dipZ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbD

Mnemonic

Disulfide bond

Synonyms

dipZ, ECK4130, b4136, JW5734, cutA2, cycZ, htrI, dsbD[1][2], htrI

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94 minutes, 94 minutes 

MG1655: 4363065..4361368
<gbrowseImage> name=NC_000913:4361368..4363065 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4369720..4368023
<gbrowseImage> name=NC_007779:4368023..4369720 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4463427..4461730
<gbrowseImage> name=NC_010473:4461730..4463427 source=DH10B preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4343763..4342066
<gbrowseImage> name=NC_012967:4342066..4343763 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4300103..4301800
<gbrowseImage> name=NC_012759:4300103..4301800 source=BW2952 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdipZ762::kan

PMID:16738554[3]

CGSC:100610

dipZ4::Cm

PMID:8917542[4] PMID:9352906[5]

CGSC:65873

ΔdsbD

PMID:9335267[6], PMID:10545108[7]

This strain contains an internal in-frame deletion of 536 codons of the dsbD gene. We replaced the deleted DNA sequences by an EcoRI site,

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[8]

Status:

Primer 1:

Primer 2:

21H11

Kohara Phage

Genobase

PMID:3038334[9]

5G7

Kohara Phage

Genobase

PMID:3038334[9]

9B1

Kohara Phage

Genobase

PMID:3038334[9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12178

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12178

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002071

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948649

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2095

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013539

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. Rietsch, A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 13048-53 PubMed
  5. Rietsch, A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179 6602-8 PubMed
  6. Link, AJ et al. (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179 6228-37 PubMed
  7. Stewart, EJ et al. (1999) Six conserved cysteines of the membrane protein DsbD are required for the transfer of electrons from the cytoplasm to the periplasm of Escherichia coli. EMBO J. 18 5963-71 PubMed
  8. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. 9.0 9.1 9.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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