glnS:Gene

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glnS

Mnemonic

Glutamine

Synonyms

ECK0668, b0680, JW0666[1], JW0666

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

15.2 minutes 

MG1655: 705316..706980
<gbrowseImage> name=NC_000913:705316..706980 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 688188..689852
<gbrowseImage> name=NC_012967:688188..689852 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 608076..609740
<gbrowseImage> name=NC_012759:608076..609740 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 706515..708179
<gbrowseImage> name=NC_007779:706515..708179 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 757908..759572
<gbrowseImage> name=NC_010473:757908..759572 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

705319

Edman degradation

PMID:6749844[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

glnS1(ts)

temperature sensitive

CGSC:6278


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0666

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGTGAGGCAGAAGCCCGCCC

Primer 2:CCCTCGCCTACTTTCGCCCAGGT

3A6

Kohara Phage

Genobase

PMID:3038334[5]

asnB3057::Tn10

Linked marker

CAG18433 = CGSC7349[6]

est. P1 cotransduction: 85% [7]
Synonyms:zbf-3057::Tn10 nnnThe Tn10 in CAG18433 is inserted in the asnB regulatory region, 4 bp before the start of the asnB ORF.

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[6]

est. P1 cotransduction: 1% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10390

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10390

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000383

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945310

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0385

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002317

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Hoben, P et al. (1982) Escherichia coli glutaminyl-tRNA synthetase. II. Characterization of the glnS gene product. J. Biol. Chem. 257 11644-50 PubMed
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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