PMID:6330035
| Citation |
Phillips, TA, VanBogelen, RA and Neidhardt, FC (1984) lon gene product of Escherichia coli is a heat-shock protein. J. Bacteriol. 159:283-7 |
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| Abstract |
The product of the pleiotropic gene lon is a protein with protease activity and has been tentatively identified as protein H94.0 on the reference two-dimensional gel of Escherichia coli proteins. Purified Lon protease migrated with the prominent cellular protein H94.0 in E. coli K-12 strains. Peptide map patterns of Lon protease and H94.0 were identical. A mutant form of the protease had altered mobility during gel electrophoresis. An E. coli B/r strain that is known to be defective in Lon function contained no detectable H94.0 protein under normal growth conditions. Upon a shift to 42 degrees C, however, the Lon protease was induced to high levels in K-12 strains and a small amount of protein became detectable at the H94.0 location in strain B/r. Heat induction of Lon protease was dependent on the normal allele of the regulatory gene, htpR, establishing lon as a member of the high-temperature-production regulon of E. coli. |
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| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Genes; Genes, Bacterial; Heat-Shock Proteins/genetics; Heat-Shock Proteins/isolation & purification; Peptide Fragments/analysis; Temperature |
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