ftsH:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
ftsH |
|---|---|
| Mnemonic |
Filamentation, temperature sensitive |
| Synonyms |
ftsH, ECK3167, b3178, JW3145, mrsC, tolZ, std, G528, hflB[1][2][3], hflB |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
71.62 minutes |
MG1655: 3324957..3323023 |
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NC_012967: 3260235..3262169 |
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NC_012759: 3210171..3212105 |
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W3110 |
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W3110: 3326790..3324856 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
hflB::Tn5KAN-I-SceI (FB21462) |
Insertion at nt 1913 in Minus orientation |
does not contain pKD46 | |||||
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hflB::Tn5KAN-I-SceI (FB21462) |
Insertion at nt 1913 in Minus orientation |
contains pKD46 | |||||
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ftsH0 |
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xxx |
xxxx |
xxxxx |
pmid: 2222222 |
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ftsH1 |
Growth Phenotype |
after 2 hours at 42C the mutation shows inability to form colonies and cell elongation and amount of PBP3 is slightly reduced Phenotype is similar to ftsH101 at 37C and higher |
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ftsH41 |
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ftsH101 |
allows significant export of PhoA across membrane |
partially suppressed by overproduction of HtpG also known as std101 | |||||
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tolZ21 in UM21 |
Resistant to |
Increased Resistance toward Colicin E2 |
The tolZ mutation caused increased Resistance toward E2. This was seen in Strains UM31, -34 & -37 with their respective mutations. See table 3. | ||||
|
tolZ31 in UM31 |
Resistant to |
Increased Resistance toward Colicin E3 |
The tolZ mutation caused increased Resistance toward E3. This was seen in Strains UM21, -34 & -37 with their respective mutations. See table 3. | ||||
|
tolZ34 in UM34 |
Resistant to |
Increased Resistance toward Colicin D |
The tolZ mutation caused increased Resistance toward D. This was seen in Strains UM21, -31 & -37 with their respective mutations. See table 3. | ||||
|
tolZ21 in Strain UM21 |
Resistant to |
Resistance to Colicin Ia |
The tolZ mutation conferred resistance toward Ia. This was also seen in UM31, -34 & 37 with their respective tolZ mutations. See table 3. | ||||
|
tolZ37 in Strain UM37 |
Resistant to |
Resistance to Colicin Ib |
The tolZ mutation conferred resistance toward Ib. This was also seen in UM21, -31 & 34 with their respective tolZ mutations. See table 3. | ||||
|
tolZ21 in UM21 |
Resistant to |
Decreased resistance toward Ampicillin. |
The tolZ21 mutation caused decreased resistance to Ampicillin. This was also seen in Strains UM31, -34 & 37. See table 5. | ||||
|
tolZ21 in UM21 |
Resistant to |
Decreased resistance toward Neomycin. |
The tolZ21 mutation caused decreased resistance to Neomycin. This was also seen in Strains UM31, -34 & 37. See table 5. | ||||
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tolZ21 in UM21 |
Resistant to |
Decreased resistance toward EDTA. |
The tolZ21 mutation caused decreased resistance to EDTA. This was also seen in Strains UM31, -34 & 37. See table 5. | ||||
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tolZ21 in Strain UM21 |
Growth Phenotype |
Reduced Aerobic growth |
The tolZ mutation caused decrease growth under aerobic conditions, approximately to 45 percent of the wild type. See figure 1. | ||||
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tolZ21 in Strain UM21 |
Growth Phenotype |
Reduced Anaerobic growth |
The tolZ mutation caused decrease growth under aerobic conditions, approximately to 60 percent of the wild type. See figure 1. | ||||
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tolZ21 in strain UM21 |
Metabolic Uptake |
Decrease Uptake of Various amino acids. |
Decreased uptake of the proline, glutamine and methionine. See figure 2. | ||||
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tolZ21 in UM21 |
Metabolic Uptake |
Reduced uptake of Sugars |
The tolZ mutation caused reduced uptake of Thiomethyl-beta-D-galactoside and alpha-methyl-D-glucoside. See Figure 2. | ||||
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tolZ21 in UM21 |
Metabolic Uptake |
Decreased uptake of triphenylmethylphosphonium |
The tolZ mutation caused reduced uptake of the ion triphenylmethylphosphonium. See figure 2. | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
Plasmid clone |
Status: Primer 1: Primer 2: | ||
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Kohara Phage |
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Kohara Phage |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Begg, KJ et al. (1992) Escherichia coli mutant Y16 is a double mutant carrying thermosensitive ftsH and ftsI mutations. J. Bacteriol. 174 2416-7 PubMed
- ↑ 6.0 6.1 Akiyama, Y et al. (1994) Involvement of FtsH in protein assembly into and through the membrane. I. Mutations that reduce retention efficiency of a cytoplasmic reporter. J. Biol. Chem. 269 5218-24 PubMed
- ↑ 7.0 7.1 Shirai, Y et al. (1996) Suppression of ftsH mutant phenotypes by overproduction of molecular chaperones. J. Bacteriol. 178 1141-5 PubMed
- ↑ 8.0 8.1 8.2 Chatfield, M (1984) Books for health care managers. Health Care Manage Rev 9 77-9 PubMed
- ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 9.7 9.8 9.9 Matsuzawa, H et al. (1984) Escherichia coli K-12 tolZ mutants tolerant to colicins E2, E3, D, Ia, and Ib: defect in generation of the electrochemical proton gradient. J. Bacteriol. 160 733-9 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 11.0 11.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
