metK:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MetK

Synonyms

methionine adenosyltransferase 1[1], B2942[2][1], MetX[2][1], MetK[2][1] , ECK2937, JW2909, metX, b2942

Product description

MetK S-adenosylmethionine synthetase[2][3];

Component of methionine adenosyltransferase I[2][3]

S-adenosylmethionine synthase; methionine adenosyltransferase; ethionine sensitivity; essential gene[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00086

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0004478

methionine adenosyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00086

F

Seeded from EcoCyc (v14.0)

complete

GO:0004478

methionine adenosyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002133

F

Seeded from EcoCyc (v14.0)

complete

GO:0004478

methionine adenosyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.5.1.6

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00086

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002133

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[5]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0030955

potassium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0630

F

Seeded from EcoCyc (v14.0)

complete

GO:0050897

cobalt ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0170

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of methionine adenosyltransferase I

could be indirect

Protein

fdnH

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

prs

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

ybfC

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

groL

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

phnN

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

speB

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

degQ

PMID:16606699[6]

Experiment(s):EBI-1144372

Protein

dnaK

PMID:15690043[7]

Experiment(s):EBI-881611

Protein

glyQ

PMID:15690043[7]

Experiment(s):EBI-881611

Protein

mreB

PMID:15690043[7]

Experiment(s):EBI-881611

Protein

pstB

PMID:15690043[7]

Experiment(s):EBI-881611

Protein

recA

PMID:15690043[7]

Experiment(s):EBI-881611

Protein

secA

PMID:15690043[7]

Experiment(s):EBI-881611, EBI-880477

Protein

tufA

PMID:15690043[7]

Experiment(s):EBI-881611, EBI-889981, EBI-889076

Protein

recA

PMID:19402753[8]

MALDI(Z-score):28.691364

Protein

pstB

PMID:19402753[8]

MALDI(Z-score):31.949994

Protein

dnaJ

PMID:19402753[8]

LCMS(ID Probability):99.6 MALDI(Z-score):37.245489

Protein

mreB

PMID:19402753[8]

MALDI(Z-score):34.359287

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAKHLFTSES VSEGHPDKIA DQISDAVLDA ILEQDPKARV ACETYVKTGM VLVGGEITTS
AWVDIEEITR NTVREIGYVH SDMGFDANSC AVLSAIGKQS PDINQGVDRA DPLEQGAGDQ
GLMFGYATNE TDVLMPAPIT YAHRLVQRQA EVRKNGTLPW LRPDAKSQVT FQYDDGKIVG
IDAVVLSTQH SEEIDQKSLQ EAVMEEIIKP ILPAEWLTSA TKFFINPTGR FVIGGPMGDC
GLTGRKIIVD TYGGMARHGG GAFSGKDPSK VDRSAAYAAR YVAKNIVAAG LADRCEIQVS
YAIGVAEPTS IMVETFGTEK VPSEQLTLLV REFFDLRPYG LIQMLDLLHP IYKETAAYGH
FGREHFPWEK TDKAQLLRDA AGLK
Length

384

Mol. Wt

41.951 kDa

pI

5.0 (calculated)

Extinction coefficient

39,880 - 40,380 (calc based on 12 Y, 4 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A817

Modification Site

198

phosphorylation site at S198

probability greater than 75%

PMID:17938405[9]

Domain

2..101

PF00438 S-adenosylmethionine synthetase, N-terminal domain

PMID:19920124[10]

Domain

113..231

PF02772 S-adenosylmethionine synthetase, central domain

PMID:19920124[10]

Domain

233..370

PF02773 S-adenosylmethionine synthetase, C-terminal domain

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=metK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130843

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945389

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009650

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A817

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10589

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10589

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945389

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000582

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0584

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  8. ↑ 8.0 8.1 8.2 8.3 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
  10. ↑ 10.0 10.1 10.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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