idnK:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

idnK

Mnemonic

Idonate

Synonyms

ECK4261, b4268, JW4225, gntV[1], gntV

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

96.83 minutes, 96.83 minutes 

MG1655: 4492646..4493209
<gbrowseImage> name=NC_000913:4492646..4493209 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4477851..4478414
<gbrowseImage> name=NC_012967:4477851..4478414 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4431381..4431944
<gbrowseImage> name=NC_012759:4431381..4431944 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4499303..4499866
<gbrowseImage> name=NC_007779:4499303..4499866 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4594454..4595017
<gbrowseImage> name=NC_010473:4594454..4595017 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

idnK(del) (Keio:JW4225)

deletion

deletion

PMID:16738554[2]

Shigen

gntK(del) idnK(del)

deletion

deletion

Auxotrophies

double mutant lacks gluconate kinases I and II and fails to grow using gluconate as sole carvon and energy source.

PMID:16299298[3]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4225

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCGGGTGAAAGCTTTATTTT

Primer 2:CCTTCGCAGCGCTGATCTGAGGC

5C4

Kohara Phage

Genobase

PMID:3038334[5]

E4D8

Kohara Phage

Genobase

PMID:3038334[5]

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[6]

est. P1 cotransduction: 11% [7]
Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[6]

est. P1 cotransduction: % [7]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12152

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12152

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002051

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946066

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2072

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013980

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. Leatham, MP et al. (2005) Mouse intestine selects nonmotile flhDC mutants of Escherichia coli MG1655 with increased colonizing ability and better utilization of carbon sources. Infect. Immun. 73 8039-49 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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