metE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
metE |
|---|---|
| Mnemonic |
Methionine |
| Synonyms |
ECK3823, b3829, JW3805, metB12[1], metB12 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
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MG1655 |
86.45 minutes |
MG1655: 4011076..4013337 |
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NC_012967: 3977480..3979741 |
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NC_012759: 3900745..3903006 |
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W3110 |
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W3110: 3623628..3621367 |
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DH10B: 4109996..4112257 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
4011079 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔmetE (Keio:JW3805) |
deletion |
deletion |
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metEH641N |
H641N |
Impaired activity, lower affinity for zinc binding |
seeded from UniProt:P25665 | ||||
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metEC726S |
C726S |
Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type |
seeded from UniProt:P25665 | ||||
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metEH641Q |
H641Q |
Impaired activity, lower affinity for zinc binding. Binds homocysteine 2-4x more weakly than wild-type |
seeded from UniProt:P25665 | ||||
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metEC643S |
C643S |
Impaired activity, lower affinity for zinc binding. Binds homocysteine 7x tighter than wild-type |
seeded from UniProt:P25665 | ||||
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metE70 |
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metE163::Tn10 |
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metE94 |
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metE47 |
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metE101 |
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metE192 |
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metE46 |
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metE40 |
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metE68 |
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metE90 |
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ΔmetE774::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3805 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACAATATTGAATCACACCCT Primer 2:CCgCCCCGACGCAAGTTCTGCGC | |
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Kohara Phage |
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metEo-3079::Tn10 |
Linked marker |
est. P1 cotransduction: 92% [8] | |
|
Linked marker |
est. P1 cotransduction: 56% [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ González, JC et al. (1992) Comparison of cobalamin-independent and cobalamin-dependent methionine synthases from Escherichia coli: two solutions to the same chemical problem. Biochemistry 31 6045-56 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
