQKSNVLEN NDVEDVAISR EQQNVSVPMP
QLEQANQALV AKTGAQEYAP HVINTPLAFL IKSALNTNIF GEPGWQGTGW RAGRDLQRRD
IGGKTGTTNS SKDAWFSGYG PGVVTSVWIG FDDHRRNLGH TTASGAIKDQ ISGYEGGAKS
AQPAWDAYMK AVLEGVPEQP LTPPPGIVTV NIDRSTGQLA NGGNSREEYF IEGTQPTQQA
VHEVGTTIID NGEAQELF
Length

858

Mol. Wt

94.52 kDa

pI

6.9 (calculated)

Extinction coefficient

130,640 - 131,140 (calc based on 36 Y, 14 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

427..673

PF00905 Penicillin binding protein transpeptidase domain

PMID:19920124[11]

Domain

54..230

PF00912 Transglycosylase

PMID:19920124[11]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mrcA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:162135911

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947907

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011082

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P02918

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10748

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10748

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947907

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000739

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0741

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Spratt, BG (1977) Properties of the penicillin-binding proteins of Escherichia coli K12,. Eur. J. Biochem. 72 341-52 PubMed
  6. ↑ 6.0 6.1 Ishino, F et al. (1980) Dual enzyme activities of cell wall peptidoglycan synthesis, peptidoglycan transglycosylase and penicillin-sensitive transpeptidase, in purified preparations of Escherichia coli penicillin-binding protein 1A. Biochem. Biophys. Res. Commun. 97 287-93 PubMed
  7. ↑ 7.0 7.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 8.12 8.13 8.14 8.15 8.16 8.17 8.18 8.19 8.20 8.21 8.22 8.23 8.24 8.25 8.26 8.27 8.28 8.29 8.30 8.31 8.32 8.33 8.34 8.35 8.36 8.37 8.38 8.39 8.40 8.41 8.42 8.43 8.44 8.45 8.46 8.47 8.48 8.49 8.50 8.51 8.52 8.53 8.54 8.55 8.56 8.57 8.58 8.59 8.60 8.61 8.62 8.63 8.64 8.65 8.66 8.67 8.68 8.69 8.70 8.71 8.72 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Broome-Smith, JK et al. (1985) The nucleotide sequences of the ponA and ponB genes encoding penicillin-binding protein 1A and 1B of Escherichia coli K12. Eur. J. Biochem. 147 437-46 PubMed
  10. ↑ Barbas, JA et al. (1986) Specific location of penicillin-binding proteins within the cell envelope of Escherichia coli. J. Bacteriol. 165 269-75 PubMed
  11. ↑ 11.0 11.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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