mraZ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

mraZ

Mnemonic

Murein region a

Synonyms

ECK0082, b0081, JW0079, yabB, orfC[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

1.93 minutes 

MG1655: 89634..90092
<gbrowseImage> name=NC_000913:89634..90092 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 92438..92896
<gbrowseImage> name=NC_012967:92438..92896 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 89633..90091
<gbrowseImage> name=NC_012759:89633..90091 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 89634..90092
<gbrowseImage> name=NC_007779:89634..90092 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 63738..64196
<gbrowseImage> name=NC_010473:63738..64196 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

Y41H

Nonsynonomous mutation

PMID:18245285[3]

ASAP

This is a SNP in E. coli K-12 Strain DH10B

yabB::Tn5KAN-I-SceI (FB20011)

Insertion at nt 146 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20011

does not contain pKD46

yabB::Tn5KAN-I-SceI (FB20012)

Insertion at nt 146 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB20012

contains pKD46

mraZ(del) (Keio:JW0079)

deletion

deletion

PMID:16738554[5]

Shigen

CGSC8379[6]

mraZ51::FLKP2(lacZ,kan,Plac)

CGSC:83350

mraZ52(del)

PMID:12142427[7]

CGSC:83353

mraZ787(del)::kan

PMID:16738554[5]

CGSC:101944


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0079

Plasmid clone

Shigen

PMID:16769691[8]

Status:Clone OK

Primer 1:GCCTTCCGGGGAGCAACGTTAGT

Primer 2:CCTAGAGACAAGTCCTGCAGTCG

6C1

Kohara Phage

Genobase

PMID:3038334[9]

6F3

Kohara Phage

Genobase

PMID:3038334[9]

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[6]

est. P1 cotransduction: 81% [10]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[6]

est. P1 cotransduction: 5% [10]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11084

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11084

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001072

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944810

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1076

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000302

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. Merlin, C et al. (2002) Tools for characterization of Escherichia coli genes of unknown function. J. Bacteriol. 184 4573-81 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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