hemG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hemG

Mnemonic

Hemin

Synonyms

ECK3842, b3850, JW3827, yihB[1], yihB

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

86.92 minutes 

MG1655: 4032631..4033176
<gbrowseImage> name=NC_000913:4032631..4033176 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4012981..4013526
<gbrowseImage> name=NC_012967:4012981..4013526 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3922300..3922845
<gbrowseImage> name=NC_012759:3922300..3922845 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3602073..3601528
<gbrowseImage> name=NC_007779:3601528..3602073 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4131551..4132096
<gbrowseImage> name=NC_010473:4131551..4132096 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4032631

Edman degradation

PMID:3611052[2]
PMID:7916647[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

hemG231

PMID:8637896[4]

CGSC:83175

hemG38

PMID:8637896[4] PMID:7638191[5]

CGSC:66694

hemG38

Resistant to

resistant to neomycin

PMID:390093[6]

Strain background: Hfr Cavalli

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3827

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAAAACATTAATTCTTTTCTC

Primer 2:CCTTTCAGCGTCGGTTTGTCGGT

10H11

Kohara Phage

Genobase

PMID:3038334[8]

9B11

Kohara Phage

Genobase

PMID:3038334[8]

fad-751::Tn10

Linked marker

CAG18496 = CGSC7465[9]

est. P1 cotransduction: 84% [10]
Synonyms:fadAB101::Tn10

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[9]

est. P1 cotransduction: 29% [10]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11485

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11485

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001441

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948331

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1448

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012577

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Matsudaira, P (1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes. J. Biol. Chem. 262 10035-8 PubMed
  3. Sasarman, A et al. (1993) Nucleotide sequence of the hemG gene involved in the protoporphyrinogen oxidase activity of Escherichia coli K12. Can. J. Microbiol. 39 1155-61 PubMed
  4. 4.0 4.1 Yang, H et al. (1996) Non-iron porphyrins cause tumbling to blue light by an Escherichia coli mutant defective in hemG. Proc. Natl. Acad. Sci. U.S.A. 93 2459-63 PubMed
  5. Yang, H et al. (1995) Phototaxis away from blue light by an Escherichia coli mutant accumulating protoporphyrin IX. Proc. Natl. Acad. Sci. U.S.A. 92 7332-6 PubMed
  6. Săsărman, A et al. (1979) Mapping of a new hem gene in Escherichia coli K12. J. Gen. Microbiol. 113 297-303 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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