insJ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

insJ

Mnemonic

INsertion Sequence

Synonyms

ECK3545, b3557, JW3527, yi5A[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

 

MG1655: 3718703..3719224
<gbrowseImage> name=NC_000913:3718703..3719224 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3893601..3894122
<gbrowseImage> name=NC_012967:3893601..3894122 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3651138..3651659
<gbrowseImage> name=NC_012967:3651138..3651659 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2775309..2775830
<gbrowseImage> name=NC_012967:2775309..2775830 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 664735..665256
<gbrowseImage> name=NC_012967:664735..665256 source=REL606 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 588542..589063
<gbrowseImage> name=NC_012967:588542..589063 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3607029..3607550
<gbrowseImage> name=NC_012759:3607029..3607550 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3919735..3919214
<gbrowseImage> name=NC_007779:3919214..3919735 source=W3110 preset=GeneLocation </gbrowseImage>

MG1655

80.15 minutes, 80.15 minutes 

MG1655: 3718703..3720072
<gbrowseImage> name=NC_000913:3718703..3720072 source=ecoli preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3816280..3816801
<gbrowseImage> name=NC_010473:3816280..3816801 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

yi5A::Tn5KAN-2 (FB21281)

Insertion at nt 316 in Minus orientation

PMID:15262929[2]

E. coli Genome Project:FB21281

contains pKD46

yi5A::Tn5KAN-2 (FB21282)

Insertion at nt 316 in Minus orientation

PMID:15262929[2]

E. coli Genome Project:FB21282

does not contain pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[3]

Status:

Primer 1:

Primer 2:

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[4]

est. P1 cotransduction: % [5]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 27% [5]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:G7776

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG40010

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948082

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  3. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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