holA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

holA

Mnemonic

Holoenzyme

Synonyms

ECK0633, b0640, JW0635[1], JW0635

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

14.44 minutes 

MG1655: 670828..669797
<gbrowseImage> name=NC_000913:669797..670828 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 654189..653158
<gbrowseImage> name=NC_012967:653158..654189 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 572557..573588
<gbrowseImage> name=NC_012759:572557..573588 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 672027..670996
<gbrowseImage> name=NC_007779:670996..672027 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 610160..609129
<gbrowseImage> name=NC_010473:609129..610160 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 723420..722389
<gbrowseImage> name=NC_010473:722389..723420 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

669797

Edman degradation

PMID:1400251[2]
PMID:8505303[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0635

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCATTCGGTTGTACCCGGAACA

Primer 2:CCACCGTCGATAAATACGTCCGC

15D7

Kohara Phage

Genobase

PMID:3038334[5]

crcA280::Tn10

Linked marker

CAG12077 = CGSC7347[6]

est. P1 cotransduction: 58% [7]
Synonyms:zbe-280::Tn10

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[6]

est. P1 cotransduction: 30% [7]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11412

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11412

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001378

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947573

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1384

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002192

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Carter, JR et al. (1992) Molecular cloning, sequencing, and overexpression of the structural gene encoding the delta subunit of Escherichia coli DNA polymerase III holoenzyme. J. Bacteriol. 174 7013-25 PubMed
  3. Dong, Z et al. (1993) DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'. J. Biol. Chem. 268 11758-65 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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