gltS:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

gltS

Mnemonic

Glutamate

Synonyms

ECK3643, b3653, JW3628, gltC[1], gltC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

82.45 minutes, 82.45 minutes 

MG1655: 3826688..3825483
<gbrowseImage> name=NC_000913:3825483..3826688 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3767707..3766502
<gbrowseImage> name=NC_012967:3766502..3767707 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3713816..3715021
<gbrowseImage> name=NC_012759:3713816..3715021 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3811750..3812955
<gbrowseImage> name=NC_007779:3811750..3812955 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3924272..3923067
<gbrowseImage> name=NC_010473:3923067..3924272 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔgltS (Keio:JW3628)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10666[3]

gltS::Tn5KAN-I-SceI (FB21372)

Insertion at nt 568 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21372

contains pKD46

gltS::Tn5KAN-I-SceI (FB21373)

Insertion at nt 568 in Plus orientation

PMID:15262929[4]

E. coli Genome Project:FB21373

does not contain pKD46

gltS10

CGSC:8153

ΔgltS757::kan

PMID:16738554[2]

CGSC:101212


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3628

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTTTCATCTCGATACTTTAGC

Primer 2:CCACCGGCAAAAATCGGCAACAT

7F3

Kohara Phage

Genobase

PMID:3038334[6]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[3]

est. P1 cotransduction: % [7]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[3]

est. P1 cotransduction: 93% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10406

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10406

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000399

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948166

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0401

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011941

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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