ispU:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ispU |
|---|---|
| Mnemonic |
Isoprenoid |
| Synonyms |
ECK0173, b0174, JW0169, uppS, rth, yaeS[1], yaeS |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
4.2 minutes |
MG1655: 194903..195664 |
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NC_012967: 197745..198506 |
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NC_012759: 194902..195663 |
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W3110 |
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W3110: 194903..195664 |
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DH10B: 169007..169768 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
194903 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
uppSD26A |
D26A |
Great decrease in activity |
seeded from UniProt:P60472 | ||||
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uppSL137A |
L137A |
Formation predominantly of a C(70) polymer rather than the C(55) polymer |
seeded from UniProt:P60472 | ||||
|
uppSD190A |
D190A |
No effect |
seeded from UniProt:P60472 | ||||
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uppSW75A,F |
W75A,F |
Decrease in activity; reduced affinity for decaprenyl diphosphate substrate analog |
seeded from UniProt:P60472 | ||||
|
uppSD223A |
D223A |
No effect |
seeded from UniProt:P60472 | ||||
|
uppSD218A |
D218A |
Slight decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSW221F |
W221F |
Decrease in affinity for IPP |
seeded from UniProt:P60472 | ||||
|
uppSE81A |
E81A |
Slight decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSW31F |
W31F |
Decrease in activity; reduced affinity for decaprenyl diphosphate substrate analog |
seeded from UniProt:P60472 | ||||
|
uppSW91F |
W91F |
Decrease in affinity for IPP |
seeded from UniProt:P60472 | ||||
|
uppSW149F |
W149F |
Decrease in affinity for IPP |
seeded from UniProt:P60472 | ||||
|
uppSA143V |
A143V |
No effect on polymer length |
seeded from UniProt:P60472 | ||||
|
uppSI62A |
I62A |
Formation predominantly of C(60) and C(65) polymers rather than the C(55) polymer |
seeded from UniProt:P60472 | ||||
|
uppSH103A |
H103A |
No effect |
seeded from UniProt:P60472 | ||||
|
uppSA69L |
A69L |
Produces shorter polymers |
seeded from UniProt:P60472 | ||||
|
uppSS71A |
S71A |
Decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSE73A |
E73A |
Slight decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSN74A |
N74A |
Decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSR77A |
R77A |
Decrease in activity |
seeded from UniProt:P60472 | ||||
|
uppSV105A |
V105A |
Formation predominantly of C(60), C(65) and C(70) polymers rather than the C(55) polymer |
seeded from UniProt:P60472 | ||||
|
uppSD150A |
D150A |
Great decrease in affinity for the substrate |
seeded from UniProt:P60472 | ||||
|
uppSE198A |
E198A |
No effect |
seeded from UniProt:P60472 | ||||
|
uppSW207F |
W207F |
Decrease in affinity for both IPP and decaprenyl diphosphate substrate analog |
seeded from UniProt:P60472 | ||||
|
uppSE213A |
E213A |
Great decrease in activity; reduced affinity for IPP |
seeded from UniProt:P60472 | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0169 |
Plasmid clone |
Status:Clone OK Primer 1:GCCATGTTGTCTGCTACTCAACC Primer 2:CCGGCTGTTTCATCACCGGGCTC | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 12% [6] | ||
|
Linked marker |
est. P1 cotransduction: 22% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Apfel, CM et al. (1999) Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase: cloning, expression, and characterization of the essential uppS gene. J. Bacteriol. 181 483-92 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
