dipZ:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dsbD

Mnemonic

Disulfide bond

Synonyms

dipZ, ECK4130, b4136, JW5734, cutA2, cycZ, htrI, dsbD[1][2], htrI

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Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94 minutes, 94 minutes 

MG1655: 4363065..4361368
<gbrowseImage> name=NC_000913:4361368..4363065 source=MG1655 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4369720..4368023
<gbrowseImage> name=NC_007779:4368023..4369720 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4463427..4461730
<gbrowseImage> name=NC_010473:4461730..4463427 source=DH10B preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4343763..4342066
<gbrowseImage> name=NC_012967:4342066..4343763 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4300103..4301800
<gbrowseImage> name=NC_012759:4300103..4301800 source=BW2952 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdipZ762::kan

PMID:16738554[3]

CGSC:100610

dipZ4::Cm

PMID:8917542[4] PMID:9352906[5]

CGSC:65873

ΔdsbD

PMID:9335267[6], PMID:10545108[7]

This strain contains an internal in-frame deletion of 536 codons of the dsbD gene. We replaced the deleted DNA sequences by an EcoRI site,

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[8]

Status:

Primer 1:

Primer 2:

21H11

Kohara Phage

Genobase

PMID:3038334[9]

5G7

Kohara Phage

Genobase

PMID:3038334[9]

9B1

Kohara Phage

Genobase

PMID:3038334[9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12178

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12178

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002071

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948649

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2095

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013539

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Rietsch, A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 13048-53 PubMed
  5. ↑ Rietsch, A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179 6602-8 PubMed
  6. ↑ Link, AJ et al. (1997) Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179 6228-37 PubMed
  7. ↑ Stewart, EJ et al. (1999) Six conserved cysteines of the membrane protein DsbD are required for the transfer of electrons from the cytoplasm to the periplasm of Escherichia coli. EMBO J. 18 5963-71 PubMed
  8. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  9. ↑ 9.0 9.1 9.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed

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