dxr:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
dxr |
|---|---|
| Mnemonic |
Deoxy-xyulose-P reductoisomerase |
| Synonyms |
ECK0172, b0173, JW0168, ispC, yaeM[1], yaeM |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
4.17 minutes |
MG1655: 193521..194717 |
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NC_012759: 193520..194716 |
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W3110 |
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W3110: 193521..194717 |
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DH10B: 167625..168821 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
dxr::aphII |
insertion of aphII gene at BalI site in dxr |
Auxotrophies |
2-C-methylerythritol auxotrophy |
Defective in the nonmevalonate pathway for isoprenoid synthesis. aphII was from Tn5 and confers kanamycin resistance | |||
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dxrH153Q |
H153Q |
Increase in KM for substrate. Reduces activity 35-fold |
seeded from UniProt:P45568 | ||||
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dxrH209Q |
H209Q |
Increase in KM for substrate. Reduces activity 5000-fold |
seeded from UniProt:P45568 | ||||
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dxrE231K |
E231K |
No effect on KM for substrate. Reduces activity by over 99.9% |
seeded from UniProt:P45568 | ||||
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dxrH257Q |
H257Q |
Strong increase in KM for substrate. Loss of activity |
seeded from UniProt:P45568 | ||||
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dxrG14D |
G14D |
Loss of solubility and activity |
seeded from UniProt:P45568 | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0168 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAGCAACTCACCATTCTGGG Primer 2:CCGCTTGCGAGACGCATCACCTC | |
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Kohara Phage |
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Kohara Phage |
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Kohara Phage |
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|
Linked marker |
est. P1 cotransduction: 14% [6] | ||
|
Linked marker |
est. P1 cotransduction: 20% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Kuzuyama, T et al. (1999) Construction and characterization of Escherichia coli disruptants defective in the yaeM gene. Biosci. Biotechnol. Biochem. 63 776-8 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 4.0 4.1 4.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
