lgt:Gene
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
<protect>
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
lgt |
|---|---|
| Mnemonic |
(pro)Lipoprotein Glyceryl Transferase |
| Synonyms |
ECK2824, b2828, JW2796, umpA[1], umpA |
| edit table |
</protect>
Notes
Location(s) and DNA Sequence
<protect>
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
63.87 minutes |
MG1655: 2964059..2963184 |
||
|
NC_012967: 2860657..2859782 |
||||
|
NC_012759: 2850332..2851207 |
||||
|
W3110 |
|
W3110: 2964693..2963818 |
||
|
DH10B: 3057929..3057054 |
||||
| edit table |
</protect>
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
<protect></protect>
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
lgtW25R |
W25R |
(in SK636, temperature-sensitive) |
Strain variation; seeded from UniProt:P60955 | ||||
|
lgt::Tn5KAN-I-SceI (FB20951) |
Insertion at nt 552 in Plus orientation |
does not contain pKD46 | |||||
|
lgt::Tn5KAN-I-SceI (FB20952) |
Insertion at nt 552 in Plus orientation |
contains pKD46 | |||||
|
lgtY26F |
Y26F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtH7Q |
H7Q |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtH24Q |
H24Q |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtG104S |
G104S |
(in SK634, temperature-sensitive) |
Strain variation; seeded from UniProt:P60955 | ||||
|
lgtL139F |
L139F |
(in SK635, temperature-sensitive) |
Strain variation; seeded from UniProt:P60955 | ||||
|
lgtH289Q |
H289Q |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtY235S |
Y235S |
37% decrease in activity |
seeded from UniProt:P60955 | ||||
|
lgtY282F |
Y282F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtY76F |
Y76F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtH103Q,N |
H103Q,N |
Loss of activity |
seeded from UniProt:P60955 | ||||
|
lgtY62F |
Y62F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtY190F |
Y190F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtH196N,L |
H196N,L |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtH196Q,R |
H196Q,R |
50% decrease in activity |
seeded from UniProt:P60955 | ||||
|
lgtY201F |
Y201F |
No effect |
seeded from UniProt:P60955 | ||||
|
lgtY235F,T |
Y235F,T |
Loss of activity |
seeded from UniProt:P60955 | ||||
| edit table |
<protect></protect>
Notes
Genetic Interactions
<protect>
| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
</protect>
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2796 |
Plasmid clone |
Status:Clone OK Primer 1:GCCACCAGTAGCTATCTGCATTT Primer 2:CCGGAAACGTGTTGCTGTGGGCT | |
|
Kohara Phage |
|||
|
Kohara Phage |
|||
|
Linked marker |
est. P1 cotransduction: 65% [6] | ||
|
Linked marker |
est. P1 cotransduction: % [6] | ||
| edit table |
<protect></protect>
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
<protect></protect>
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
