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Product(s) |

Expression|

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Standard Name	gss
Gene Synonym(s)	gss, ECK2982, b2988, JW2956^[1]^[2], gsp, JW2956
Product Desc.	Gsp^[2]^[3]; Component of GSP-CPLX^[2]; fused glutathionylspermidine amidase / glutathionylspermidine synthetase^[3] Glutathionylspermidine synthase/amidase; bifunctional protein^[4]
Product Synonyms(s)	fused glutathionylspermidine amidase^[1], glutathionylspermidine synthetase^[1], B2988^[2]^[1] , Gss, ECK2982, gsp, JW2956, b2988
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gss^[2]
Regulation/Activity
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gss
Mnemonic	Glutathionylspermidine synthase
Synonyms	gss, ECK2982, b2988, JW2956^[1]^[2], gsp, JW2956
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	67.56 minutes	MG1655: 3136544..3134685 <gbrowseImage> name=NC_000913:3134685..3136544 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3070750..3072609 <gbrowseImage> name=NC_012967:3070750..3072609 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3021833..3023692 <gbrowseImage> name=NC_012759:3021833..3023692 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3137178..3135319 <gbrowseImage> name=NC_007779:3135319..3137178 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
Δgsp-729::kan					PMID:16738554	CGSC:99697
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
	Plasmid clone	Shigen	PMID:16769691 Status: Primer 1: Primer 2:
2F9	Kohara Phage	Genobase	PMID:3038334
2B7	Kohara Phage	Genobase	PMID:3038334
5C10	Kohara Phage	Genobase	PMID:3038334
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12882	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12882	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002421	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947474	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2720	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Gss
Synonyms	fused glutathionylspermidine amidase^[1], glutathionylspermidine synthetase^[1], B2988^[2]^[1] , Gss, ECK2982, gsp, JW2956, b2988
Product description	Gsp^[2]^[3]; Component of GSP-CPLX^[2]; fused glutathionylspermidine amidase / glutathionylspermidine synthetase^[3] Glutathionylspermidine synthase/amidase; bifunctional protein^[4]
EC number (for enzymes)	3.5.1.78^[1] 6.3.1.8^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0031225	anchored to membrane				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008884	glutathionylspermidine amidase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:3.5.1.78	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008885	glutathionylspermidine synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.3.1.8	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of GSP-CPLX	could be indirect
Protein	yaeT		PMID:16606699	Experiment(s):EBI-1144499
Protein	nadE		PMID:16606699	Experiment(s):EBI-1144499
Protein	tgt		PMID:16606699	Experiment(s):EBI-1144499
Protein	rspA		PMID:16606699	Experiment(s):EBI-1144499
Protein	yedV		PMID:16606699	Experiment(s):EBI-1144499
Protein	chiA		PMID:16606699	Experiment(s):EBI-1144499
Protein	matC		PMID:16606699	Experiment(s):EBI-1144499
Protein	katG		PMID:16606699	Experiment(s):EBI-1144499
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSKGTTSQDA PFGTLLGYAP GGVAIYSSDY SSLDPQEYED DAVFRSYIDD EYMGHKWQCV EFARRFLFLN YGVVFTDVGM AWEIFSLRFL REVVNDNILP LQAFPNGSPR APVAGALLIW DKGGEFKDTG HVAIITQLHG NKVRIAEQNV IHSPLPQGQQ WTRELEMVVE NGCYTLKDTF DDTTILGWMI QTEDTEYSLP QPEIAGELLK ISGARLENKG QFDGKWLDEK DPLQNAYVQA NGQVINQDPY HYYTITESAE QELIKATNEL HLMYLHATDK VLKDDNLLAL FDIPKILWPR LRLSWQRRRH HMITGRMDFC MDERGLKVYE YNADSASCHT EAGLILERWA EQGYKGNGFN PAEGLINELA GAWKHSRARP FVHIMQDKDI EENYHAQFME QALHQAGFET RILRGLDELG WDAAGQLIDG EGRLVNCVWK TWAWETAFDQ IREVSDREFA AVPIRTGHPQ NEVRLIDVLL RPEVLVFEPL WTVIPGNKAI LPILWSLFPH HRYLLDTDFT VNDELVKTGY AVKPIAGRCG SNIDLVSHHE EVLDKTSGKF AEQKNIYQQL WCLPKVDGKY IQVCTFTVGG NYGGTCLRGD ESLVIKKESD IEPLIVVKK
Length	619
Mol. Wt	70.532 kDa
pI	5.1 (calculated)
Extinction coefficient	127,770 - 128,895 (calc based on 23 Y, 17 W, and 9 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	223..617	PF03738 Glutathionylspermidine synthase	PMID:19920124
Domain	39..176	PF05257 CHAP domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gsp taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130888	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947474	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009804	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AES0	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12882	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12882	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947474	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002421	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2720	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.07E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1111	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	558	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	830	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gss
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3136524..3136564 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2988 (EcoliWiki Page)	NCBI GEO profiles for gsp
microarray	GenExpDB:b2988 (EcoliWiki Page)	Summary of data for gsp from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to gsp Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12882	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2720	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2988
EcoGene	EcoGene:EG12882	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002421	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	GSP	From SHIGELLACYC
E. coli O157	GSP	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03738 Glutathionylspermidine synthase
Superfamily (EcoliWiki Page)	SUPERFAMILY:52440
Superfamily (EcoliWiki Page)	SUPERFAMILY:54001
Superfamily (EcoliWiki Page)	SUPERFAMILY:56059
Pfam (EcoliWiki Page)	PF05257 CHAP domain
EcoCyc	EcoCyc:EG12882	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12882	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002421	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2720	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009804	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

gss:On One Page

Quickview

Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gsp Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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