lipA:Gene
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Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Standard name |
lipA |
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Mnemonic |
Lipoate |
Synonyms |
ECK0621, b0628, JW0623, lip[1], lip |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
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MG1655 |
14.19 minutes |
MG1655: 659439..658474 |
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NC_012967: 642799..641834 |
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NC_012759: 561234..562199 |
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W3110 |
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W3110: 660638..659673 |
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DH10B: 598771..597806 |
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DH10B: 712031..711066 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
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Coding Start (SO:0000323) |
658477 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
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ΔlipA (Keio:JW0623) |
deletion |
deletion |
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lipACTRRCPFC94ATRRAPFA |
CTRRCPFC94ATRRAPFA |
Loss of 1 4Fe-4S cluster binding. Loss of activity |
seeded from UniProt:P60716 | ||||
lipACEEASCPNLAEC68AEEASAPNLAEA |
CEEASCPNLAEC68AEEASAPNLAEA |
Loss of 1 4Fe-4S cluster binding. Loss of activity |
seeded from UniProt:P60716 | ||||
lipA9 |
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lipA2 |
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ΔlipA767::kan |
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Notes
Genetic Interactions
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Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
Resource | Resource Type | Source | Notes/Reference |
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JW0623 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGTAAACCCATTGTGATGGA Primer 2:CCCTTAACTTCCATCCCTTTCGC | |
Kohara Phage |
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Linked marker |
est. P1 cotransduction: 87% [9] | ||
Linked marker |
est. P1 cotransduction: 16% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Database | Accession | Notes |
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Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
Name | URL | Comments |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Ollagnier-de Choudens, S & Fontecave, M (1999) The lipoate synthase from Escherichia coli is an iron-sulfur protein. FEBS Lett. 453 25-8 PubMed
- ↑ Miller, JR et al. (2000) Escherichia coli LipA is a lipoyl synthase: in vitro biosynthesis of lipoylated pyruvate dehydrogenase complex from octanoyl-acyl carrier protein. Biochemistry 39 15166-78 PubMed
- ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ Hayden, MA et al. (1993) Biosynthesis of lipoic acid: characterization of the lipoic acid auxotrophs Escherichia coli W1485-lip2 and JRG33-lip9. Biochemistry 32 3778-82 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).