PMID:10361694
| Citation |
Kuzuyama, T, Takahashi, S and Seto, H (1999) Construction and characterization of Escherichia coli disruptants defective in the yaeM gene. Biosci. Biotechnol. Biochem. 63:776-8 |
|---|---|
| Abstract |
Escherichia coli disruptants defective in the yaeM gene, which is located at 4.2 min on the chromosome map, were constructed and characterized. The disruptants showed auxotrophy for 2-C-methylerythritol, a free alcohol of 2-C-methyl-D-erythritol 4-phosphate that is a biosynthetic precursor in the nonmevalonate pathway. This result clearly shows that the yaeM gene is indeed involved in this pathway in E. coli. |
| Links | |
| Keywords |
Aldose-Ketose Isomerases/biosynthesis; Aldose-Ketose Isomerases/genetics; Blotting, Southern; Catalysis; Cloning, Molecular; DNA, Fungal/biosynthesis; DNA, Fungal/genetics; Erythritol/analogs & derivatives; Erythritol/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial/genetics; Multienzyme Complexes/biosynthesis; Multienzyme Complexes/genetics; Mutation/genetics; Mutation/physiology; Oxidoreductases/biosynthesis; Oxidoreductases/genetics; Pentosephosphates/metabolism; Phenotype; Sugar Phosphates/metabolism |
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